FlyBase Allele Report: Dmel\trpl[302]

General Information

Symbol

Dmel\trpl³⁰²

Species

D. melanogaster

Name

FlyBase ID

FBal0050670

Feature type

allele

Associated gene

Dmel\trpl

Associated Insertion(s)

Carried in Construct

Also Known As

trpl^p302

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Niemeyer et al., 1996)

loss of function allele

(Reuss et al., 1997)

Mutagen

ethyl methanesulfonate

(Niemeyer et al., 1996)

Progenitor genotype

Cytology

Description

Mutation is predicted to truncate the protein before the first transmembrane segment.

(Niemeyer et al., 1996)

Amino acid replacement: ?302term.

(Niemeyer et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:9,761,962..9,761,962 [+]

Nucleotide change:

C9761962T

Amino acid change:

Q302term | trpl-PA; Q302term | trpl-PB; Q302term | trpl-PD

Comment:

site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change

(Niemeyer et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

L-type sensillum L2, with Scer\GAL4^Gr5a.PUn, trpl^UAS.cZa

(Zhang et al., 2013)

Malpighian tubule

(Macpherson et al., 2005)

photoreceptor

(Reuss et al., 1997)

photoreceptor cell & axon

(Niemeyer et al., 1996)

photoreceptor neuron

(Haab et al., 2000, Leung et al., 2000, Chyb et al., 1999, Li et al., 1999)

short labellar taste bristle S-a

(Zhang et al., 2013)

Detailed Description

Statement

Reference

trpl³⁰² mutant adults show normal preference for less solid sucrose-agarose food than a harder one (higher agarose conc., same sucrose content) one in a two-way food choice assay.

(Zhang et al., 2016)

Mutant flies show a deficit in gustatory aversion to 6mM camphor in a two way-choice test. Camphor-induced action potentials in S6 taste sensilla are greatly reduced in the mutant flies compared to wild type.

Mutant flies show normal olfactory avoidance of 6mM camphor in a direct airborne repellent test.

Mis-expression of trpl^Scer\UAS.cZa in L2 sensilla under the control of Scer\GAL4^Gr5a.PUn in a trpl³⁰² background results in these sensilla showing a physiological responses to camphor, but not to other bitter tastants such as quinine and strychnine.

(Zhang et al., 2013)

trpl³⁰² homozygotes show decreased avoidance to CO2, as compared to heterozygous and wild-type controls; in agreement, mutants show decreased electrophysiological responses of the basiconica rich region of the 3rd antennal segment to CO2 exposure, as compared to controls. These homozygotes do not exhibit changes in the number of ab1C neurons.

(Badsha et al., 2012)

Compound action potentials can be evoked by sound in the antennal nerve of mutant flies, but the sound particle velocities required to elicit the response is increased compared to wild type. Nonlinear mechanical amplification is significantly reduced compared to wild type.

(Senthilan et al., 2012)

trpl³⁰² mutants show a sustained response to prolonged light.

(Richter et al., 2011)

Mutant flies exhibit the wild type avoidance of aristolochic acid in food choice assays.

(Kim et al., 2010)

Mutant adults show normal avoidance of 1% citronellal in a direct airborne repellent test (DART) assay.

(Kwon et al., 2010)

The normal preference of third instar larvae for 17.5[o]C over 14[o]C in a two-way choice test is eliminated in trpl³⁰² and trpl³⁰²/trpl^MB03075 larvae.

(Kwon et al., 2010)

trpl³⁰² mutant class IV dendritic arborization neurons do not exhibit a statistically significant difference in firing in response to light.

(Xiang et al., 2010)

trpl³⁰² mutants retain a preference for 18[o]C (i.e. are thermotactic).

(Kwon et al., 2008)

capa stimulated fluid transport rates are significantly reduced in Malpighian tubules from trpl³⁰² animals compared to control tubules, while the basal rate of secretion is unaffected.

(Macpherson et al., 2005)

Mutant flies show an electroretinogram response that is similar to wild-type in response to a 10 second pulse of light.

(Wang et al., 2005)

The reduction in electroretinogram (ERG) amplitude seen in flies exposed to constant light is not suppressed by trpl³⁰².

(Lee and Montell, 2004)

Wild-type flies kept in the dark are significantly more sensitive to background light than wild-type flies kept in the light or trpl³⁰² flies kept in light or darkness.

(Baehner et al., 2002)

Mutant phenotype can be detected using ERG and intracellular recording techniques after prolonged stimuli. The receptor potential has a smaller sustained component than wild type, with altered time course of decay, though peak responses are similar. The response is accompanied by oscillations during stimulus and hyperpolarization after stimulus termination. Mutants also show inability to adapt to dim background illuminations. Refractory period and response latency are wild type.

(Leung et al., 2000)

When IBMX is applied to trpl³⁰² mutant flies the macroscopic response is slowed in a way that is no different to that of wild type flies exposed to IBMX.

(Chyb et al., 1999)

E_rev in mutant photoreceptors shows a small but statistically highly significant positive shift compared to wild type. Light sensitive conductance is even more permeable to Na⁺ than wild type. Permeability to Li⁺ is roughly the same in wild type, trpl³⁰² and trp² mutants. In general the permeability of mutant photoreceptors to Ca²⁺, Na⁺, Li⁺, Ba²⁺, Mg²⁺ and Mn²⁺ is higher than that for wild type. The I-V relationship of the light sensitive conductance is similar to that of wild type in always showing a pronounced dual rectification. Shows no biphasic reversal potential behaviour with lowered external calcium. Estimated single channel conductance is 4pS. La³⁺ completely blocks the light response. Light sensitive conductance is facilitated by Ca²⁺.

(Reuss et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

trpl³⁰² has abnormal visual behavior phenotype, enhanceable by trp²

(Li et al., 1999)

trpl³⁰² has abnormal visual behavior phenotype, enhanceable by trp⁹

(Li et al., 1999)

NOT Enhanced by

Statement

Reference

trpl³⁰² has abnormal smell perception | dominant phenotype, non-enhanceable by trp⁹/trp⁹

(Badsha et al., 2012)

trpl³⁰² has abnormal neurophysiology | recessive phenotype, non-enhanceable by trp⁹/trp⁹

(Badsha et al., 2012)

Enhancer of

Statement

Reference

trpl³⁰² is an enhancer of abnormal neurophysiology phenotype of Xport-A¹

(Rosenbaum et al., 2011)

NOT Enhancer of

Statement

Reference

trpl³⁰²/trpl³⁰² is a non-enhancer of abnormal smell perception | dominant phenotype of trp⁹

(Badsha et al., 2012)

trpl³⁰²/trpl³⁰² is a non-enhancer of abnormal neurophysiology | recessive phenotype of trp⁹

(Badsha et al., 2012)

Other

Statement

Reference

trp⁹, trpl³⁰² has abnormal neurophysiology | recessive phenotype

(Badsha et al., 2012)

trp⁹, trpl³⁰² has abnormal neurophysiology phenotype

(Krause et al., 2008, Huang et al., 2004)

trp⁹, trp^D621G, trpl³⁰² has abnormal neurophysiology | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621E, trpl³⁰² has abnormal neurophysiology | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621N, trpl³⁰² has abnormal neurophysiology | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621N, trpl³⁰² has abnormal neuroanatomy | adult stage | progressive phenotype

(Liu et al., 2007)

inaD^P215, trpl³⁰² has abnormal visual behavior phenotype

(Leung et al., 2000)

trp², trpl³⁰² has abnormal visual behavior phenotype

(Leung et al., 2000, Reuss et al., 1997)

trp⁹, trpl³⁰² has abnormal circadian rhythm | recessive phenotype

(Yang et al., 1998)

trp¹, trpl³⁰² has abnormal visual behavior phenotype

(Reuss et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

trpl³⁰² has photoreceptor neuron phenotype, enhanceable by trp²

(Li et al., 1999)

trpl³⁰² has photoreceptor neuron phenotype, enhanceable by trp⁹

(Li et al., 1999)

Suppressor of

Statement

Reference

trp¹/trpl³⁰² is a suppressor of ommatidium & microvillus phenotype of rdgA¹

(Raghu et al., 2000)

trp²/trpl³⁰² is a suppressor of ommatidium & microvillus phenotype of rdgA¹

(Raghu et al., 2000)

trpl³⁰²/trp⁹ is a suppressor of ommatidium & microvillus phenotype of rdgA¹

(Raghu et al., 2000)

trp¹/trpl³⁰² is a suppressor of ommatidium phenotype of rdgA¹

(Raghu et al., 2000)

trp²/trpl³⁰² is a suppressor of ommatidium phenotype of rdgA¹

(Raghu et al., 2000)

trpl³⁰²/trp⁹ is a suppressor of ommatidium phenotype of rdgA¹

(Raghu et al., 2000)

NOT Suppressor of

Statement

Reference

trpl³⁰² is a non-suppressor of retina | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

trpl³⁰² is a non-suppressor of photoreceptor neuron | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

trpl³⁰² is a non-suppressor of rhabdomere of eye photoreceptor cell | adult stage phenotype of Culd^e01982

(Xu and Wang, 2016)

trpl³⁰² is a non-suppressor of phenotype of rdgA¹

(Raghu et al., 2000)

Other

Statement

Reference

trp⁹, trpl³⁰² has ommatidium phenotype

(Krause et al., 2008)

trp⁹, trp^D621G, trpl³⁰² has photoreceptor | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621E, trpl³⁰² has photoreceptor | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621N, trpl³⁰² has photoreceptor | adult stage phenotype

(Liu et al., 2007)

trp⁹, trp^D621N, trpl³⁰² has rhabdomere | adult stage | progressive phenotype

(Liu et al., 2007)

trp⁹, trpl³⁰² has retina phenotype

(Huang et al., 2004)

trp⁹, trpl³⁰² has photoreceptor neuron phenotype

(Agam et al., 2000, Haab et al., 2000)

inaD^P215, trpl³⁰² has photoreceptor neuron phenotype

(Leung et al., 2000)

trp², trpl³⁰² has photoreceptor neuron phenotype

(Leung et al., 2000)

trp¹, trpl³⁰² has photoreceptor neuron phenotype

(Chyb et al., 1999)

trp¹, trpl³⁰² has photoreceptor phenotype

(Reuss et al., 1997)

trp², trpl³⁰² has photoreceptor phenotype

(Reuss et al., 1997)

trp², trpl³⁰² has photoreceptor cell & axon phenotype

(Niemeyer et al., 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The progressive light-dependent retinal degeneration (loss of photoreceptor cells and rhabdomeres) characteristic for Culd^e01982 mutant adults is not suppressed by combination with trpl³⁰².

(Xu and Wang, 2016)

Electroretinograms of trpl³⁰², trp⁹ double homozygotes show a complete lack of response to light stimuli, as compared to either single mutant.

(Badsha et al., 2012)

The trpl³⁰²; trp⁹ double mutant has no functional trp and trpl channels and shows no response to light.

(Richter et al., 2011)

Dissociated Xport¹, trpl³⁰² double mutant ommatidia show a residual response to brief light flashes; sensitivity is reduced ~500-fold with respect to wild type and 25-fold with respect to Xport¹.

(Rosenbaum et al., 2011)

Illumination of dissociated, patch-clamped ommatidia from trp⁹;trpl³⁰² double mutants show activation of the whole phototransduction cascade, do not show any light-induced current or Ca[2+] influx, but do show a normal light-dependent modulation of the delayed rectifier current mediated by Shab channels. Increasing the light level by a factor of ten results in an upregulation of approximately 30% and recovery to baseline within 30 minutes, similar to wildtype controls.

(Krause et al., 2008)

trp⁹; trpl³⁰² individual photoreceptors form the correct number of synapses per

presynaptic terminal independently of cartridge composition. The photoreceptor terminals of these mutants show an increase in glial invaginations.

(Hiesinger et al., 2006)

capa stimulated fluid transport rates are significantly reduced in Malpighian tubules from trpl³⁰² trp⁹ animals compared to control tubules, while the basal rate of secretion is unaffected.

(Macpherson et al., 2005)

trpl³⁰² ; trp¹⁴ flies show a electroretinogram response in response to a 10 second pulse of light that is similar to that seen in trp¹⁴ single mutants.

trpl³⁰² ; trp^L612F.ninaE trp⁹ flies have a wild-type electroretinogram response.

trpl³⁰² ; trp^ninaE.PW trp⁹ flies have a wild-type electroretinogram response.

trpl³⁰² ; trp^R671Q.ninaE trp⁹ flies have a transient electroretinogram response to a 10 second pulse of light.

(Wang et al., 2005)

trp⁹ ; trpl³⁰² double mutants show a reduced depolarisation response to anoxia in the photoreceptors compared to wild-type flies; the amplitude of anoxia-induced depolarisation in the double mutants is half that of wild type and the response kinetics are much slower than normal.

(Huang et al., 2004)

In trp⁹,trpl³⁰² double mutants the second, large phase depolarization response to anoxia (caused by N₂ and measured in the eye by extracellular voltage change recordings), fails, indicating that the small but not the large phase of the response resulted from accumulation of K⁺ in the extracellular space. In trp⁹, trpl³⁰² double mutants the Ca²⁺ response to anoxia is virtually abolished.

(Agam et al., 2000)

Excised patches from rhabdomeral membranes show no sustained spontaneous activity nor high frequency burst-like behavior in trp⁹,trpl³⁰² double mutants.

(Haab et al., 2000)

Rhabdomeres of trpl³⁰²,inaD¹ double mutants are intact in 1 day old flies. ERG light responses of trpl³⁰²,inaD¹ and trpl³⁰²,trp² are small and transient, but the response amplitude of trpl³⁰²,inaD¹ is significantly smaller than that of trpl³⁰²,trp². Similarly, refractory period and response latency defects are more severe for trpl³⁰²,inaD¹ than for trpl³⁰²,trp².

(Leung et al., 2000)

In trp¹, trpl³⁰² double mutants light sensitive channels are not activated by the application of linolenic acid, whereas they are activated in single mutant channels.

(Chyb et al., 1999)

Double mutant analysis comparing the receptor potentials of trpl³⁰² with inaF^P106x; trpl³⁰² or trp²; trpl³⁰² or trp⁹; trpl³⁰² showed that the effects of inaF^P106x are as severe as those of trp² and are specific to the trp channel.

(Li et al., 1999)

Behavioural rhythms of trp⁹ trpl³⁰² double mutants show reduced resetting in response to a pulse of light.

(Yang et al., 1998)

trpl³⁰²; trp² double mutants are almost blind as judged by response to saturating ultraviolet flashes and ERG recordings. trpl³⁰²; trp¹ double mutants are blind.

(Reuss et al., 1997)

Whole-cell patch-clamp recordings of photoreceptors display sensitivity and response kinetics indistinguishable from wild type. trpl³⁰²; trp² mutants display a dramatic loss of responsiveness, the residual current is due to a small amount of functional trp channel. trpl³⁰²; trp² mutants exhibit a dramatic bump phenotype.

(Niemeyer et al., 1996)

Xenogenetic Interactions

Statement

Reference

The presence of trp::trpl^{ninaE.trp1-675.trpl681-1124.T:Avic\GFP-EGFP} in trpl³⁰²; trp⁹ double mutants results in a response to light that declines towards baseline during prolonged intense light.

The presence of trp::trpl^{ninaE.trpl1-336.T:Avic\GFP-EGFP} in trpl³⁰²; trp⁹ double mutants results in a response to light that declines towards baseline during prolonged intense light.

The presence of trp::trpl^{ninaE.trpl1-336.trp328-675.trpl681-1124.T:Avic\GFP-EGFP} in trpl³⁰²; trp⁹ double mutants results in a response to light that declines towards baseline during prolonged intense light.

The presence of trp::trpl^{ninaE.trp1-328.trpl336-681.trp675-1275.T:Avic\GFP-EGFP} in trpl³⁰²; trp⁹ double mutants results in a response to light that declines towards baseline during prolonged intense light.

(Richter et al., 2011)

Complementation and Rescue Data

Rescued by

trpl³⁰² is rescued by trpl^UAS.cZa/Scer\GAL4^Gr66a.PD

(Zhang et al., 2013)

trpl³⁰² is rescued by Scer\GAL4^trpl.PZ/trpl^UAS.cZa

(Zhang et al., 2013)

trpl³⁰² is rescued by Scer\GAL4^Gr21a.PC/trpl^UAS.cNa

(Badsha et al., 2012)

trpl³⁰² is rescued by trpl^hs.PN

(Macpherson et al., 2005)

trpl³⁰² is rescued by trpl^ninaE.PW

(Wang et al., 2005)

trpl³⁰² is rescued by trpl^H678R.ninaE

(Wang et al., 2005)

trpl³⁰² is rescued by trpl^hs.PN

(Niemeyer et al., 1996)

Comments

Expression of trpl^Scer\UAS.cZa under the control of either Scer\GAL4^Gr66a.PD or Scer\GAL4^trpl.PZ restores normal camphor avoidance and camphor-induced action potentials in S6 sensilla in trpl³⁰² flies.

(Zhang et al., 2013)

Reintroduction of trpl^hs.PN to trpl³⁰²; trp² mutants rescues the defect and restores visual physiology. Photoreceptor cells produce quantum bumps (the unitary event of a single photon) that are indistinguishable from wild type.

(Niemeyer et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31433

cn¹ trpl³⁰² bw¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

trpL³⁰²

(Wong et al., 2014)

trpl³⁰²

(Nath et al., 2025, Delgado et al., 2024, Mi et al., 2023, Rhodes-Mordov et al., 2023, Tian et al., 2022, Zhang et al., 2022, Chen and Montell, 2020, Ma and Freeman, 2020, Schopf et al., 2019, Asteriti et al., 2017, Katz et al., 2017, Yasin et al., 2017, Xu and Wang, 2016, Zhang et al., 2016, Hardie et al., 2015, Delgado et al., 2014, Cerny et al., 2013, Katz et al., 2013, Zhang et al., 2013, Badsha et al., 2012, Senthilan et al., 2012, Richter et al., 2011, Rosenbaum et al., 2011, Vasiliauskas et al., 2011, Chen et al., 2010, Elsaesser et al., 2010, Kim et al., 2010, Kwon et al., 2010, Kwon et al., 2010, Satoh et al., 2010, Xiang et al., 2010, Krause et al., 2008, Rosenzweig et al., 2008, Liu et al., 2007, Hiesinger et al., 2006, Meyer et al., 2006, Rosenbaum et al., 2006, Wang et al., 2005, Huang et al., 2004)

trpl^P302

(Kwon et al., 2008)

trpl^p302

(Haab et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (60)

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