Expression of hthScer\UAS.T:Avic\GFP under the control of Scer\GAL4tub.PU in l(2)gl4 mutant wing disc mutant clones results in overproliferation and neoplastic transformation in the proximal domain. Clones in the distal domain are mostly eliminated.
Clones of cells in the eye disc expressing both hthScer\UAS.T:Avic\GFP and tshScer\UAS.cGa under the control of Scer\GAL4αTub84B.PZ grow well in the middle of the disc and can induce large outgrowths at the edge of the disc.
Expression of both DllScer\UAS.cBa and hthScer\UAS.T:Avic\GFP under the control of Scer\GAL4dpp.blk1 results in the formation of ectopic antennal structures in the leg (in 19% of animals), such as transformation of one of the tarsal claws to arista. Extra antennal tissue is induced on the head, with as many as three ectopic antennae on each side of the head. Aristae are induced on female and male genital disc derivatives (in 43% of animals). Ectopic antennal tissue is not seen in the genital disc derivatives if hthScer\UAS.T:Avic\GFP or DllScer\UAS.cBa are expressed alone under the control of Scer\GAL4dpp.blk1. Ectopic antennal tissue is not seen in the leg if hthScer\UAS.T:Avic\GFP is expressed alone under the control of Scer\GAL4dpp.blk1, and is seen in less than 1% of animals when DllScer\UAS.cBa alone is expressed under the control of Scer\GAL4dpp.blk1.