FlyBase Allele Report: Dmel\Acsl[k10313]

General Information

Symbol

Dmel\Acsl^k10313

Species

D. melanogaster

Name

FlyBase ID

FBal0043454

Feature type

allele

Associated gene

Dmel\Acsl

Associated Insertion(s)

P{lacW}Acsl^k10313

Carried in Construct

Also Known As

l(2)k10313

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rottgen et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{lacW}Acsl^k10313

Cytology

Description

Inserted in the first intron of the RB and RD transcripts.

(Zhang et al., 2009)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,668,959..8,668,959 [+]

(Spradling et al., 1999, Rottgen et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with Acsl⁸)

(Zhang et al., 2009)

lethal | recessive

(Zhang et al., 2009, Spradling et al., 1999)

Phenotype Manifest In

lamina anlage glial cell (with Acsl⁸)

(Zhang et al., 2009)

lamina plexus (with Acsl⁸)

(Zhang et al., 2009)

larval brain | third instar larval stage (with Acsl⁸)

(Zhang et al., 2009)

medulla anlage (with Acsl⁸)

(Zhang et al., 2009)

Detailed Description

Statement

Reference

Neutral lipid accumulation is blocked in Acsl^k10313/Df(2R)H3E1 wing discs.

Total triacylglycerol content of Acsl^k10313/Df(2R)H3E1 pharates is significantly lower than wild type.

The normally defined pattern of axon projection in the optic lamina of the third instar larval brain is disrupted in the Acsl⁸/Acsl^k10313 mutant.

The following phenotypes are observed in the optic ganglia of Acsl⁸/Acsl^k10313 mutants: lamina neurons are significantly reduced in number, the normally fine alignment of glial cells disappears, the lamina plexus is in disarray, the number of glial cells in the medulla is greatly decreased, axon termination in the medulla is barely observed.

(Zhang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

NOT suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Enhancer of

Statement

Reference

Acsl^k10313 is an enhancer of visible phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

Suppressor of

Statement

Reference

l(2)44DEa[+]/Acsl^k10313 is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, suppressible by Hsap\ACSL4^{UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

NOT suppressed by

Statement

Reference

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Tub.PU/Hsap\ACSL4^{R570S.UAS.L.Tag:MYC}

(Zhang et al., 2009)

Acsl⁸/Acsl^k10313 has larval brain | third instar larval stage phenotype, non-suppressible by Hsap\ACSL4^{P375L.UAS.L.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Enhancer of

Statement

Reference

Acsl^k10313 is an enhancer of scutellar bristle phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

Suppressor of

Statement

Reference

l(2)44DEa[+]/Acsl^k10313 is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Mutation enhances the ectopic scutellar bristle phenotype seen from Scer\GAL4^455.2-mediated expression of l(1)sc^Scer\UAS.cHa.

(Rottgen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

(Zhang et al., 2009)

Complementation and Rescue Data

Fails to complement

Acsl⁰⁵⁸⁴⁷

(BDGP Project Members, 1994-1999)

Rescued by

Acsl⁸/Acsl^k10313 is rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^Tub.PU

(Zhang et al., 2009)

Not rescued by

Acsl⁸/Acsl^k10313 is not rescued by Acsl^{UAS.715.C.Tag:MYC}/Scer\GAL4^dpp.blk1

(Zhang et al., 2009)

Comments

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^tub restores well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

Expression of Hsap\ACSL4^{P375L.Scer\UAS.L.T:Hsap\MYC} or Hsap\ACSL4^{R570S.Scer\UAS.L.T:Hsap\MYC} under the control of Scer\GAL4^tub fails to restore well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

Expression of Acsl^{Scer\UAS.715.T:Hsap\MYC.C} under the control of Scer\GAL4^dpp.blk1 fails to restore well-ordered axon projection in the optic lamina of the third instar larval brain of Acsl⁸/Acsl^k10313 mutants.

(Zhang et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10433

y¹ w^67c23; P{lacW}Acsl^k10313/CyO

Kyoto

111095

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Acsl^k10313 /CyO y⁺

Notes on Origin