FlyBase Allele Report: Dmel\pbl[09645]

General Information

Symbol

Dmel\pbl⁰⁹⁶⁴⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0009614

Feature type

allele

Associated gene

Dmel\pbl

Associated Insertion(s)

P{PZ}pbl⁰⁹⁶⁴⁵

Carried in Construct

Also Known As

l(3)09645

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}pbl⁰⁹⁶⁴⁵

Cytology

Description

P{PZ} insertion 492bp upstream of the AUG start codon of the 1A cDNA.

(Prokopenko et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,911,911..7,911,911 [+]

(Bowman et al., 2000, Prokopenko et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Jeong et al., 2012)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die during embryonic stage | recessive

(Kraut et al., 2001)

some die during embryonic stage | recessive

(Kraut et al., 2001)

Phenotype Manifest In

larval intersegmental nerve

(Jeong et al., 2012)

larval intersegmental nerve branch ISNb of A1-7

(Jeong et al., 2012)

larval segmental nerve branch SNa of A1-7

(Jeong et al., 2012)

Detailed Description

Statement

Reference

98% of hemisegments show ISNb guidance defects in homozygous embryos. The ISNb axons often fail to defasciculate from one another, resulting in an abnormally thick morphology and a failure to reach their muscle targets. In addition, ISNb axons that follow aberrant pathways and fasciculate ectopically with adjacent axons (including the transverse nerve) are seen.

90% of hemisegments show defects in the SNa axon in homozygous embryos. The dorsal or lateral SNa branches are often missing.

ISN axons fail to target the correct muscles in homozygous embryos. The first or second branches often extend dorsally beyond their correct muscle fields. Occasionally mutant ISN axons cross the segment boundary and fasciculate with adjacent ISN axons.

(Jeong et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

pbl[+]/pbl⁰⁹⁶⁴⁵ is a non-enhancer of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

NOT Suppressor of

Statement

Reference

pbl[+]/pbl⁰⁹⁶⁴⁵ is a non-suppressor of abnormal eye color phenotype of Hsap\MECP2^R294X.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

pbl[+]/pbl⁰⁹⁶⁴⁵ is a non-enhancer of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Suppressor of

Statement

Reference

pbl[+]/pbl⁰⁹⁶⁴⁵ is a suppressor of eye phenotype of Hsap\MECP2^UAS.FL, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

NOT Suppressor of

Statement

Reference

pbl[+]/pbl⁰⁹⁶⁴⁵ is a non-suppressor of eye phenotype of Hsap\MECP2^Δ166.UAS, Scer\GAL4^GMR.PU

(Cukier et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pbl^S008320

(Prokopenko et al., 2000)

pbl^S054203a

(Prokopenko et al., 2000)

Partially rescued by

pbl⁰⁹⁶⁴⁵ is partially rescued by Scer\GAL4^sca-537.4/pbl^UAS.N.Tag:HA

(Jeong et al., 2012)

Not rescued by

pbl⁰⁹⁶⁴⁵ is not rescued by Scer\GAL4^how-24B/pbl^UAS.N.Tag:HA

(Jeong et al., 2012)

Comments

Expression of pbl^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^how-24B fails to rescue the motor axon pathfinding defects seen in pbl⁰⁹⁶⁴⁵ embryos.

Expression of pbl^{Scer\UAS.N.T:Ivir\HA1} under the control of Scer\GAL4^sca-537.4 partially but significantly rescues the pathfinding defects in the ISNb and SNa, but not the ISN, motor axon pathways in pbl⁰⁹⁶⁴⁵ embryos.

(Jeong et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11737

P{PZ}pbl⁰⁹⁶⁴⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Kyoto

108876

P{PZ}pbl⁰⁹⁶⁴⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin