FlyBase Allele Report: Dmel\Egfr::tor[t4021E.hs.sev]

General Information

Symbol

Dmel\Egfr::tor^{t4021E.hs.sev}

Species

D. melanogaster

Name

FlyBase ID

FBal0038834

Feature type

allele

Associated gene

Egfr::tor

Associated Insertion(s)

Carried in Construct

P{sE-torso⁴⁰²¹-DER}

Also Known As

sev-tor⁴⁰²¹Egfr

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Hime et al., 1997, Freeman, 1996, Lai et al., 1995, Reichman-Fried et al., 1994)

Progenitor genotype

Carried in construct

P{sE-torso⁴⁰²¹-DER}

Cytology

Description

Cytoplasmic domain of Egfr is fused to the extracellular domain from a constitutively active mutant of tor. Expression is influenced by sev enhancer and an Hsp70 promoter.

(Freeman, 1996)

Egfr intracellular kinase domain replaces the equivalent region from tor^13D, expression of the chimera is driven by an Hsp70 promoter and a single copy of the 1.2kb sev enhancer.

(Reichman-Fried et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

Egfr::tor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Bai et al., 2001, Casci et al., 1999)

Phenotype Manifest In

cone cell

(Freeman, 1996)

eye

(Bai et al., 2001, Casci et al., 1999, Dong et al., 1999)

mystery cell

(Freeman, 1996)

photoreceptor cell R7

(Dong et al., 1999)

primary pigment cell

(Freeman, 1996)

Detailed Description

Statement

Reference

Rough eye phenotype.

(Bai et al., 2001)

Rough eye phenotype. Extra R7 cells.

(Dong et al., 1999)

Homozygotes exhibit extra outer photoreceptor cells, the recruitment as neurons of the mystery cells. Ommatidia also exhibit extra cone and primary pigment cells, independent of the number of receptor cells. Activated Egfr is able to replace sev, sev¹⁴; P{sE-torso⁴⁰²¹-sev}/P{sE-torso⁴⁰²¹-sev} flies exhibit R7 cells in many ommatidia.

(Freeman, 1996)

Partial rescue of tracheal migration defect in btl homozygotes.

(Reichman-Fried et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Egfr::tor^{t4021E.hs.sev} has visible phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

Egfr::tor^{t4021E.hs.sev} has visible phenotype, enhanceable by sty[+]/sty^S73

(Casci et al., 1999)

Egfr::tor^{t4021E.hs.sev} has visible phenotype, enhanceable by sty[+]/sty^S88

(Casci et al., 1999)

Suppressed by

Statement

Reference

Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev has visible phenotype, suppressible by sty^UAS.N.Tag:HA, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev has visible phenotype, suppressible by sty^UAS.C.Tag:HA, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Suppressor of

Statement

Reference

Egfr::tor^{t4021E.hs.sev} is a suppressor of increased cell death phenotype of arm^S56F.GMR

(Freeman and Bienz, 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Egfr::tor^{t4021E.hs.sev} has eye phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

Egfr::tor^{t4021E.hs.sev} has eye phenotype, enhanceable by sty[+]/sty^S73

(Casci et al., 1999)

Egfr::tor^{t4021E.hs.sev} has eye phenotype, enhanceable by sty[+]/sty^S88

(Casci et al., 1999)

Suppressed by

Statement

Reference

Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev has eye phenotype, suppressible by sty^UAS.N.Tag:HA, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Egfr::tor^{t4021E.hs.sev}, Scer\GAL4^hs.2sev has eye phenotype, suppressible by sty^UAS.C.Tag:HA, Scer\GAL4^hs.2sev

(Casci et al., 1999)

Egfr::tor^{t4021E.hs.sev} has eye phenotype, suppressible by ebi^E90

(Dong et al., 1999)

Egfr::tor^{t4021E.hs.sev} has photoreceptor cell R7 phenotype, suppressible by ebi^E90

(Dong et al., 1999)

Egfr::tor^{t4021E.hs.sev} has eye phenotype, suppressible by Sos^34Ea-6

(Dong et al., 1999)

Egfr::tor^{t4021E.hs.sev} has photoreceptor cell R7 phenotype, suppressible by Sos^34Ea-6

(Dong et al., 1999)

Suppressor of

Statement

Reference

Egfr::tor^{t4021E.hs.sev} is a suppressor of eye phenotype of arm^S56F.GMR

(Freeman and Bienz, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Denticle belt fusions in the cuticles of rho^7M43; ru¹ double homozygous embryos are suppressed by Egfr::tor^{t4021E.hs.sev}; Scer\GAL4^prd.RG1.

(Urban et al., 2004)

The eye phenotype of flies expressing Egfr::tor^{t4021E.hs.sev} is dominantly enhanced by sty^S73 or sty^S88 and is suppressed by sty^{Scer\UAS.N.T:Ivir\HA1} or sty^{Scer\UAS.C.T:Ivir\HA1} expressed under the control of Scer\GAL4^hs.2sev.

(Casci et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Immunoprecipitation of protein lysates from flies expressing the activated Egfr::tor^{t4021E.hs.sev} receptor indicates that Shc is associated with the Egfr::tor^{t4021E.hs.sev} protein in vivo.

(Lai et al., 1995)

Lysates from non-heatshocked and heat shocked flies are generated and tested for protein binding to Cbl by SDS-gel electrophoresis.

(Meisner et al., 1997)

Immunoprecipitation of lysates from flies expressing Egfr::tor^{t4021E.hs.sev} indicates that Cbl protein is associated with activated Egfr protein.

(Hime et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Egfr::tor^{t4021E.hs.sev}

Egfr::tor^t4021E.hs

tor-DER

(Reichman-Fried et al., 1994)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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