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General Information
Symbol
Dmel\sina2
Species
D. melanogaster
Name
FlyBase ID
FBal0015666
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G16857047A

Reported nucleotide change:

G1532A

Amino acid change:

W210term | sina-PA; W210term | sina-PB; W210term | sina-PC

Reported amino acid change:

W210term

Comment:

TGG to TGA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation results in a truncated protein that lacks the C-terminal 105 amino acids of the wild-type protein.

Nucleotide substitution: G1532A.

Amino acid replacement: W210term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

adult abdominal segment 3 & chaeta (with sina3)

adult abdominal segment 3 & microchaeta (with sina3)

adult abdominal segment 3 & tormogen cell | supernumerary (with sina3)

adult abdominal segment 3 & trichogen cell | supernumerary (with sina3)

adult abdominal segment 4 & chaeta (with sina3)

adult abdominal segment 4 & microchaeta (with sina3)

adult abdominal segment 4 & tormogen cell | supernumerary (with sina3)

adult abdominal segment 4 & trichogen cell | supernumerary (with sina3)

mesothoracic tergum & chaeta (with sina3)

mesothoracic tergum & microchaeta (with sina3)

mesothoracic tergum & tormogen cell | supernumerary (with sina3)

mesothoracic tergum & trichogen cell | supernumerary (with sina3)

Detailed Description
Statement
Reference

sina2 homozygous clones in the adult midgut bear virtually no enteroendocrine cells, unlike the expected 6-8% in control clones, but exhibit a similar number of cells as compared to controls.

The number of pale-type R8 cells is dramatically reduced in the eyes of sina2/sina3 animals.

A small percentage of sina2/sina3 mutant third instar larval eye discs show a reduction in the number of R1 and R6 photoreceptor cells. Very few R7 cells are recruited to the developing ommatidia in these mutants.

sina2/sina3 lack R7 photoreceptors.

Mutants show a double- bristle phenotype.

41% of the notal microchaetae and 62% of the microchaetae on abdominal segments 3 and 4 are missing in sina2/sina3 flies. 17.2% of bristles on the notum are duplicated, having 2 hairs emerging from 2 sockets or a fused socket. 44.5% of bristles on abdominal segments 3 and 4 are duplicated (2 hairs/2 sockets) and 20.9% show other defects (including 1 hair surrounded by 2-3 sockets, and 2 socket or 4 socket clusters without shafts). Embryonic development, including development of the es organs, is normal in sina2/sina3 animals.

sina2/sina3 mutants show missing R7 cells in 90% of the ommatidia. The external morphology of the eye shows a slight roughness.

Proportion of ommatidia with an R7 cell is less than 5%.

Amorph based on the phenotypes of hemizygous <up>sina1/Df(3L)st-g18</up> versus that of homozygous flies.

This allele probably represents complete loss of function of the sina gene. In the ommatidia the R7 photoreceptor cell is always missing, the R7 precursor adopting a cone cell fate, and sometimes one or two cells of the R1-R6 class are missing as well. The normal trapezoidal arrangement of the R1-R6 cells is disorganised, and the R8 cell is often found in uncharacteristic positions. Other adult sensory organs are also affected: sensory bristles distributed over the body surface are frequently missing, with neither the cuticular bristle sheath nor the socket being present, affected sensory organs including the microchaetae and macrochaetae of the head, notum, abdomen and wing margin, and the stout and slender bristles of the anterior wing margin. The phenotype is most severe with bristles located on the abdomen, where often only a minor fraction of the normal number of bristles are present. Also, many of the remaining bristles have two or three sheaths emerging from a single socket. There is also a 10-fold reduction in adult lifespan, and adult behaviour is generally lethargic and uncoordinated. Neither males nor females are fertile, although they produce morphologically normal sperm and eggs, respectively.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

sina3/sina2 has visible phenotype, enhanceable by msi1/msi1

sina3/sina2 has visible phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has visible phenotype, enhanceable by phyl[+]/phyl2245

Suppressed by
Statement
Reference
Enhancer of
Statement
Reference

sina3/sina2 is an enhancer of visible | recessive phenotype of msi1

sina3/sina2 is an enhancer of visible phenotype of msi1

Phenotype Manifest In
Enhanced by
Statement
Reference

sina3/sina2 has adult abdominal segment 3 & chaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has mesothoracic tergum & chaeta phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has mesothoracic tergum & tormogen cell | supernumerary phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has mesothoracic tergum & trichogen cell | supernumerary phenotype, enhanceable by phyl[+]/phyl2245

sina3/sina2 has adult abdominal segment 3 & microchaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has adult abdominal segment 4 & chaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has adult abdominal segment 4 & microchaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has mesothoracic tergum & chaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has mesothoracic tergum & microchaeta phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has mesothoracic tergum & tormogen cell | supernumerary phenotype, enhanceable by phyl2/phyl[+]

sina3/sina2 has mesothoracic tergum & trichogen cell | supernumerary phenotype, enhanceable by phyl2/phyl[+]

Suppressed by
Statement
Reference

sina3/sina2 has ommatidium phenotype, suppressible by edslH8/ed1X5

sina3/sina2 has mesothoracic tergum & chaeta phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has mesothoracic tergum & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has mesothoracic tergum & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 3 & chaeta phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 3 & microchaeta phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 3 & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 3 & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 4 & chaeta phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 4 & microchaeta phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 4 & tormogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

sina3/sina2 has adult abdominal segment 4 & trichogen cell | supernumerary phenotype, suppressible by ttk[+]/ttkosn

msi1, sina3/sina2 has ommatidium phenotype, suppressible by ttkosn

msi1, sina3/sina2 has rhabdomere phenotype, suppressible by ttkosn

msi1, sina3/sina2 has cone cell phenotype, suppressible by ttkosn

sina2 has phenotype, suppressible | partially by ttkosn

NOT suppressed by
Statement
Reference

sina2 has photoreceptor cell R7 phenotype, non-suppressible by sl1

sina2 has photoreceptor cell R7 phenotype, non-suppressible by sl2

Enhancer of
Statement
Reference

sina3/sina2 is an enhancer of external sensory organ phenotype of msi1

sina3/sina2 is an enhancer of ommatidium phenotype of msi1

Suppressor of
Statement
Reference

sina[+]/sina2 is a suppressor | partially of eye phenotype of CycEJP

sina2 is a suppressor of photoreceptor cell R7 phenotype of sty254

sina2 is a suppressor of phenotype of phylhs.sev

sina3/sina2 is a suppressor of eye phenotype of phylGMR.PC

Other
Additional Comments
Genetic Interactions
Statement
Reference

The absence of enteroendocrine cells in adult midgut sina2 clones is suppressed by the clonal expression of ttkGD4414 under the control of Scer\GAL4Act.PU, even leading to their severe increase in number as compared to control clones.

In sina2,msi1/sina3,msi1 mutant third instar larval eye discs significantly reduced numbers of R1 and R6 photoreceptor cells are observed. Very few R7 cells are recruited to the developing ommatidia in these mutants, decreased in number compared to sina2/sina3 mutant animals.

Expression of ttkGD4414 under the control of Scer\GAL4lz-gal4 in sina2,msi1/sina3,msi1 mutant third instar larval eye discs, significantly rescues the number of R1 and R6 cells, although recruitment of these cells appears delayed. Ectopic R7 cells are observed in most developing ommatidia.

Double mutants of sina2/sina3 with msi1 show a synergistic, not additive, eye phenotype. Ommatidial arrays are disturbed, eyes consequently roughened and ommatidia do not contain more than five rhabdomeres. Cone cell array is disturbed. 30% of the ommatidia of sina2 msi1 ttkosn/sina3 msi1 show the normal number and arrangement of photoreceptor cells. The cone cell defects of sina2 msi1/sina3 msi1 are also suppressed by ttkosn.

No R7 cells form in sty254, sina2 double mutant ommatidia.

Double mutants of sl1 or sl2 with sina2 show no increase in proportion of R7 cells over that seen for sina2 alone.

The phylGMR.PC eye phenotype is completely suppressed in a sina2/sina3 background; the eyes are normal, except for the lack of R7 photoreceptor cells due to the absence of sina function. sina2/sina3 flies carrying both phylGMR.PC and ttk88.GMR.T:Hsap\MYC have very disrupted eyes, similar to those of flies carrying ttk88.GMR.T:Hsap\MYC alone.

Ommatidia of double mutants with Gap1B2 do not have multiple R7 cells.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Ecol\CAT activity in sina2 mutant flies transformed with either Ecol\CATRh3.PF or Ecol\CATRh4.PF is reduced by 90% or eliminated respectively, confirming that R7 was absent from each ommatidium in sina2 mutants. Ecol\lacZ82 expression is abolished in the R7 precursor cell in sina2 mutants.

No interaction with P{sev-svp1} or P{sev-svp2} exists.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (21)