FlyBase Allele Report: Dmel\gish[04895]

General Information

Symbol

Dmel\gish⁰⁴⁸⁹⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0028445

Feature type

allele

Associated gene

Dmel\gish

Associated Insertion(s)

P{PZ}gish⁰⁴⁸⁹⁵

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Castrillon et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}gish⁰⁴⁸⁹⁵

Cytology

Description

Insertion in the second exon.

(Tan et al., 2010)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:16,274,943..16,274,943 [+]

(Howlett et al., 2013, Spradling et al., 1999, BDGP Project Members, 1994-1999, Castrillon et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Howlett et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | dominant

(Tan et al., 2010)

abnormal size | adult stage

(Chen et al., 2017)

decreased cell number | adult stage | germline clone | progressive

(Chen et al., 2017)

decreased cell number | adult stage | progressive

(Chen et al., 2017)

decreased cell number | adult stage | progressive (with gish^KG03891)

(Chen et al., 2017)

decreased cell number | adult stage | progressive (with gish^MI08417)

(Chen et al., 2017)

lethal | recessive

(Howlett et al., 2013)

male sterile

(Tan et al., 2010)

male sterile | recessive

(Spradling et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

gish⁰⁴⁸⁹⁵ homozygous adult males have small testes and along with the gish⁰⁴⁸⁹⁵/gish^MI08417 or gish⁰⁴⁸⁹⁵/gish^KG03891 transheterozygous mutants show decreased average number of germline stem cells in the testes already at post-eclosion day 1 and as the flies age the GSCs continue to be lost at a rapid rate, this however is not due to excessive apoptosis and neither do the mutant GSCs lose their adhesion to the hub cells as shown in gish⁰⁴⁸⁹⁵/gish^MI08417 males. No significant difference in the number of GSCs is observed in gish⁰⁴⁸⁹⁵ heterozygotes, as compared to wild-type controls.

The germline stem cells in the heat-shock induced gish⁰⁴⁸⁹⁵ mutant germline clones in testes of adult male flies are rapidly lost as the flies age.

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵/+ mutant flies exhibit a seizure-resistant phenotype, exhibiting a seizure threshold of about twice that of wild type. No other phenotypes are apparent; electrophysiology, behavior and morphology are all wild type.

(Howlett et al., 2013)

Heterozygotes show a significant impairment in 30 minute and 3 hour memory after olfactory conditioning compared to wild-type flies, but do not show significant impairment in 3 minute memory.

Heterozygotes and homozygotes show normal shock and odour avoidance.

(Tan et al., 2010)

The nuclei of onion stage spermatids are scattered over the cyst in its basal third in homozygous males (the nuclei form a bundle at this stage in wild-type males). The actin cones also fail to form a bundle in the mutant spermatids. In late individualisation stage spermatids, the normal association between mitochondrial derivative and axoneme is lost in mutant males. In addition, although the major mitochondrial derivative has a horseshoe-shaped morphology characteristic of this stage, the minor mitochondrial derivative displays a morphology characteristic of the early individualisation stage.

(Nerusheva et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

gish[+]/gish⁰⁴⁸⁹⁵ is a suppressor of bang sensitive | semidominant phenotype of para^bss1

(Howlett et al., 2013)

NOT Suppressor of

Statement

Reference

gish[+]/gish⁰⁴⁸⁹⁵ is a non-suppressor of bang sensitive phenotype of para^bss1

(Howlett et al., 2013)

gish[+]/gish⁰⁴⁸⁹⁵ is a non-suppressor of bang sensitive phenotype of eas²

(Howlett et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The bang sensitive phenotype seen in para^bss1/+ mutant females can be partially suppressed with one copy of gish⁰⁴⁸⁹⁵. Fewer flies exhibit bang-sensitivity and the threshold for seizure-like activity is partially rescued. A gish⁰⁴⁸⁹⁵/+ background does not suppress the bang sensitive phenotypes in para^bss1/para^bss1 or para^bss1/Y mutant flies.

The bang sensitive phenotype seen in eas²/+ mutant females is not suppressed by one copy of gish⁰⁴⁸⁹⁵. The penetrance and threshold for seizure initiation are similar to in eas² mutants alone.

(Howlett et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

gish⁰⁴⁸⁹⁵/gish^MI08417 is rescued by gish^gish.F

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵/gish^MI08417 is rescued by gish^UASp.F/Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵/gish^KG03891 is rescued by gish^gish.F

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵/gish^KG03891 is rescued by gish^UASp.F/Scer\GAL4^{VP16.nanos.UTR}

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵ is rescued by gish^gish.F

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵ is rescued by gish^nanos.F

(Chen et al., 2017)

Not rescued by

gish⁰⁴⁸⁹⁵/gish^MI08417 is not rescued by gish^UASp.F/Scer\GAL4^C587

(Chen et al., 2017)

gish⁰⁴⁸⁹⁵/gish^KG03891 is not rescued by gish^UASp.F/Scer\GAL4^C587

(Chen et al., 2017)

Comments

The age-progressive loss of germline stem cells in adult male testes observed in gish⁰⁴⁸⁹⁵ homozygotes as well as gish⁰⁴⁸⁹⁵/gish^KG03891 or gish⁰⁴⁸⁹⁵/gish^MI08417 transheterozygotes is fully rescued by combination with either gish^gish.F. Similarly, combination with gish^nos.F also rescues the phenotype in gish⁰⁴⁸⁹⁵ homozygotes and both gish⁰⁴⁸⁹⁵/gish^KG03891 and gish⁰⁴⁸⁹⁵/gish^MI08417 mutants can be rescued by expression of gish^{Scer\UAS.P\T.F} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} (but not Scer\GAL4^C587).

(Chen et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11790

ry⁵⁰⁶ P{PZ}gish⁰⁴⁸⁹⁵/MKRS

Notes on Origin

Discoverer

A. Spradling.

Comments

Complements: l(3)05203⁰⁵²⁰³.

(BDGP Project Members, 1994-1999)

Complements Df(3R)sbd105, indicating that the insertion does not cause the male sterility, or has been mismapped.

(Kennison, 1995.6.21)

Precise excision of the P-element completely suppresses the bang-sensitive phenotype and restores viability, but does not appear to revert the male sterility phenotype.

(Howlett et al., 2013)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CKI-related⁰⁴⁸⁹⁵

Hrr25⁰⁴⁸⁹⁵

gish04895

(Howlett et al., 2013)

gish⁰⁴⁸⁹⁵

(Chen et al., 2017, Howlett et al., 2013, Tan et al., 2010, Nerusheva et al., 2009, Nerusheva et al., 2007, Rathke et al., 2007)

ms(3)04895

(Spradling et al., 1999)

ms(3)89B⁰⁴⁸⁹⁵

(Kennison, 1995.6.21, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

ms(3)ry89B

Name Synonyms

Secondary FlyBase IDs

References (11)

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