FlyBase Allele Report: Dmel\hiw[ND9]

General Information

Symbol

Dmel\hiw^ND9

Species

D. melanogaster

Name

FlyBase ID

FBal0118195

Feature type

allele

Associated gene

Dmel\hiw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Wan et al., 2000)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: W2001term.

(Wan et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:15,054,391..15,054,391 [-]

Nucleotide change:

A15054391T

Amino acid change:

K2001term | hiw-PA; K2001term | hiw-PB

Reported amino acid change:

W2001term

Comment:

The site of the mutation was inferred by the curator based on the author report of a mutation at position 2001.

(Wan et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction

(West et al., 2018, Wu et al., 2007)

embryonic/larval neuromuscular junction | third instar larval stage

(Russo and DiAntonio, 2019)

NMJ bouton | increased number

(Wu et al., 2007)

NMJ bouton | increased number | larval stage

(West et al., 2018)

NMJ bouton | increased number | third instar larval stage

(Russo and DiAntonio, 2019)

synapse

(Wan et al., 2000)

Detailed Description

Statement

Reference

hiw^ND9 third instar larvae exhibit dramatic overgrowth of the neuromuscular junction, namely increased branching and increased number of presynaptic boutons, as compared to controls.

(Russo and DiAntonio, 2019)

hiw^ND9 mutants display significantly enhanced aversive long term memory after one-session or two-session spaced training, but show normal long term memory after ten-session spaced training. Sensorimotor abilities are unchanged compared with controls. hiw^ND9 mutants show a normal memory three hours after training and a slightly but not significantly elevated memory at 8 hours, whereas they exhibit significantly enhanced memory at the 12 hours and 24 hour time points compared with controls. Short term and intermediate term memory are similar to controls.

The enhancement of aversive long-term memory seen in hiw^ND9 mutant flies is eliminated when the flies are fed the protein synthesis inhibitor cycloheximide.

(Huang et al., 2012)

Mutant larvae show synaptic terminal overgrowth at the neuromuscular junction, with an increase in bouton number compared to wild type.

(Wu et al., 2007)

Homozygotes or hemizygotes over Df(1)RK2 or Df(1)RK4 all have synapses that are longer and more branched with more numerous but smaller boutons.

(Wan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

hiw^ND9 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND9 has abnormal size | third instar larval stage phenotype, suppressible by wnd^GL00282/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND9 has abnormal neuroanatomy | recessive | larval stage phenotype, suppressible by lace^k05305/lace^k05305

(West et al., 2018)

Suppressor of

Statement

Reference

hiw^ND9/hiw^ND9 is a suppressor of abnormal neuroanatomy | larval stage phenotype of lace^k05305

(West et al., 2018)

Other

Statement

Reference

hiw^ND9, lace^k05305 has abnormal neuroanatomy | dominant | larval stage phenotype

(West et al., 2018)

hiw^ND9, lace^k05305 has abnormal neuroanatomy | larval stage phenotype

(West et al., 2018)

hiw^ND9, lace^k05305 has increased size | larval stage phenotype

(West et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

hiw^ND9 has NMJ bouton | increased number | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND9 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Fmr1^UAS.cZa/Scer\GAL4^elav.PU

(Russo and DiAntonio, 2019)

hiw^ND9 has NMJ bouton | increased number | larval stage phenotype, suppressible by lace^k05305/lace^k05305

(West et al., 2018)

Suppressor of

Statement

Reference

hiw^ND9/hiw^ND9 is a suppressor of NMJ bouton | larval stage | decreased number phenotype of lace^k05305

(West et al., 2018)

Other

Statement

Reference

hiw^ND9, lace^k05305 has NMJ bouton | larval stage | decreased number phenotype

(West et al., 2018)

hiw^ND9, lace^k05305 has embryonic/larval neuromuscular junction phenotype

(West et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The synaptic terminal overgrowth phenotype seen at the neuromuscular junction is not significantly different in hiw^ND9 Fsn^f06595 double mutant larvae compared to hiw^ND9 single mutant larvae.

(Wu et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

hiw^ND9 is rescued by Scer\GAL4^{elav.Switch.PO}/hiw^UAS.fl

(Huang et al., 2012)

Not rescued by

hiw^ND9 is not rescued by Scer\GAL4^{elav.Switch.PO}/hiw^ΔRING.UAS

(Huang et al., 2012)

Comments

Expression of hiw^Scer\UAS.fl under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) rescues the aversive long term memory defects seen in hiw^ND9 mutant flies.

Expression of hiw^{ΔRING.Scer\UAS} under the control of Scer\GAL4^{elav.Switch.PO} (in the presence of RU486) does not rescue or further enhance the aversive long term memory defects seen in hiw^ND9 mutant flies.

(Huang et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hiw^ND9

(West et al., 2015)

ND9

(Wu et al., 2005)

hiw^ND9

(Ugbode et al., 2020, Russo and DiAntonio, 2019, West et al., 2018, Huang et al., 2012, Milton et al., 2011, Zhao et al., 2009, Wu et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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