FlyBase Allele Report: Dmel\if[B2]

General Information

Symbol

Dmel\if^B2

Species

D. melanogaster

Name

FlyBase ID

FBal0031512

Feature type

allele

Associated gene

Dmel\if

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Bloor and Brown, 1998)

hypomorphic allele - genetic evidence

(Chanana et al., 2007, Brower, 1992)

loss of function allele

(Brabant and Brower, 1993)

Mutagen

ethyl methanesulfonate

(Bloor and Brown, 1998, Brabant and Brower, 1993, Brower, 1992)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Ayyub and Paranjape, 2002)

lethal | recessive

(Hoang and Chiba, 1998, Brower, 1992)

lethal - all die during embryonic stage

(Brower et al., 1995)

lethal - all die during embryonic stage | recessive

(Bloor and Brown, 1998, Brabant and Brower, 1993)

some die during embryonic stage

(Brower et al., 1995)

some die during embryonic stage | recessive

(Bloor and Brown, 1998, Brabant and Brower, 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult if^B2 homozygous mutant intestinal stem cell clones have similar maintenance 7 days and 14 days after clone induction compared to control clones.

(Lin et al., 2013)

Fewer haemocytes are observed migrating into the tail region in if^B2 mutant embryos. There is no decrease in migration to the dorsal vessel or ventral nerve cord in these animals.

(Siekhaus et al., 2010)

if^B2 mutant embryos develop a mild muscle detachment phenotype in several segments.

(Chanana et al., 2007)

When tested in the odour-induced jump-test, double heterozygotes of sws^olfE-x26 and if^B2 exhibit a reduced response to benzaldehyde, but not to iso-amyl acetate or ethyl acetate, compared to controls

(Ayyub and Paranjape, 2002)

Stage 14 oocytes carrying if^B2 somatic clones are much more rounded than wild-type.

(Bateman et al., 2001)

Embryos exhibit normal epidermis and resultant secreted cuticle, defects lie in internal tissues. Somatic muscle detach and round up. Gut morphogenesis is defective: anterior midgut does not become a slender tube and only two fat gastric caecae are formed. The ventral nerve cord does not fully condense.

(Bloor and Brown, 1998)

Homozygous embryos have an increased rate of axon errors in the central and peripheral nervous systems compared to wild-type.

(Hoang and Chiba, 1998)

Small clones (fewer than 150 cells) in the wing are often wild type or have a weak phenotype, even if on the ventral surface. mew^H7 if^B2 double mutant small clones display a morphological phenotype.

(Brabant et al., 1996)

Mitotic clones in the eye have normal morphology.

(Roote and Zusman, 1996)

Clones in the eye do not exhibit photoreceptor disorganisation.

(Brower et al., 1995)

Extreme defects in somatic muscle attachment sites and morphogenesis of the midgut in the embryo. Muscles pull away from attachment sites at the onset of contractions. Ventral nerve cord fails to shorten completely. There is no dorsal herniation defect. Clonal analysis demonstrated that if function is only required in the cells of the ventral wing surface.

(Brabant and Brower, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

if[+]/if^B2 is a non-enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

if[+]/if^B2 is a non-enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

if^B2 is a suppressor of visible phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

NOT Suppressor of

Statement

Reference

if[+]/if^B2 is a non-suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

if[+]/if^B2 is a non-suppressor of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

if^B2, p130CAS¹ has partially lethal - majority die | embryonic stage phenotype

(Tikhmyanova et al., 2010)

if^B2, sws^olfE-x26 has abnormal smell perception phenotype

(Ayyub and Paranjape, 2002)

if^B2, mys^olfC-x3 has abnormal smell perception phenotype

(Ayyub et al., 2000)

if^B2, mys^olfC-x10 has abnormal smell perception phenotype

(Ayyub et al., 2000)

if^B2, mys^olfC-x17 has abnormal smell perception phenotype

(Ayyub et al., 2000)

if^B2, mys^olfC-x5 has abnormal smell perception phenotype

(Ayyub et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

if^B2 has muscle attachment site phenotype, enhanceable by Tsp^Δ79

(Chanana et al., 2007)

if^B2 has muscle attachment site phenotype, enhanceable by Tsp[+]/Tsp^unspecified

(Chanana et al., 2007)

Enhancer of

Statement

Reference

if^B2 is an enhancer of muscle attachment site phenotype of Tsp^Δ79

(Chanana et al., 2007)

NOT Enhancer of

Statement

Reference

if[+]/if^B2 is a non-enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

if[+]/if^B2 is a non-enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

if^B2 is a suppressor of eye phenotype of H^UAS.cMa, Scer\GAL4^GMR.PF

(Müller et al., 2005)

NOT Suppressor of

Statement

Reference

if[+]/if^B2 is a non-suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

if[+]/if^B2 is a non-suppressor of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

Other

Statement

Reference

βTub85Dⁿ, if^B2 has presumptive embryonic salivary gland phenotype

(Jattani et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Approximately 56% of if^B2/+; p130CAS¹/p130CAS¹ double mutants emerge as adults.

(Tikhmyanova et al., 2010)

Approximately 30% of βTub85Dⁿ and if^B2 mutant embryos exhibit salivary gland migration defects.

(Jattani et al., 2009)

The mild muscle detachment phenotype of if^B2 mutant embryos is strongly enhanced, both with respect to the extent of detachment and penetrance in embryos that are also heterozygous for Tsp^unspecified. In these embryos, longitudinal, ventral as well as dorsal muscles are detached, a phenomenon not observed in homozygous Tsp^unspecified single mutant embryos.

if^B2/Y; Tsp^Δ79 double mutant embryos develop dramatic muscle pattern defects which is beyond an additive effect of the two individual mutant phenotypes.

(Chanana et al., 2007)

In combination with either mys^olfC-x3, mys^olfC-x5, mys^olfC-x10 or mys^olfC-x17, if^B2 flies show a reduced olfactory response to isoamyl acetate and benzaldehyde. In combination with mys^olfC-x5 or mys^olfC-x17 (but not mys^olfC-x10 or mys^olfC-x3), if^B2 flies show a reduced olfactory response to ethyl acetate.

(Ayyub et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

2176

106685

Notes on Origin

Discoverer

Brabant.

(Brower, 1992)

Selected as: F1 screen for mutations that fail to complement if³.

(Bloor and Brown, 1998)

Comments

Class 0 mutation.

(Bloor and Brown, 1998)

Very little protein made by this allele.

(Brower, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

if^B2

(Thuveson et al., 2019, Pérez-Moreno et al., 2017, Lin et al., 2013, Popodi et al., 2010-, Tikhmyanova et al., 2010, Jattani et al., 2009, Chanana et al., 2007, Ayyub and Paranjape, 2002)

Name Synonyms

inflatedB2

(Siekhaus et al., 2010)

Secondary FlyBase IDs

References (20)

Report Sections

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