FlyBase Allele Report: Dmel\br[BRcore.Q1.Z1.hs]

General Information

Symbol

Dmel\br^{BRcore.Q1.Z1.hs}

Species

D. melanogaster

Name

FlyBase ID

FBal0057741

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

P{BRcore-Q¹-Z1}

Key Links

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Crossgrove et al., 1996)

wild_type

(Crossgrove et al., 1996)

Mutagen

in vitro construct

(Bayer et al., 1997, Crossgrove et al., 1996)

Progenitor genotype

Carried in construct

P{BRcore-Q¹-Z1}

Cytology

Description

br cDNA dm527 is expressed from a heat inducible Hsp70 promoter.

(Crossgrove et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Over-expression of four copies of the br Z1 isoform (using a line containing both br^{BRcore.TNT.Q1.Z1.hs} and br^{BRcore.Q1.Z1.hs}) using heat shock at 5 hours after ecdysis to the second instar causes lethality at approximately 42-68 hours after the heat shock with no detectable third instar mouthhooks.

(Zhou et al., 2004)

Animals carrying two copies of br^{BRcore.TNT.Q1.Z1.hs} and two copies of br^{BRcore.Q1.Z1.hs} that have been heat shocked between 45 and 52 hours after puparium formation (APF) form pharate adults with a transparent cuticle on many areas of the head, thorax and abdomen. The microchaetae and macrochaetae are present, and are sometimes slightly tanned. Dorsolateral areas of the abdomen sometimes show the beginning of adult melanisation that extends into the anterior region of the tergite where melanisation is not normally found. Animals carrying two copies of br^{BRcore.TNT.Q1.Z1.hs} and two copies of br^{BRcore.Q1.Z1.hs} that have been heat shocked between 30-35 hours APF show truncation of bristles on the head and thorax. Animals carrying two copies of br^{BRcore.TNT.Q1.Z1.hs} and two copies of br^{BRcore.Q1.Z1.hs} that have been heat shocked between 35-39 hours APF show truncation of bristles on the abdomen. Animals carrying two copies of br^{BRcore.TNT.Q1.Z1.hs} and two copies of br^{BRcore.Q1.Z1.hs} that have been heat shocked at 35 or 39 hours APF lack abdominal trichomes.

(Zhou and Riddiford, 2002)

A 37^oC heat shock causing expression of br^{BRcore.Q1.Z1.hs} during the third larval instar or early prepupal stage results in lethality within a few hours of the heat shock. Heat shock induced expression of br^{BRcore.Q1.Z1.hs} during late prepupal or pupal stages has no effect.

(Bayer et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions