FlyBase Allele Report: Dmel\phyl[2245]

General Information

Symbol

Dmel\phyl²²⁴⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0044258

Feature type

allele

Associated gene

Dmel\phyl

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Karim et al., 1996, Chang et al., 1995)

Progenitor genotype

Cytology

Description

13bp frameshift deletion in the first exon of phyl, resulting in the generation of a premature stop codon 19 residues after the initial ATG.

(Chang et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:14,432,020..14,432,032 [-]

Comment:

A 13bp frameshift deletion in the first exon results in a premature stop 19 residues after the initial ATG.

(Chang et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | adult stage | somatic clone

(Yin and Xi, 2018)

decreased occurrence of cell division | adult stage | somatic clone

(Yin and Xi, 2018)

increased cell size | adult stage | somatic clone

(Yin and Xi, 2018)

lethal (with Df(2R)ED2354)

(Kahsai et al., 2016)

lethal | recessive

(Pi et al., 2001, Karim et al., 1996)

lethal - all die before end of larval stage | recessive

(Chang et al., 1995)

partially lethal (with phyl⁴)

(Pi et al., 2004)

partially lethal - majority live (with phyl⁴)

(Pi et al., 2001)

some die during larval stage | recessive

(Chang et al., 1995)

visible (with phyl⁴)

(Pi et al., 2001)

visible | somatic clone

(Pi et al., 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

phyl²²⁴⁵ homozygous clones in the adult midgut have a significantly decreased number of cells, as compared to controls; these clones virtually lack both mitotic cells and enteroendocrine cells, which are present in control clones, but include abnormally large and polyploid cells.

(Yin and Xi, 2018)

phyl²²⁴⁵ mutant somatic clones in pupal eye discs promote over-specification of primary pigment cell fates through the build-up of N internally.

(Nagaraj and Banerjee, 2009)

phyl⁴/phyl²²⁴⁵ flies have a greatly reduced number of bristles on the notum compared to wild type.

(Pi et al., 2004)

phyl²²⁴⁵/phyl⁴ adults lack some bristles and other bristles are abnormal. Homozygous clones have missing and abnormal bristles. More than 80% of the neurons and sheath cells of the es organs are missing in the dorsal-most region of the abdominal hemisegments in homozygous embryos, although only 12% of es organs are completely absent in this region. The dorsal-most region of the hemisegment usually contains 6 outer support cells (hair cells and socket cells) with one neuron and one sheath cell in homozygous embryos (instead of the wild-type 4 outer support cells, two neurons and two sheath cells), and occasionally 8 outer support cells are seen.

(Pi et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is an enhancer of visible phenotype of sina³/sina²

(Pi et al., 2001)

NOT Enhancer of

Statement

Reference

phyl²²⁴⁵ is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

phyl²²⁴⁵ is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is a suppressor of decreased occurrence of cell division phenotype of CycE^JP

(Brumby et al., 2004)

phyl²²⁴⁵ is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

NOT Suppressor of

Statement

Reference

phyl²²⁴⁵ is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

phyl²²⁴⁵ is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Other

Statement

Reference

Ras85D^V12.sev, phyl²²⁴⁵, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

phyl²²⁴⁵ has primary pigment cell | increased number phenotype, suppressible by mib¹

(Nagaraj and Banerjee, 2009)

phyl²²⁴⁵ has photoreceptor cell R1 phenotype, suppressible by mib¹

(Nagaraj and Banerjee, 2009)

phyl²²⁴⁵ has photoreceptor cell R6 phenotype, suppressible by mib¹

(Nagaraj and Banerjee, 2009)

Enhancer of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is an enhancer of adult abdominal segment 3 & microchaeta phenotype of sina³/sina²

(Pi et al., 2001)

phyl[+]/phyl²²⁴⁵ is an enhancer of adult abdominal segment 4 & microchaeta phenotype of sina³/sina²

(Pi et al., 2001)

phyl[+]/phyl²²⁴⁵ is an enhancer of mesothoracic tergum & chaeta phenotype of sina³/sina²

(Pi et al., 2001)

phyl[+]/phyl²²⁴⁵ is an enhancer of mesothoracic tergum & microchaeta phenotype of sina³/sina²

(Pi et al., 2001)

phyl[+]/phyl²²⁴⁵ is an enhancer of mesothoracic tergum & tormogen cell | supernumerary phenotype of sina³/sina²

(Pi et al., 2001)

phyl[+]/phyl²²⁴⁵ is an enhancer of mesothoracic tergum & trichogen cell | supernumerary phenotype of sina³/sina²

(Pi et al., 2001)

NOT Enhancer of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is a non-enhancer of phenotype of L^rev6-3

(Son and Choi, 2020)

phyl²²⁴⁵ is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

phyl²²⁴⁵ is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is a suppressor of eye disc & S phase phenotype of CycE^JP

(Brumby et al., 2004)

phyl[+]/phyl²²⁴⁵ is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2004)

phyl²²⁴⁵ is a suppressor of phenotype of Ras85D^V12.sev

(Karim et al., 1996, Chang et al., 1995)

NOT Suppressor of

Statement

Reference

phyl[+]/phyl²²⁴⁵ is a non-suppressor of phenotype of L^rev6-3

(Son and Choi, 2020)

phyl²²⁴⁵ is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

phyl²²⁴⁵ is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The over-specification of primary pigment cell fates found in phyl²²⁴⁵ mutant somatic clones is suppressed in phyl²²⁴⁵ mib¹ double mutants. These double mutants also exhibit significant rescue in the specification of B-H1-expressing R1 and R6 cell types.

(Nagaraj and Banerjee, 2009)

Dominantly suppresses the S phase defects seen in the CycE^JP eye disc.

(Brumby et al., 2004)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by phyl²²⁴⁵.

(Maixner et al., 1998)

Suppresses the rough eye phenotype of Ras85D^V12.sev. Slightly suppresses the phenotype of Ras85D^N17.sev. Suppresses the phenotype of phl::tor^13D.hs.sev. Suppresses the phenotype of Ras85D::Src64B^{V12.ΔCAAX.sev}. Has little or no effect on the lethality of phl¹². Has little or no effect on the phl¹² eye phenotype. Has little or no effect on the Dsor1^XS520 eye phenotype. Has little or no effect on the aop^S2382 eye phenotype.

(Karim et al., 1996)

Dominantly suppresses the rough eye and supernumerary R7 phenotype of Ras85D^V12.sev : 43% of the ommatidia in the center of the eye are wild type. Dominantly suppresses the rough eye and supernumerary R7 phenotype of phl::tor^13D.hs.sev. Dead larvae have normally patterned cuticle.

(Chang et al., 1995)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

phyl^28S2

(Brumby et al., 2004)

phyl^38S4

(Brumby et al., 2004)

phyl^39S2

(Brumby et al., 2004)

Partially rescued by

phyl⁴/phyl²²⁴⁵ is partially rescued by phyl^3.4.ORF

(Pi et al., 2004)

phyl⁴/phyl²²⁴⁵ is partially rescued by phyl^3.4ΔES.ORF

(Pi et al., 2004)

phyl⁴/phyl²²⁴⁵ is partially rescued by phyl^3.4ΔE.ORF

(Pi et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5686

phyl²²⁴⁵/CyO, P{sevRas1.V12}FK1

Kyoto

108363

phyl²²⁴⁵/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

phyl²²⁴⁵

(Son and Choi, 2020, Duan et al., 2018, Yin and Xi, 2018, Dollar et al., 2016, Hwang and Rulifson, 2011, Nagaraj and Banerjee, 2009, Christensen and Cook, 2007.10.29, Settle et al., 2003)

phyl^XS-2245

(Maixner et al., 1998)

Name Synonyms

Secondary FlyBase IDs

FBal0050265

References (17)

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