FlyBase Allele Report: Dmel\wg[UAS.cKa]

General Information

Symbol

Dmel\wg^UAS.cKa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Krause

FlyBase ID

FBal0065586

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.K}

Also Known As

UAS-wg, P^UAS-wg

Key Links

Transgenic product class

wild_type

(Hacker et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hacker et al., 1997)

Mutagen

in vitro construct

(Hacker et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-wg.K}

Cytology

Description

Wild-type wg is expressed under the control of UAS regulatory sequences.

(Hacker et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Chang et al., 2008)

visible, with Scer\GAL4^ey.PH

(Ekas et al., 2006)

visible, with Scer\GAL4^GMR.PF

(Penton et al., 2002)

visible | adult stage, with Scer\GAL4^GMR.PF

(Chang et al., 2008)

Phenotype Manifest In

abdominal sternite 6 | male limited, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

abdominal sternite 7 | ectopic | male limited, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

adult abdominal segment 7 | ectopic | male limited, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

denticle, with Scer\GAL4^da.G32

(Willert et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^h-1J3

(Povelones and Nusse, 2005)

embryonic epidermis, with Scer\GAL4^da.G32

(Willert et al., 1999)

eye, with Scer\GAL4^ey.PH

(Ekas et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Chang et al., 2008, Fang et al., 2006, Parker et al., 2002, Penton et al., 2002, Willert et al., 1999)

histoblast | male limited, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

scutellar bristle | male limited, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

scutellar bristle | male limited | increased number, with Scer\GAL4^dsx-GAL4

(Wang et al., 2011)

ventral denticle belt, with Scer\GAL4^prd.RG1

(Hacker et al., 1997)

Detailed Description

Statement

Reference

Flies overexpressing wg^Scer\UAS.cKa under the control of Scer\GAL4^Hml.Δ in the hemocytes develop normally, with no obvious defects after they hatch. Expression of hh^Scer\UAS.cIa does not protect against infection with group B streptococcus.

(Wang et al., 2011)

Ectopic expression of wg^Scer\UAS.cKa in males under the control of Scer\GAL4^dsx-GAL4 results in the formation of a female-like adult abdominal segment 7 cuticle. The mutant males develop excess tissue posterior to the A6 tergite. This excess tissue is sclerotised and pigmented, suggesting that it adopts anterior cuticle fate. Ventral histoblasts are also transformed generating an A7 sternite. Additionally, ectopic wg^Scer\UAS.cKa expression results in partial transformation of the male A6 sternite toward more anterior identity. Compared with wild-type males, the A6 sternite of males expressing wg^Scer\UAS.cKa under the control of Scer\GAL4^dsx-GAL4 is more rectangular and develop supernumerary bristles. The transformed A7 cuticle of the mutant males is narrower than more anterior segments and devoid of bristles.

(Wang et al., 2011)

Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF causes a dramatic reduction in adult eye size.

(Chang et al., 2008)

Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^ey.PH results in a no eye phenotype.

(Ekas et al., 2006)

Expression of wg^Scer\UAS.cKa in the developing eye, under the control of Scer\GAL4^GMR.PF, results in a severe reduction in adult eye size.

(Fang et al., 2006)

Expression of wg^Scer\UAS.cKa in alternating embryonic segements, driven by Scer\GAL4^h-1J3, results in the formation of ectopic naked cuticle.

(Povelones and Nusse, 2005)

When wg^Scer\UAS.cKa is driven by Scer\GAL4^GMR.PF mutant animals have a dramatically reduced eye.

(Parker et al., 2002)

Adults expressing wg^Scer\UAS.cKa under the control of Scer\GAL4^GMR.PF have reduced eyes.

(Penton et al., 2002)

When expression is driven by Scer\GAL4^da.G32 a completely naked embryonic cuticle results. When expression is driven in the eye with Scer\GAL4^GMR.PF, a glassy reduced eye results. When expression is driven in the eye with Scer\GAL4^GMR.PF, the eye becomes slightly roughened.

(Willert et al., 1999)

Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^prd.RG1 in wild-type embryos results in deletion of denticle bands at 16^oC and 25^oC.

(Hacker et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, wg^UAS.cKa has abnormal size | adult stage phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, wg^UAS.cKa has visible | adult stage phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, wg^UAS.cKa has abnormal size | adult stage phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, spen^RNAi.UAS, wg^UAS.cKa has visible | adult stage phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, spen^RNAi.UAS, wg^UAS.cKa has abnormal size | adult stage phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, nito^RNAi.UAS.cCa, wg^UAS.cKa has visible | adult stage phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, nito^RNAi.UAS.cCa, wg^UAS.cKa has abnormal size | adult stage phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, wg^UAS.cKa has visible | adult stage phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^dsx-GAL4, wg^UAS.cKa has lethal phenotype

(Wang et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, spen^RNAi.UAS, wg^UAS.cKa has eye phenotype, suppressible | partially by nito^RNAi.UAS.cCa/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, nito^RNAi.UAS.cCa, wg^UAS.cKa has eye phenotype, suppressible | partially by spen^RNAi.UAS/Scer\GAL4^GMR.PF

(Chang et al., 2008)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible by CtBP^GSA132, Scer\GAL4^GMR.PF

(Fang et al., 2006)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible by CtBP^GSA396, Scer\GAL4^GMR.PF

(Fang et al., 2006)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible by pygo^EP1076/Scer\GAL4^GMR.PF

(Parker et al., 2002)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible by pygo^UAS.cPa/Scer\GAL4^GMR.PF

(Parker et al., 2002)

Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype, suppressible by sgg^EP1576/Scer\GAL4^GMR.PF

(Parker et al., 2002)

Suppressor of

Statement

Reference

Scer\GAL4^prd.RG1/wg^UAS.cKa is a suppressor of embryonic/first instar larval cuticle phenotype of dlp^A187

(Yan et al., 2010)

Scer\GAL4^prd.RG1/wg^UAS.cKa is a suppressor of embryonic/first instar larval cuticle phenotype of sll^7E18

(Luders et al., 2003)

Scer\GAL4^prd.RG1/wg^UAS.cKa is a suppressor of ventral denticle belt phenotype of sll^7E18

(Luders et al., 2003)

Other

Statement

Reference

Axn^A1.UAS, Scer\GAL4^GMR.PF, wg^UAS.cKa has eye phenotype

(Willert et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^prd.RG1 restores naked cuticle in dlp^A187 embryos derived from dlp^A187 female germline clones.

(Yan et al., 2010)

Expression of spen^{dsRNA.Scer\UAS} partially suppresses the reduction in eye size seen when wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^GMR.PF.

Expression of nito^{dsRNA.Scer\UAS.cCa} partially suppresses the reduction in eye size seen when wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^GMR.PF.

Co-expression of nito^{dsRNA.Scer\UAS.cCa} and spen^{dsRNA.Scer\UAS} partially suppresses the reduction in eye size seen when wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^GMR.PF. The suppression is greater than is seen when either transgene is expressed alone.

(Chang et al., 2008)

Coexpression of either CtBP^GSA132 or CtBP^GSA396, suppress the eye phenotype of Scer\GAL4^GMR.PF>wg^Scer\UAS.cKa flies.

(Fang et al., 2006)

When wg^Scer\UAS.cKa or hh^Scer\UAS.cIa is driven by Scer\GAL4^prd.RG1 in sll^7E18 animals suppression of the segment polarity phenotype is seen in those domains that express Scer\GAL4^prd.RG1.

(Luders et al., 2003)

When wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^prd.RG1 in a an embryo that is zygotically and maternally mutant for frc⁰⁰⁰⁷³, naked cuticle is restored in a typical pair-rule pattern.

(Selva et al., 2001)

When expression of Axn^A1.Scer\UAS along with wg^Scer\UAS.cKa is driven in the eye by Scer\GAL4^GMR.PF the eye phenotype is slightly reduced and roughened, i.e. intermediate between that of Axn^A1.Scer\UAS or wg^Scer\UAS.cKa alone.

(Willert et al., 1999)

Expression of wg^Scer\UAS.cKa under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos induces naked cuticle at 25^oC. Narrow regions of naked cuticle are produced when wg^Scer\UAS.cKa is expressed under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos at 16^oC.

(Hacker et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

H. Krause.

(Hacker et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

wg^Scer\UAS.cKa

wg^UAS.cKa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Krause

(Hacker et al., 1997)

Secondary FlyBase IDs

References (21)

Report Sections

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