FlyBase Allele Report: Dmel\Dscam1[05518]

General Information

Symbol

Dmel\Dscam1⁰⁵⁵¹⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0008092

Feature type

allele

Associated gene

Dmel\Dscam1

Associated Insertion(s)

P{PZ}Dscam1⁰⁵⁵¹⁸

Carried in Construct

Also Known As

Dscam^P1, dscam1^p, Dscam^p

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{PZ}Dscam1⁰⁵⁵¹⁸

Cytology

Description

The insertion is beyond exon 4 of the Dscam gene.

(Schmucker et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,365,649..7,365,649 [+]

(Alavi et al., 2016, Chen et al., 2006, Zhu et al., 2006, Wang et al., 2002, Schmucker et al., 2000, Spradling et al., 1999, Roote, 1995.9.26, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Down syndrome

CEA with fra⁴

(Hernández et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone

(Okumura et al., 2015)

axon

(Schmucker et al., 2000)

Bolwig nerve

(Andrews et al., 2008)

Bolwig nerve (with Df(2R)cos-2)

(Schmucker et al., 2000)

embryonic/larval frontal nerve | embryonic stage

(Hernández et al., 2023)

embryonic heart cardioblast

(Raza and Jacobs, 2016)

embryonic heart cardioblast (with Dscam1¹)

(Raza and Jacobs, 2016)

filopodium | embryonic stage

(Raza and Jacobs, 2016)

filopodium | embryonic stage (with Dscam1¹)

(Raza and Jacobs, 2016)

frontal ganglion | embryonic stage

(Hernández et al., 2023)

lamellipodium | embryonic stage

(Raza and Jacobs, 2016)

lamellipodium | embryonic stage (with Dscam1¹)

(Raza and Jacobs, 2016)

larval dorsal multidendritic neuron ddaC

(Kim et al., 2013)

larval longitudinal connective

(Schmucker et al., 2000)

larval s-LNv neuron

(Liu et al., 2024)

larval s-LNv neuron (with Dscam1²¹)

(Liu et al., 2024)

neuron projection | larval stage

(Liu et al., 2024)

neuron projection | larval stage (with Dscam1²¹)

(Liu et al., 2024)

symmetrical commissure

(Andrews et al., 2008, Schmucker et al., 2000)

synapse

(Kim et al., 2013)

Detailed Description

Statement

Reference

Dscam1⁰⁵⁵¹⁸ homozygous embryonic heart cardioblasts show significant decreases in filopodial and lamellopodial activities, but not in migration velocity, as compared to controls. Dscam1¹ heterozygous embryonic heart cardioblasts also show a significant decrease in filopodial activity, but not in lamellopodial activity or migration velocity, as compared to controls.

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ mutant DL1 olfactory projection neuron clones exhibit loss of dorsal branches in axons. There is no increase in the number of dendrites spilling-over to the outside of the antennal lobe compared to wild type.

Generation of Dscam1⁰⁵⁵¹⁸ mutant mushroom body clones causes loss of the β lobe.

(Okumura et al., 2015)

Single C4 da neurons homozygous for Dscam1⁰⁵⁵¹⁸, exhibit markedly reduced presynaptic arbor growth.

(Kim et al., 2013)

Levels of apoptosis are not increased in the thoracic and abdominal segments of the Dscam⁰⁵⁵¹⁸ mutant ventral nerve cord.

(Newquist et al., 2013)

27.5% of homozygous Dscam⁰⁵⁵¹⁸ embryos show defects in axon guidance in the Bolwig's nerve.

30% of segments fail to separate the anterior and posterior commissures correctly in Dscam⁰⁵⁵¹⁸ embryos.

(Andrews et al., 2008)

About 5% of heterozygotes exhibit some Bolwig's nerve targeting defects. Dscam⁰⁵⁵¹⁸ hemizygous mutants exhibit mild to severe disorganisation of embryonic axon tracts. Breaks in the connectives, predominantly of the outer two fascicles are observed. In addition axon bundles aberrantly cross the midline. Some 53%-58% of Bolwig's nerve (BN) projections are defective in Dscam¹/Df(2R)cos-2 hemizygotes. In half of the "abnormal projections" the entire nerve mistargets, whereas in the remainder only a subset of axons does. Many otherwise normal projections show abnormal expansion of BN terminus at P2. These phenotypes are 53-58% penetrant.

(Schmucker et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has abnormal cell migration | embryonic stage phenotype, non-enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has abnormal cell migration | embryonic stage phenotype, non-enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ has abnormal neuroanatomy | cell autonomous | somatic clone phenotype, non-enhanceable by TBCD[+]/Tbcd¹

(Okumura et al., 2015)

Dscam1⁰⁵⁵¹⁸, fra⁴ has abnormal neuroanatomy phenotype, non-enhanceable by Dscam3^c02826/Dscam3^c02826

(Andrews et al., 2008)

Suppressed by

Statement

Reference

Dscam1⁰⁵⁵¹⁸, fra⁴ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^scrt-11-6/Dscam1^{7.27.25.1.UAS.cZa}

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, fra⁴ has abnormal neuroanatomy | embryonic stage phenotype, suppressible by Scer\GAL4^scrt-11-6/Dscam1^{UAS.1.30.30.2}

(Hernández et al., 2023)

Enhancer of

Statement

Reference

Dscam1⁰⁵⁵¹⁸ is an enhancer | cell autonomous | somatic clone of abnormal neuroanatomy | cell non-autonomous | dominant phenotype of Tbcd¹

(Okumura et al., 2015)

Dscam1⁰⁵⁵¹⁸/Dscam3^c02826 is an enhancer of abnormal neuroanatomy phenotype of fra^unspecified

(Andrews et al., 2008)

Suppressor of

Statement

Reference

Dscam1⁰⁵⁵¹⁸/Dscam1[+] is a suppressor of abnormal cell migration | dominant | embryonic stage phenotype of robo1¹

(Raza and Jacobs, 2016)

Other

Statement

Reference

Dscam1⁰⁵⁵¹⁸, fra⁴ has abnormal neuroanatomy | embryonic stage phenotype

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, robo1⁴ has abnormal neuroanatomy | embryonic stage phenotype

(Hernández et al., 2023)

Df(1)NP5/+, Dscam1⁰⁵⁵¹⁸ has abnormal neuroanatomy | dominant phenotype

(Andrews et al., 2008)

Df(1)NetAB^Δ/+, Dscam1⁰⁵⁵¹⁸ has abnormal neuroanatomy | dominant phenotype

(Andrews et al., 2008)

Dscam1⁰⁵⁵¹⁸/Dscam[+], fra⁴ has abnormal neuroanatomy | dominant phenotype

(Andrews et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has filopodium | embryonic stage phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has lamellipodium | embryonic stage phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has embryonic heart cardioblast phenotype, enhanceable by fra³/fra[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has filopodium | embryonic stage phenotype, enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has lamellipodium | embryonic stage phenotype, enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1¹ has embryonic heart cardioblast phenotype, enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ has Bolwig nerve phenotype, enhanceable by dock³

(Schmucker et al., 2000)

Dscam1⁰⁵⁵¹⁸ has Bolwig nerve phenotype, enhanceable by Pak⁶

(Schmucker et al., 2000)

NOT Enhanced by

Statement

Reference

Dscam1⁰⁵⁵¹⁸ has filopodium | embryonic stage phenotype, non-enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ has lamellipodium | embryonic stage phenotype, non-enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ has embryonic heart cardioblast phenotype, non-enhanceable by robo1¹/robo1[+]

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ has adult antennal lobe projection neuron DL1 adPN | cell autonomous | somatic clone phenotype, non-enhanceable by TBCD[+]/Tbcd¹

(Okumura et al., 2015)

Dscam1⁰⁵⁵¹⁸, fra⁴ has Bolwig nerve phenotype, non-enhanceable by Dscam3^c02826/Dscam3^c02826

(Andrews et al., 2008)

Suppressed by

Statement

Reference

Dscam1⁰⁵⁵¹⁸, fra⁴ has commissure | embryonic stage phenotype, suppressible by Scer\GAL4^scrt-11-6/Dscam1^{7.27.25.1.UAS.cZa}

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, fra⁴ has commissure | embryonic stage phenotype, suppressible by Scer\GAL4^scrt-11-6/Dscam1^{UAS.1.30.30.2}

(Hernández et al., 2023)

Enhancer of

Statement

Reference

Dscam1⁰⁵⁵¹⁸ is an enhancer | cell autonomous | somatic clone of adult antennal lobe projection neuron DL1 adPN | cell non-autonomous phenotype of Tbcd¹

(Okumura et al., 2015)

Dscam1⁰⁵⁵¹⁸/Dscam3^c02826 is an enhancer of SP1 neuron phenotype of fra^unspecified

(Andrews et al., 2008)

Dscam1⁰⁵⁵¹⁸ is an enhancer of Bolwig nerve phenotype of dock³

(Schmucker et al., 2000)

Dscam1⁰⁵⁵¹⁸ is an enhancer of Bolwig nerve phenotype of Pak⁶

(Schmucker et al., 2000)

NOT Enhancer of

Statement

Reference

Dscam1⁰⁵⁵¹⁸/Dscam1[+] is a non-enhancer of lamellipodium | embryonic stage phenotype of robo1¹

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1[+] is a non-enhancer of embryonic heart cardioblast | embryonic stage phenotype of robo1¹

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸/Dscam1[+] is a non-enhancer of filopodium | embryonic stage phenotype of robo1¹

(Raza and Jacobs, 2016)

Suppressor of

Statement

Reference

Dscam1⁰⁵⁵¹⁸/Dscam1[+] is a suppressor of embryonic heart cardioblast | embryonic stage phenotype of robo1¹

(Raza and Jacobs, 2016)

Other

Statement

Reference

Dscam1⁰⁵⁵¹⁸, fra⁴ has commissure | embryonic stage phenotype

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, fra⁴ has embryonic/larval frontal nerve | embryonic stage | absent phenotype

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, fra⁴ has frontal ganglion | embryonic stage phenotype

(Hernández et al., 2023)

Dscam1⁰⁵⁵¹⁸, robo1⁴ has embryonic/larval frontal nerve | embryonic stage phenotype

(Hernández et al., 2023)

Df(1)NP5/+, Dscam1⁰⁵⁵¹⁸ has Bolwig nerve phenotype

(Andrews et al., 2008)

Df(1)NetAB^Δ/+, Dscam1⁰⁵⁵¹⁸ has Bolwig nerve phenotype

(Andrews et al., 2008)

Dscam1⁰⁵⁵¹⁸/Dscam[+], fra⁴ has Bolwig nerve phenotype

(Andrews et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Dscam1⁰⁵⁵¹⁸, robo1¹ double heterozygotes show a similar decrease in filopodial and lamellopodial activities compared to either single heterozygote conditions, and show no significant changes in migration velocity compared to wild-type controls.

(Raza and Jacobs, 2016)

Dscam1⁰⁵⁵¹⁸ DL1 projection neuron mutant clones generated in a TBCD¹/+ background have an increased number of dendrites that spill over to the outside of the antennal lobe compared to wild type clones in a TBCD¹/+ mutant background. The loss of the dorsal branch seen in Dscam1⁰⁵⁵¹⁸ mutant clones alone is not enhanced in a TBCD¹ mutant background.

(Okumura et al., 2015)

Dscam⁰⁵⁵¹⁸ fra⁴ double mutants show a significant increase in apoptosis in the thoracic and abdominal segments of the ventral nerve cord compared to controls.

(Newquist et al., 2013)

67% of Dscam⁰⁵⁵¹⁸/Dscam⁰⁵⁵¹⁸ fra⁴/fra⁴ embryos show defects in axon guidance in the Bolwig's nerve.

The penetrance of the axon guidance defect phenotype that is seen in the Bolwig's nerve of Dscam⁰⁵⁵¹⁸/Dscam⁰⁵⁵¹⁸ fra⁴/fra⁴ double mutant embryos is not increased by addition of Dscam3^c02826/Dscam3^c02826.

22.5% of Dscam⁰⁵⁵¹⁸/+ ; fra⁴/+ embryos show defects in axon guidance in the Bolwig's nerve.

38% of Df(1)NP5/+ ; Dscam⁰⁵⁵¹⁸/+ embryos show defects in axon guidance in the Bolwig's nerve.

34% of Df(1)NetAB^Δ/+ ; Dscam⁰⁵⁵¹⁸/+ embryos show defects in axon guidance in the Bolwig's nerve.

84% of segments fail to separate the anterior and posterior commissures correctly in Dscam⁰⁵⁵¹⁸ Dscam3^c02826 embryos.

Dscam⁰⁵⁵¹⁸ fra⁴ embryos show defects in the commissures of the central nervous system; 9% of anterior commissures are absent, 24% of anterior commissures are thin, 5% of posterior commissures are absent and 39% of posterior commissures are thin. 51% of segments fail to separate the anterior and posterior commissures correctly.

Dscam⁰⁵⁵¹⁸ Abl⁴ embryos show defects in the commissures of the central nervous system; 23% of anterior commissures are absent, 33% of anterior commissures are thin, 65% of posterior commissures are absent and 26% of posterior commissures are thin. 19% of segments fail to separate the anterior and posterior commissures correctly.

Dscam⁰⁵⁵¹⁸ fra⁴ Dscam3^c02826 embryos show defects in the commissures of the central nervous system; 39% of anterior commissures are absent, 51% of anterior commissures are thin, 36% of posterior commissures are absent and 55% of posterior commissures are thin. 5% of segments fail to separate the anterior and posterior commissures correctly.

Dscam⁰⁵⁵¹⁸ fra⁴ Abl⁴ embryos show defects in the commissures of the central nervous system; 98% of anterior commissures are absent, 2% of anterior commissures are thin and 100% of posterior commissures are absent.

The severity of the SP1 axon midline crossing defects seen in fra^unspecified embryos is enhanced in fra^unspecified Dscam⁰⁵⁵¹⁸ Dscam3^c02826 triple mutants.

37% of EG axon bundles are thin or absent in Dscam⁰⁵⁵¹⁸ fra^unspecified mutant embryos.

(Andrews et al., 2008)

About 42% of Dscam⁰⁵⁵¹⁸/dock³ embryos exhibit some Bolwig's nerve targeting defects. About 38% of Dscam⁰⁵⁵¹⁸/Pak⁶ embryos exhibit some Bolwig's nerve targeting defects.

(Schmucker et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Dscam1¹⁸

(Wang et al., 2002)

Rescued by

Dscam1⁰⁵⁵¹⁸ is rescued by Dscam1^+t73.3

(Kim et al., 2013)

Comments

The dramatic reduction in presynaptic arbor growth in Dscam1⁰⁵⁵¹⁸ mutants is rescued by expression of Dscam1^+t73.3.

(Kim et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

84237

P{PZ}Dscam1⁰⁵⁵¹⁸ robo1⁴/SM5

11412

P{PZ}Dscam1⁰⁵⁵¹⁸ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

(BDGP Project Members, 1994-1999)

Comments

Complements: l(2)43Bb^04614a. Complements: cos^k16101. Complements: cos^k16122.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

Dscam1⁰⁵⁵¹⁸

(Liu et al., 2024, Clarembaux-Badell et al., 2022)

Dscam1^P

(Alavi et al., 2016)

Dscam⁰⁵⁵¹⁸

Dscam^P1

(Okumura et al., 2015, Kim et al., 2013, Zhu et al., 2006, Wang et al., 2004, Wang et al., 2002)

Dscam^P

(Andrews et al., 2008)

Dscam^PZ5518

(Shin and Diantonio, 2011)

Dscam^p

(Schmucker et al., 2000)

dscam1^p

(Raza and Jacobs, 2016)

dscam^P1

(Palavalli et al., 2021)

l(2)05518

(Spradling et al., 1999)

l(2)05518⁰⁵⁵¹⁸

l(2)43Bc⁰⁵⁵¹⁸

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (20)

Report Sections

Open Close