FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Syt1AD3
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General Information
Symbol
Dmel\Syt1AD3
Species
D. melanogaster
Name
FlyBase ID
FBal0032846
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Reist et al., 1998)
Mutagen
ethyl methanesulfonate
(DiAntonio and Schwarz, 1994, Littleton et al., 1994, DiAntonio et al., 1993)
Progenitor genotype
Cytology
Description

Amino acid replacement: Y364N. Residue is in a highly conserved sequence within the second C2 domain.

(DiAntonio and Schwarz, 1994)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:2,785,667..2,785,667 [-]
Nucleotide change:

T2785667A

Reported nucleotide change:

T?A

Amino acid change:

Y364N | Syt1-PA; Y362N | Syt1-PB; Y362N | Syt1-PC; Y362N | Syt1-PE; Y363N | Syt1-PH; Y362N | Syt1-PI

Reported amino acid change:

Y364N

(DiAntonio and Schwarz, 1994)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Syt1AD3/Syt1T7 and Syt1AD3/Syt1T41 synapses have a rate constant that is not statistically different from wild-type controls.

      Heterozygous Syt1AD3 synapses have a rate constant that is not statistically different from wild-type controls.

      (Poskanzer et al., 2006)

      No asynchronous release following stimulation of the neuromuscular junction is seen in sytAD3/sytN13 mutants. The quantal content of synchronous release is reduced more than 10-fold, although the Ca2+ cooperativity is not altered compared to wild type. The total number of vesicles release during repetitive stimulation at the sytAD3/sytN13 neuromuscular junction is reduced approximately twofold compared to wild type, but the quantal content is reduced more than 10-fold. The frequency of spontaneous miniature synaptic currents at the embryonic sytAD3/sytN13 neuromuscular junction is not increased compared to wild type. The quantal content of nerve evoked release at the neuromuscular junction is reduced in heterozygotes compared to controls.

      (Yoshihara and Littleton, 2002)

      sytAD3/sytT11 mutants lack the on-off transients normally seen during electroretinogram recordings in the retina. The mutants do not show a depletion of synaptic vesicles at the photoreceptor synapses and synaptic vesicles can clearly be seen in contact with the presynaptic membrane under T-bars.

      (Littleton et al., 2001)

      A few sytAD3/Df(2L)DTD2 animals survive to adulthood. sytAD4/sytAD3 first larval instar nerve terminals contain fewer synaptic vesicles compared to wild-type and larger vesicles are more numerous in the mutant nerve terminals. The number of synaptic vesicles within 200nm of the presynaptic membrane is markedly reduced.

      (Reist et al., 1998)

      Transheterozygote combinations with other syt alleles are lethal and some adult survivors with sytT11, sytT41, syt66.4, sytD2, sytD37 and sytD45.

      (Littleton et al., 1994)

      Sluggish crawling and feeding behaviour. Larvae do not grow or moult as wild type larvae would, and die after a week (at 18oC).

      (DiAntonio et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      Syt1AD3 is partially rescued by Syt1elav.PD

      (DiAntonio and Schwarz, 1994)
      Comments

      Lethality can be rescued by sytelav.PD. Rescued flies that emerge are lethargic and show an electrophysiological deficit due to insufficient syt. A second copy of sytelav.PD improves viability and motor activity. ERGs of mutants carrying one copy of sytelav.PD lack both on- and off-transients suggesting a disruption in synaptic communication. In mutants the evoked neurotransmitter release is decreased and spontaneous release in increased (intracellular recordings made from muscle 6).

      (DiAntonio and Schwarz, 1994)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mutant phenotype cannot be rescued when homozygous but can when in trans to sytN6 by sytelav.PD.

      (Littleton et al., 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syt1AD3
      (Poskanzer et al., 2006)
      sytAD3
      sytAD4
      (Kang et al., 2014)
      Name Synonyms
      Secondary FlyBase IDs
        References (11)