FlyBase Allele Report: Dmel\Ubx[bx-3]

General Information

Symbol

Dmel\Ubx^bx-3

Species

D. melanogaster

Name

FlyBase ID

FBal0017517

Feature type

allele

Associated gene

Dmel\Ubx

Associated Insertion(s)

gypsy{}Ubx^bx-3

Carried in Construct

Also Known As

bx³

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Lindsley and Zimm, 1992, Peifer and Bender, 1986, Bender et al., 1983)

Progenitor genotype

Associated Insertion(s)

gypsy{}Ubx^bx-3

Cytology

Polytene chromosomes normal.

(Castelli-Gair and Garcia-Bellido, 1990)

Description

Insertion of a gypsy element at map position -57kb. The direction of transcription of the gypsy element is in the opposite direction to Ubx transcription.

(Bender et al., 1983)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Muralidhar and Thomas, 1993)

viable

(Bischoff et al., 2009)

visible

(Schneiderman et al., 1993)

visible | homeotic

(Bender et al., 1983)

visible | homeotic (with Df(3R)P2)

(Rivlin et al., 2001)

visible | homeotic | recessive

(Cole and Palka, 1982)

Phenotype Manifest In

abdominal segment 1

(Castelli-Gair et al., 1992)

adult mesothoracic segment anterior compartment | ectopic (with Df(3R)P2)

(Rivlin et al., 2001)

adult metathoracic segment

(Schneiderman et al., 1993)

adult metathoracic segment anterior compartment (with Df(3R)P2)

(Rivlin et al., 2001)

adult muscle system & abdominal segment 1

(Roy and VijayRaghavan, 1997)

direct flight muscle cell | ectopic (with Df(3R)P2)

(Rivlin et al., 2001)

dorsal longitudinal indirect flight muscle cell | ectopic (with Df(3R)P2)

(Rivlin et al., 2001)

ectoderm & metathoracic segment

(Fernandes et al., 1994)

giant fiber neuron

(Schneiderman et al., 1993)

haltere

(LaJeunesse and Shearn, 1995, Lindsley and Zimm, 1992, Peifer and Bender, 1986, Bender et al., 1983, Lewis, 1959)

haltere | anterior

(Cole and Palka, 1982)

haltere disc anterior compartment (with Ubx^6.28)

(de Navas et al., 2006)

haltere disc anterior compartment (with Ubx¹³⁰)

(de Navas et al., 2006)

katepimeron

(Peifer and Bender, 1986)

lateral oblique dorsal muscle cell | ectopic (with Df(3R)P2)

(Rivlin et al., 2001)

leg

(Kuzin et al., 1997)

mesothoracic tergotrochanter muscle cell

(Schneiderman et al., 1993)

metathoracic leg

(LaJeunesse and Shearn, 1995, Bender et al., 1983)

metathoracic leg | anterior

(Peifer and Bender, 1986)

metathoracic leg anterior compartment

(Minana and Garcia-Bellido, 1982)

metathoracic segment

(Castelli-Gair et al., 1992)

metathoracic segment | dorsal

(Peifer and Bender, 1986)

myoblast

(Fernandes et al., 1994)

tergosternal muscle cell | ectopic (with Df(3R)P2)

(Rivlin et al., 2001)

Detailed Description

Statement

Reference

Ubx^LDN/Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1 adults show transformation of the distal part of the haltere (the capitellum) into the wing; the haltere is bigger than normal and has bristles on the margins and veins on the surface.

(de Navas et al., 2011)

The anterior compartment is increased in size in Ubx¹³⁰/Ubx^bx-3 and Ubx^6.28/Ubx^bx-3 haltere discs.

(de Navas et al., 2006)

The haltere is partially transformed to a wing in Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^LDN flies.

(de Navas et al., 2006)

In Ubx^bx-3 Ubx^pbx-1 Ubx^6.28 L3 larvae, tracheal metamere 3 is transformed into tracheal metamere 2. Tracheal metamere 2 appears normal.

(Guha and Kornberg, 2005)

Ubx^bx-3/Df(3R)P2 flies show transformation of the anterior compartment of T3 (T3a) into the anterior compartment of T2 (T2a). The homeotically transformed T3 segment can contain DVM-I, DVM-III, DLM and DFM muscles. The homeotic DVM-I muscle is composed of one fibre and the diameter of the homeotic DVM-I fibre is less than that seen in T2. Homeotic DVM-II muscles are not seen. Ubx^abx-1 Ubx^bx-3 Ubx^61d Ubx^pbx-1/Df(3R)P2 mutants show transformation of T3 into T2 resulting in the replacement of halteres with a second set of wings. The homeotically transformed T3 segment resembles T2 in size. The homeotically transformed T3 segment can contain homeotic DVM-I, DVM-II, DVM-III, DLM and DFM muscles.

(Rivlin et al., 2001)

Ubx^abx-1, Ubx^bx-3 and Ubx^pbx-1 triple mutants cause muscle patterning defects in A1 of pupae, transforming them towards a T3 thoracic identity.

(Roy and VijayRaghavan, 1997)

Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1 triple mutant adults have four wings.

(Roy et al., 1997)

In Ubx^abx-1 Ubx^pbx-1 Ubx^bx-3 triple mutant flies the T3 ectoderm transforms toward T2 identity. Transformation changes the pattern of myoblasts on the transformed haltere disc and their pattern of migration, but indirect flight muscles do not form as there are no larval templates. The haltere disc is almost completely transformed into a wing disc. Adults have two pairs of wings, but the homeotically transformed T3 has only rudimentary indirect flight muscles.

(Fernandes et al., 1994)

The giant fibre (GF) of Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Df(3R)P2 mutants makes a midline tuft and extends a lateral process to T2 and T3 segment. In the presence of ben¹ the GF is capable of extending a process into the T3 segment but no lateral processes from the midline.

(Muralidhar and Thomas, 1993)

Flies triply mutant for Ubx^abx-1, Ubx^bx-3 and Ubx^pbx-1 over Df(3R)P2 have a transformed metathorax including neural elements and jump muscle, that function in the escape-jump response within that segment. Corresponding transformation of the flight muscles is infrequent.

(Schneiderman et al., 1993)

Ubx¹/Ubx^bx-3 transheterozygotes are viable, with the following transformations in the adult: T3a to T2a, T3p to T2p, and A1a to T3a.

(Castelli-Gair et al., 1992)

Viability low but can be maintained homozygous. Halteres enlarged in heterozygote. Interactions with other Ubx 'bx' alleles described by Lewis (1951, 1955, 1963). Almost completely suppressed by su(Hw)².

(Lindsley and Zimm, 1992)

Does not affect the frequency of the trx bithorax-variegated phenotype in heterozygous combination with Df(3R)red-P52.

(Castelli-Gair and Garcia-Bellido, 1990)

Ubx^bx-3/Ubx¹³⁰ flies show a strong transformation of anterior haltere to anterior wing. The proximal metathorax is transformed to proximal mesothorax.

(Casanova et al., 1988)

Homozygotes and transheterozygotes of Ubx alleles demonstrate a significant enhancement of the bx phenotype when in combination with E(bx)². Haltere to winglike and metanotum to mesonotumlike transformations are enhanced.

(Bhosekar and Babu, 1987)

Different genetic combinations of Pc alleles and Ubx alleles suggest that the products of the bx region of Ubx are responsible for the "cbx" phenotype of the Pc mutations and in the absence of Pc the overexpression of bx gene products suppresses the pbx insufficiency phenotype in the posterior segments.

(Capdevila et al., 1986)

Haltere are transformed to wing and are 20-25% of wing size, dorsal tissue of T3 is incompletely transformed to metanotum, hypopleural plates are incompletely transformed to sternopleural plates, 8-10 hypopleural bristles are still present and the anterior third leg displays a strong transformation to anterior second leg, as seen by the bristle pattern. Phenotype can be suppressed by su(Hw) mutations.

(Peifer and Bender, 1986)

Ubx^bx-3 shows the "ppx" transformation at 17^oC: ventral regions of T2p and T3p are affected.

(Casanova et al., 1985)

Flies have normal transversal fascicles in the adult CNS.

(Teugels and Ghysen, 1985)

Ubx^bx-3 Ubx^bxd-106/Df(3R)P115 clones in T3 show a transformation to T2 in legs and halteres. Clones in the posterior compartments of legs in T2 or T3 do not show transformation into prothorax.

(Sanchez-Herrero and Morata, 1984)

Strong transformation of anterior third thoracic segment to second thoracic segment.

(Bender et al., 1983)

Homozygotes show a variable transformation of anterior haltere to wing. The transformed appendage is often misshapen, the most proximal sensilla fields are the most perfectly transformed, whereas the more distal fields often remain haltere-like. Dorsally on the transformed appendage, the ANWP and Teg. sensilla always appear normal, field d.Rad.A occasionally shows extra type-2 sensilla, the d.Rad.B and d.Rad.C fields often have fewer sensilla than normal, and the most distal sensilla have rudimentary sockets rather than smooth depressions. Sensilla on the medial radius are abnormal; d.Rad.D consists of aberrant type-5 sensilla not representative of wing or haltere, the d.Rad.E sensilla appear wing-like but crowded, and the dHCV and GSR sensilla of the distal radius are surrounded by extra sensilla of varying numbers and morphology. Ventrally, the v.Rad.A sensilla show normal morphology, although a fifth sensillum is often seen, the v.Rad.B sensilla are usually normal although they may be absent and the v.Rad.C field is sometimes replaced by fused, pedicellar-type sensilla which extend out over the more distal regions of the ventral radius. Ubx^bx-3 Ubx^pbx-1 flies show a transformation of haltere to wing. The transformed appendage is smaller than wild-type, the cuticle is often folded, and the veins are often obscure. The phenotype of the sensilla is similar to Ubx^bx-3 homozygotes, except that the homeotic transformation in the proximal radius region is more complete, and d.Rad.D contains sensilla resembling, but not identical to, the pedicellar sensilla of the haltere. Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1 flies have a transformation of haltere to wing. The homeotic appendage is somewhat smaller than a normal wing, but usually has the full wild-type complement of wing sensilla, with each type located in the normal position and having normal morphology. The only exception is an occasional decrease in the number of sensilla in fields d.Rad.B and d.Rad.C, and rarely, one or a few extra haltere pedicellar bristles.

(Cole and Palka, 1982)

Hemizygous Ubx^bx-3/Df(3R)P115 tissue shows a transformation of the metathoracic segment (both dorsal and ventral discs) to the mesothoracic segment in gynandromorphs. This transformation is restricted to the anterior compartment.

(Minana and Garcia-Bellido, 1982)

The frequency of variegated bithorax transformations seen in Df(3R)red-P52 heterozygotes is not increased in flies also heterozygous for Ubx^bx-3.

(Capdevila and Garcia-Bellido, 1981)

Heterozygotes with Ubx^61d has halteres partially wing-like but lack conversion of metanotum towards mesonotum.

(Lewis, 1980)

The posterior portion of the halteres shows a slight wing-like modification in Ubx^bx-3/Ubx^pbx-1 flies.

(Lewis, 1959)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ubx^bx-3 has viable phenotype, suppressible by xen[+]/Asx^xen-72-3

(Bischoff et al., 2009)

Other

Statement

Reference

Ubx^bx-3/Ubx^bxd-51j, ss^a has visible phenotype

(Kuzin et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ubx^bx-3 has phenotype, enhanceable by ash1²⁸

(LaJeunesse and Shearn, 1995)

Ubx^bx-3 has phenotype, enhanceable by ash2¹⁸

(LaJeunesse and Shearn, 1995)

Ubx^bx-3 has phenotype, enhanceable by E(bx)¹

(Lindsley and Zimm, 1992)

Ubx^bx-3 has haltere phenotype, enhanceable by z^ae(bx)

(Lewis, 1959)

Suppressed by

Statement

Reference

Ubx¹³⁰/Ubx^bx-3 has haltere disc anterior compartment phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/tkv^UAS.cLa

(de Navas et al., 2006)

Ubx^bx-3/Ubx^6.28 has haltere disc anterior compartment phenotype, suppressible | partially by Scer\GAL4^ptc-559.1/tkv^UAS.cLa

(de Navas et al., 2006)

Ubx^bx-3 has phenotype, suppressible by su(Hw)^unspecified

(Bender et al., 1983)

Ubx^bx-3 has phenotype, suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Other

Statement

Reference

Ubx^bx-3/Ubx^bxd-51j, ss^a has leg phenotype

(Kuzin et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Asx^xen-72-3 in a homozygous Ubx^bx-3 mutant background causes lethality at the third instar larval stage.

(Bischoff et al., 2009)

The increased size of the anterior compartment that is seen in the haltere discs of Ubx^6.28/Ubx^bx-3 animals is partially suppressed by expression of tkv^Scer\UAS.cLa under the control of Scer\GAL4^ptc-559.1.

(de Navas et al., 2006)

Expression of abd-A^{1dB5.Scer\UAS} results in an almost complete rescue of the transformed haltere phenotype of Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^LDN flies. The P{GawB}Ubx^LDN insertion within the Ubx^LDN flies drives expresssion of abd-A^{1dB5.Scer\UAS}.

(de Navas et al., 2006)

ss^a Ubx^bx-3 Ubx^bxd-51j hemizygotes do not emerge as imagos. Dissection of late pupae reveals flies have four pairs of legs (Ubx^bx-3 Ubx^bxd-51j phenotype) and the fourth pair of legs exhibit severe disruption of segmentation and an improvement in the segmentation of the third pair.

(Kuzin et al., 1997)

Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^lac1 combination transforms the metathorax into mesothorax (including transformation of halteres into wings). This phenotype can be rescued by Scer\GAL4^Bx-MS1096 induced expression abd-A. Ectopic expression of Abd-B allows partial rescue.

(Casares et al., 1996)

Double heterozygotes for ash1²⁸ at 27^oC exhibit haltere to wing transformation. No transformation phenotype is seen at 20^oC. Double heterozygotes for ash2¹⁸ at 27^oC exhibit haltere to wing transformation and mild third to second leg transformation.

(LaJeunesse and Shearn, 1995)

The Ubx^bx-3/Ubx^pbx-1 phenotype is enhanced by z^ae(bx).

(Lewis, 1959)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

Ubx^abx-1

(Lewis, 1980)

Comments

The partial transformation of haltere into wing which is seen in Ubx^LDN/Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1 flies is strongly suppressed by expression of either Ubx^Scer\UAS.cCa or Ubx^{IIa.Scer\UAS.cRa} under the control of Scer\GAL4^Ubx-LDN.

The partial transformation of haltere into wing which is seen in Ubx^LDN/Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1 flies is partially suppressed by expression of one copy of Ubx^{IVa.Scer\UAS.cRa} under the control of Scer\GAL4^Ubx-LDN and is almost completely suppressed by expression of two copies of Ubx^{IVa.Scer\UAS.cRa} under the control of Scer\GAL4^Ubx-LDN.

(de Navas et al., 2011)

(Casares et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (12)

Bloomington

3419

Ubx^bx-3/T(2;3)ap^Xa, ap^Xa

3457

Sb^sbd-2 Ubx^bx-3 Ubx^pbx-1/TM1

3485

Df(3R)red-P52, Sb^sbd-2 Ubx^bx-3 Ubx^pbx-1/TM1

305

Ubx^bx-3 Abd-B^Uab-1/T(2;3)ap^Xa, ap^Xa

3414

Ubx^bx-3 Ubx^bxd-1 Ubx^pbx-1/T(2;3)ap^Xa, ap^Xa

Kyoto

106812

Ubx^abx-1 Ubx^bx-3 Ubx^pbx-1/Ubx^61d

107061

Df(3R)red-P52, Sb^sbd-2 Ubx^bx-3 Ubx^pbx-1/TM1

105847

Ubx^bx-3 Abd-B^Uab-1/T(2;3)ap^Xa, ap^Xa

106303

In(3LR)bxd¹⁰⁶, Sb^sbd-2 Ubx^bx-3 Ubx^bxd-106 / Tp(3;Y)J158

107066

Dp(3;3)bxd¹⁰⁰, red¹ Sb^sbd-2 Ubx^bx-3 abd-A^Hab-1/TM1

101566

Ubx^abx-1 Ubx^bx-3 Ubx^61d Ubx^pbx-1 / TM1, Me¹ kni^ri-1 Sb^sbd-l

106321

Dp(3;3)S2, ru¹ Hn^r3 hry² app¹ cu¹ kar¹/In(3LR)HR33^Lbxd^106R, Sb^sbd-2 Ubx^bx-3

Notes on Origin

Discoverer

Stern.

(Peifer and Bender, 1986)

Stern, 2 Feb. 1925.

(Lindsley and Zimm, 1992)

The original Ubx^bx-3 chromosome had two insertions in Ubx, a gypsy insertion at -57kb and a Doc insertion at -53kb. Only the gypsy element is responsible for the Ubx^bx-3 mutant phenotype: a recombinant chromosome without the Doc insertion has the same phenotype as the original chromosome, and loss of the gypsy insertion results in reversion of the Ubx^bx-3 mutant phenotype.

(Bender et al., 1983)

Comments

The phenotype of a number of combinations of Ubx mutations has been investigated to determine the role of the bxd regulatory region in the modulation of Ubx expression.

(Castelli-Gair et al., 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Ubx^bx-3

Ubx^bx3

(Bischoff et al., 2009)

bx-3

(O'Keefe et al., 2005)

bx3

(de Navas et al., 2011)

bx³

(Simoes da Silva et al., 2019, de Navas et al., 2006, de Navas et al., 2006, Guha and Kornberg, 2005, Bajpai et al., 2004, Crow and Bender, 2004, Duncan, 2002, Duncan and Montgomery, 2002, Rivlin et al., 2001, Lewis, 1998, Casares et al., 1997, Kuzin et al., 1997, Rivlin et al., 1997, Roy and VijayRaghavan, 1997, Roy et al., 1997, Casares et al., 1996, Laney and Biggin, 1996, Martinez-Laborda et al., 1996, LaJeunesse and Shearn, 1995, Shashidhara et al., 1995, Camprodon and Castelli-Gair, 1994, Fernandes et al., 1994, Muralidhar and Thomas, 1993, Schneiderman et al., 1993, Cash et al., 1992, Castelli-Gair et al., 1992, Castelli-Gair et al., 1992, Lindsley and Zimm, 1992, Qian et al., 1991, Castelli-Gair and Garcia-Bellido, 1990, Castelli-Gair et al., 1990, Little et al., 1990, Mathog, 1990, Micol et al., 1990, Botas et al., 1988, Casanova et al., 1988, Babu et al., 1987, Bhosekar and Babu, 1987, Weinzierl et al., 1987, Capdevila et al., 1986, Peifer and Bender, 1986, Casanova et al., 1985, Teugels and Ghysen, 1985, Sanchez-Herrero and Morata, 1984, Bender et al., 1983, Cole and Palka, 1982, Kerridge and Morata, 1982, Minana and Garcia-Bellido, 1982, Capdevila and Garcia-Bellido, 1981, Lewis, 1980, Lewis, 1959)

Name Synonyms

Secondary FlyBase IDs

References (62)

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