FlyBase Allele Report: Dmel\mus312[D1]

General Information

Symbol

Dmel\mus312^D1

Species

D. melanogaster

Name

FlyBase ID

FBal0012668

Feature type

allele

Associated gene

Dmel\mus312

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Boyd et al., 1981)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q226term.

(Yildiz et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:7,330,403..7,330,403 [+]

Nucleotide change:

C7330403T

Amino acid change:

Q226term | mus312-PA; Q226term | mus312-PD; Q226term | mus312-PE

Reported amino acid change:

Q226term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Yildiz et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

presumptive oocyte

(Joyce and McKim, 2009)

Detailed Description

Statement

Reference

X-ray irradiation of wild-type third-instar larvae induces cell cycle arrest within 1 hour. In contrast, X-ray irradiation of mus312^Z1973/mus312^D1 mutant larvae fails to induce cell cycle arrest.

X-ray irradiation of wild-type third-instar larvae induces cell cycle arrest within 1 hour. In contrast, X-ray irradiation of mus312^Z3997/mus312^D1 mutant larvae fails to induce cell cycle arrest.

(Kondo and Perrimon, 2011)

mus312^D1 fails to complement a quantitative trait locus (QTL) for aggressive behaviour; the mus312^D1/QTL genotype shows lower levels of aggression than the mus312^D1/wild-type genotype.

(Edwards and Mackay, 2009)

Two pro-oocytes are visible in 75.0% of region 3 cysts in homozygous females (as assayed by c(3)G staining) compared to a frequency of 9.5% in wild type.

(Joyce and McKim, 2009)

mus312^D1/mus312^D2 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32^oC or 38^oC.

(Wei and Rong, 2007)

mus312^D1 homozygotes much lower meiotic crossover frequency than wild-type flies. Survival of mus312^D1/mus312^Z1973 adults treated with DNA damaging agent Nitrogen mustard, is significantly reduced compared to wild-type. mus312^Z1973/mus312^D1 and mus312^Z3997/mus312^D1 animals have X chromosome non-disjunction rates of 24%.

(Yildiz et al., 2002)

Flies are sensitive to X rays.

(Oliveri et al., 1990)

Sensitive to 0.004% HN2 and 0.125% methyl methanesulfonate. Homozygous females show an increased frequency of nondisjunction.

(Boyd et al., 1981)

Larval survival hypersensitive to exposure to X rays (Oliveri, Harris and Boyd, 1990), methyl methanesulfonate and nitrogen mustard. Reduces meiotic recombination (Green, 1981). homozygous fertile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has lethal | pupal stage phenotype, non-suppressible by spn-A^unspecified

(Andersen et al., 2011)

Suppressor of

Statement

Reference

mus312^D1/mus312^Z1973 is a suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

Other

Statement

Reference

WRNexo^Δ, mus312^D1 has lethal - all die during pharate adult stage phenotype

(Bolterstein et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus312^D1/mus312^Z1973 has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mus312^D1/mus312^Z1973, mus81^Nhe has viable phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973 has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has lethal | pupal stage phenotype

(Andersen et al., 2011, Andersen et al., 2009)

Blm^N2/Blm^D2, mus312^D1/mus312^Z1973 has lethal | pupal stage phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has melanotic mass phenotype | third instar larval stage phenotype

(Andersen et al., 2009)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal | pupal stage phenotype

(Andersen et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has condensed chromosome phenotype, suppressible by spn-A^unspecified

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has salivary gland imaginal ring phenotype, suppressible by spn-A^unspecified

(Andersen et al., 2011)

NOT suppressed by

Statement

Reference

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has larval brain phenotype, non-suppressible by spn-A^unspecified

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has imaginal disc | larval stage phenotype, non-suppressible by spn-A^unspecified

(Andersen et al., 2011)

Other

Statement

Reference

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has larval brain phenotype

(Andersen et al., 2011, Andersen et al., 2009)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has salivary gland imaginal ring phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has imaginal disc | larval stage phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has condensed chromosome phenotype

(Andersen et al., 2011)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973 has imaginal disc | third instar larval stage phenotype

(Andersen et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

WRNexo^Δ/WRNexo^Δ mus312^D1/mus312^D1 double mutants survive to pharate adult stage.

(Bolterstein et al., 2014)

Spontaneous mitotic crossovers are completely absent in Fancm⁰⁶⁹³/Df(3R)ED6058 animals that are also mutant for mus312^D1/mus312^Z1973.

(Kuo et al., 2014)

mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 double mutant larvae have small brains, lack imaginal discs and have a reduced number of salivary gland imaginal ring cells. The nuclei of the remaining salivary gland imaginal ring cells appear larger than normal. Mitotic neuroblasts in these larvae show extreme genome instability; no mitotic nuclei with completely intact chromosomes are detected and approximately one-third show polyploidy.

The addition of spn-A^unspecified suppresses the the chromosome breakage and polyploidy phenotypes seen in mitotic neuroblasts of mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 larvae, and also suppresses the defects in the salivary gland imaginal ring cells seen in the mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 double mutants. However, addition of spn-A^unspecified does not suppress the pupal lethality, small brains and lack of imaginal discs seen in the mus309^N1/mus309^D2 mus312^D1/mus312^Z1973 double mutants.

mus309^D2/mus309^N2 mus312^D1/mus312^Z1973 double mutant larvae generally lack imaginal discs (although small, severely underdeveloped discs are sometimes seen in third instar larvae).

(Andersen et al., 2011)

mus312^D1/mus312^Z1973, mus309^D2/mus309^N1 double mutants are inviable, dying after pupariation. Third-instar larvae have melanotic tumors and lack larval imaginal discs. Larval brains of double mutants are greatly reduced in size.

The spn-A³/spn-A^093A transheterozygous condition does not suppress the lethality of mus312^D1/mus312^Z1973, mus309^D2/mus309^N1 double mutants.

(Andersen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

329

mus312^D1

Notes on Origin

Discoverer

Boyd.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mus(3)312^D1

(Olsen and Green, 1982)

mus312^D1

(Goupil et al., 2022, Carvajal-Garcia et al., 2021, Bolterstein et al., 2014, Kuo et al., 2014, Andersen et al., 2011, Kondo and Perrimon, 2011, Andersen et al., 2009, Edwards and Mackay, 2009, Joyce and McKim, 2009, Wei and Rong, 2007)

Name Synonyms

Secondary FlyBase IDs

References (17)

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