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General Information
Symbol
Dmel\hiwND9
Species
D. melanogaster
Name
FlyBase ID
FBal0118195
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    A15054391T

    Amino acid change:

    K2001term | hiw-PA; K2001term | hiw-PB

    Reported amino acid change:

    W2001term

    Comment:

    The site of the mutation was inferred by the curator based on the author report of a mutation at position 2001.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: W2001term.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    hiwND9 third instar larvae exhibit dramatic overgrowth of the neuromuscular junction, namely increased branching and increased number of presynaptic boutons, as compared to controls.

    hiwND9 mutants display significantly enhanced aversive long term memory after one-session or two-session spaced training, but show normal long term memory after ten-session spaced training. Sensorimotor abilities are unchanged compared with controls. hiwND9 mutants show a normal memory three hours after training and a slightly but not significantly elevated memory at 8 hours, whereas they exhibit significantly enhanced memory at the 12 hours and 24 hour time points compared with controls. Short term and intermediate term memory are similar to controls.

    The enhancement of aversive long-term memory seen in hiwND9 mutant flies is eliminated when the flies are fed the protein synthesis inhibitor cycloheximide.

    Mutant larvae show synaptic terminal overgrowth at the neuromuscular junction, with an increase in bouton number compared to wild type.

    Homozygotes or hemizygotes over Df(1)RK2 or Df(1)RK4 all have synapses that are longer and more branched with more numerous but smaller boutons.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The synaptic terminal overgrowth phenotype seen at the neuromuscular junction is not significantly different in hiwND9 Fsnf06595 double mutant larvae compared to hiwND9 single mutant larvae.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    Expression of hiwScer\UAS.fl under the control of Scer\GAL4elav.Switch.PO (in the presence of RU486) rescues the aversive long term memory defects seen in hiwND9 mutant flies.

    Expression of hiwΔRING.Scer\UAS under the control of Scer\GAL4elav.Switch.PO (in the presence of RU486) does not rescue or further enhance the aversive long term memory defects seen in hiwND9 mutant flies.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    References (10)