FlyBase Allele Report: Dmel\vas[PH165]

General Information

Symbol

Dmel\vas^PH165

Species

D. melanogaster

Name

FlyBase ID

FBal0090073

Feature type

allele

Associated gene

Dmel\vas

Associated Insertion(s)

Carried in Construct

Also Known As

vasa^PH165

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Styhler et al., 1998)

Mutagen

Delta2-3 transposase

(Styhler et al., 1998)

ethyl methanesulfonate

(Ashburner et al., 1999.10.12)

Progenitor genotype

P{PZ}vas^LYG2

(Styhler et al., 1998)

Caused by aberration

Df(2L)vas^PH165

Cytology

Description

Imprecise excision of P{PZ}vas^LYG2, resulting in a 7343bp deletion which removes the entire vas coding region (the first, non-coding, exon is not deleted). This deletion also removes nested coding sequence located within the third intron of vas (FlyBase curator comment: this nested coding sequence corresponds to the coding exons specific to the solo gene).

(Styhler et al., 1998)

7343bp deletion that removes the entire coding regions of vas and solo. The deletion removes the second and third of the three exons common to vas and solo, plus all of the solo-specific and vas-specific exons (see FBrf0102626).

(FlyBase, 1996-)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:15,068,097..15,075,439 [+]

Inserted_sequence:

CATGATGAAATAACAT

Comment:

Deletion in which 7343bp are removed including the entire vas coding region and replaced with 16bp from the P element.

(Styhler et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by l(3)mbt^ts1

(Janic et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst cell of ovary

(Styhler et al., 1998)

cystoblast (with Df(2L)BSC299)

(Pek and Kai, 2011)

dorsal appendage

(Dehghani and Lasko, 2016, Johnstone and Lasko, 2004, Styhler et al., 1998)

dorsal appendage | maternal effect (with vas^RG53)

(Ferguson et al., 2012)

egg

(Dehghani and Lasko, 2016, Styhler et al., 1998)

egg chamber

(Styhler et al., 1998)

egg chorion | maternal effect (with vas^RG53)

(Ghabrial and Schupbach, 1999)

embryo | maternal effect (with vas^W660E)

(Dehghani and Lasko, 2016)

female germline stem cell

(Styhler et al., 1998)

female germline stem cell (with Df(2L)BSC299)

(Pek and Kai, 2011)

germarium region 1

(Styhler et al., 1998)

germline cyst

(Styhler et al., 1998)

male germline cell | decreased number (with vas^EP812)

(Adashev et al., 2024)

male germline stem cell | decreased number (with vas^EP812)

(Adashev et al., 2024)

micropyle

(Styhler et al., 1998)

micropyle | ectopic

(Johnstone and Lasko, 2004)

nurse cell

(Styhler et al., 1998)

oocyte

(Styhler et al., 1998)

oocyte | oogenesis stage S6

(Styhler et al., 1998)

oocyte | oogenesis stage S9

(Styhler et al., 1998)

oocyte | oogenesis stage S10

(Styhler et al., 1998)

oocyte nucleus

(Styhler et al., 1998)

ovary

(Johnstone and Lasko, 2004, Styhler et al., 1998)

primordial germ cell | maternal effect

(Johnstone and Lasko, 2004)

primordial germ cell | maternal effect (with vas^W660E)

(Dehghani and Lasko, 2016)

spermatocyte | decreased number (with vas^EP812)

(Adashev et al., 2024)

Detailed Description

Statement

Reference

vas^PH165 mutant females lay significantly fewer eggs than controls and those eggs display severe dorsal appendage defects.

Embryos from vas^W660E/vas^PH165 transheterozygous mothers fail to form pole cells or have only drastically reduced number of them. The number of eggs produced by either the females is comparable to wild-type but the eggs display frequent dorsal appendage defects.

(Dehghani and Lasko, 2016)

Eggs laid by vas^PH165/vas^RG53 females display a range of ventralisation phenotypes, ranging from fusion at the base of the dorsal appendages through to complete lack of the appendages. This phenotype can be suppressed by feeding the females rapamycin.

(Ferguson et al., 2012)

Progression of chromosome condensation appears delayed in germline stem cells (GSCs) and cystoblasts (CBs) of vas^PH165/Df(2L)BSC299 ovaries. The mutant ovaries show an increased percentage of GSCs and CBs in prometaphase compared to controls, with a concomitant drop in the number of GSCs and CBs in metaphase. 56.7% of the mutant GSCs and CBs have lagging chromosomes during anaphase.

(Pek and Kai, 2011)

vas¹/vas^PH165 egg chambers are similar to wild-type, consisting of 15 nurse cells and an oocyte surrounded by a layer of follicle cells.

(Liu et al., 2009)

vas^PH165 mutant flies produce few mature eggs. In 3-4 day old females the number of stage 14 eggs per ovary ranges widely from 0-32 with an average of nine. Most mature eggs either lack or have fused dorsal appendages, and sometimes have an ectopic micropyle at the posterior end. Mature eggs from these mothers can be fertilised, but the resulting embryos have posterior segmentation defects and do not hatch. Fewer than 1% of fertilised embryos from vas^PH165 mutant mothers have pole cells at cellular blastoderm.

(Johnstone and Lasko, 2004)

10-30% of embryos produced by heterozygous females at 29^oC fail to hatch and show variable and minor patterning defects which affect both the anteroposterior and dorsoventral axes.

(Carrera et al., 2000)

Homozygous females show defects in oogenesis. The ovaries contain fewer developing cysts than normal. This phenotype shows high expressivity and increases in severity with the age of the female. Region 1 of the germaria of 7 day old females often consists of only a few stem cells and developing cysts. Posterior to these there is often what appears to be an extended interfollicular stalk, which is probably formed by follicle cells in the absence of cystocyte clusters. Approximately 1% of homozygous ovaries have defects in germline differentiation and oocyte determination, including tumorous egg chambers, egg chambers with 16 nurse cells and no oocyte, egg chambers with two oocytes, and egg chambers with a mislocalised oocyte. The oocytes produced by homozygous egg chambers are not fully differentiated; the oocyte nucleus appears more diffuse than in wild-type females. Approximately 70% of homozygous egg chambers appear normal until about stage 6, whereupon all the nuclei undergo pycnosis and further development ceases. Approximately 20% of oocytes continue beyond stage 6, most of these are blocked at stage 9-10, after which the oocyte often loses its integrity at the anterior end, and nurse cell nuclei invade the region of the egg chamber normally occupied by the oocyte. A small number of oocytes complete oogenesis, but frequently have duplicated micropyles at both the anterior and posterior ends. These eggs also often have dorsal appendage defects; 19% lack dorsal appendages, 66% have a single fused appendage and only 15% have two dorsal appendages. Homozygotes are rescued to fertility by vas^T:Avic\GFP.

(Styhler et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

vas^PH165 has some die during embryonic stage | maternal effect phenotype, enhanceable by eIF5B^Δ1/eIF5B[+]

(Carrera et al., 2000)

vas^PH165 has some die during embryonic stage | maternal effect phenotype, enhanceable by Df(3L)G5/+

(Carrera et al., 2000)

Suppressed by

Statement

Reference

vas^PH165/Df(2L)BSC299 has abnormal mitotic cell cycle | female phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/barr^UASp.EGFP

(Pek and Kai, 2011)

NOT suppressed by

Statement

Reference

vas^PH165 has decreased fecundity | female phenotype, non-suppressible by bel::vas^{vas.C.bel.EGFP}

(Dehghani and Lasko, 2016)

Suppressor of

Statement

Reference

vas^PH165/Df(2L)A267 is a suppressor of neoplasia phenotype of l(3)mbt^ts1

(Janic et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

vas^PH165/Df(2L)BSC299 has female germline stem cell phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/barr^UASp.EGFP

(Pek and Kai, 2011)

vas^PH165/Df(2L)BSC299 has cystoblast phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/barr^UASp.EGFP

(Pek and Kai, 2011)

NOT suppressed by

Statement

Reference

vas^PH165 has egg phenotype, non-suppressible by bel::vas^{vas.C.bel.EGFP}

(Dehghani and Lasko, 2016)

vas^RG53/vas^PH165 has egg chorion | maternal effect phenotype, non-suppressible by mei-41^D3/mei-41^D3

(Ghabrial and Schupbach, 1999)

Enhancer of

Statement

Reference

vas^PH165/vas[+] is an enhancer of primordial germ cell | maternal effect phenotype of Fsn^UAS.Tag:HA, Scer\GAL4^{VP16.nanos.UTR}

(Kugler et al., 2010)

vas^PH165/vas¹ is an enhancer of oocyte phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

vas^PH165/vas¹ is an enhancer of egg chamber phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

vas^PH165/vas¹ is an enhancer of nurse cell phenotype of mei-P26^fs1/mei-P26¹

(Liu et al., 2009)

Other

Statement

Reference

eIF5B^Δ1, vas^PH165 has embryonic abdominal segment 4 phenotype

(Carrera et al., 2000)

eIF5B^Δ1, vas^PH165 has primordial germ cell phenotype

(Carrera et al., 2000)

eIF5B^Δ1/eIF5B[+], vas^PH165 has embryonic abdominal segment 4 phenotype

(Carrera et al., 2000)

eIF5B^Δ1/eIF5B[+], vas^PH165 has primordial germ cell phenotype

(Carrera et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The very low number of eggs laid by vas^PH165 homozygous females is not rescued by maternal combination with bel::vas^{vas.C.bel.T:Avic\GFP-EGFP} and the few embryos produced by the vas^PH165;bel::vas^{vas.C.bel.T:Avic\GFP-EGFP} females display severe defects in dorsal-ventral patterning.

(Dehghani and Lasko, 2016)

The delay in prometaphase and the chromosome segregation defects which are seen in the germline stem cells and cystoblasts of vas^PH165/Df(2L)BSC299 ovaries are rescued by expression of barr^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Pek and Kai, 2011)

The frequency with which l(3)mbt^ts1 larval brain tissue develops into tumours after allograft into adult host flies (taking over the entire abdomen of the host) is markedly reduced if the transplanted tissue is also carrying vas^PH165/Df(2L)A267.

(Janic et al., 2010)

vas^PH165/+ enhances the primordial germ cell phenotype resulting from the overexpression of Scer\GAL4^{nos.UTR.T:Hsim\VP16}>Fsn^{Scer\UAS.T:Ivir\HA1}.

(Kugler et al., 2010)

More than 50% of vas¹/vas^PH165 ; mei-P26¹/mei-P26^fs1 egg chambers are tumorous and fail to differentiate nurse cells and oocytes. The double mutant egg chambers contain mostly early-stage cystocytes as they contain either punctate spectrosomes or branched fusomes.

(Liu et al., 2009)

The frequency of unhatched embryos produced by vas^PH165/+ females at 29^oC is increased by addition of one copy of eIF5B^Δ1 or Df(3L)G5. Many embryos from vas^PH165/+ ; eIF5B^Δ1/+ mothers show severe segmentation defects and 26% show a vas-like phenotype in which the 4th abdominal segment is completely or partially deleted (partial or total deletion of A4 is never seen in the progeny of either single heterozygote). There is a marked reduction in the number of pole cells in embryos derived from double heterozygotes, and occasionally pole cells are completely absent. The pole cells are often interspersed with or positioned beneath somatic cells. No reduction in pole cell number is seen in the progeny of either vas^PH165/+ ; eIF-4a⁰²⁴³⁹/+, vas^PH165/+ ; eIF-4a¹⁰¹³/+ or vas^PH165/+ ; eIF-4E^S058911/+ females.

(Carrera et al., 2000)

The eggshell patterning defects of eggs derived from vas^RG53/vas^PH165 females are not suppressed if the females also carry mei-41^D3/mei-41^D3.

(Ghabrial and Schupbach, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

vas^PH165 is rescued by vas^EGFP

(Pek and Kai, 2011)

vas^PH165/vas¹ is rescued by vas^cKa.GFP

(Kugler et al., 2010)

vas^PH165/vas¹ is rescued by vas^5xAla.GFP

(Kugler et al., 2010)

vas^PH165 is rescued by vas^EGFP

(Johnstone and Lasko, 2004)

vas^PH165 is rescued by vas^GFP

(Styhler et al., 1998)

Partially rescued by

vas^PH165 is partially rescued by vas^Δ15-75.EGFP

(Dehghani and Lasko, 2016)

vas^PH165 is partially rescued by vas^W660E.EGFP

(Dehghani and Lasko, 2016)

vas^PH165 is partially rescued by vas^W660F.EGFP

(Dehghani and Lasko, 2016)

vas^PH165 is partially rescued by vas^Δ617.EGFP

(Johnstone and Lasko, 2004)

Comments

The reduced number of eggs laid by vas^PH165 homozygous females as well as the dorsal appendage defects in the eggs produced are strongly improved by maternal combination with either vas^{Δ15-75.T:Avic\GFP-EGFP} or vas^{W660F.T:Avic\GFP-EGFP} and to a lesser degree by vas^{W660E.T:Avic\GFP-EGFP}.

(Dehghani and Lasko, 2016)

The mitotic delay which is seen in the germline stem cells and cystoblasts of vas^PH165 ovaries is rescued by vas^{T:Avic\GFP-EGFP} and by vas^{Δ617.T:Avic\GFP-EGFP}.

(Pek and Kai, 2011)

Maternal expression of vas^{5xAla.T:Avic\GFP} rescues the viability and primordial germ cell formation defect in vas¹/vas^PH165 embryos.

Maternal expression of vas^{cKa.T:Avic\GFP} rescues the viability and primordial germ cell formation defect in vas¹/vas^PH165 embryos.

(Kugler et al., 2010)

vas^{T:Avic\GFP-EGFP} rescued the oogenesis defects and the maternal effect embryonic defects due to vas^PH165. vas^{Δ617.T:Avic\GFP-EGFP} does not rescue the oogenesis defects or the maternal effect elimination of pole cells in the embryo due to vas^PH165. However, posterior segmentation defects are partly rescued, and embryos hatch into viable larvae.

(Johnstone and Lasko, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

vas^PH

(Dold et al., 2020)

vasa^PH165

(Lim et al., 2022, Tsai et al., 2014, Pek and Kai, 2011, Janic et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (36)

Report Sections

Open Close