FlyBase Allele Report: Dmel\rl[Sem.C.UAS]

General Information

Symbol

Dmel\rl^Sem.C.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0124961

Feature type

allele

Associated gene

Dmel\rl

Associated Insertion(s)

Carried in Construct

P{UAS-rl^Sem}

Also Known As

UAS-rolled^SEM, UAS-rl^ACT

Key Links

Transgenic product class

missense_variant

(Ciapponi et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Ciapponi et al., 2001)

Mutagen

in vitro construct

(Ciapponi et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-rl^Sem}

Cytology

Description

UASt sequences drive expression of rl^Sem.

(Ciapponi et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate neurodegenerative disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Liévens et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant, with Scer\GAL4^elav-C155

(Eddison et al., 2011, Corl et al., 2009)

increased cell size | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

short lived, with Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

visible, with Scer\GAL4^32B

(Gritzan et al., 2002)

visible, with Scer\GAL4^Bx-MS1096

(Lee et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

visible | adult stage, with Scer\GAL4^GMR.PU

(Liévens et al., 2008)

Phenotype Manifest In

adult gut, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

enteroblast | adult stage, with Scer\GAL4^Su(H).GBE

(Rojas Villa et al., 2019)

eye, with Scer\GAL4^GMR.PF

(Corl et al., 2009)

eye, with Scer\GAL4^GMR.PU

(Liévens et al., 2008)

wing, with Scer\GAL4^32B

(Gritzan et al., 2002)

wing, with Scer\GAL4^Bx-MS1096

(Lee et al., 2003)

wing vein | ectopic, with Scer\GAL4^32B

(Gritzan et al., 2002, Ciapponi et al., 2001)

Detailed Description

Statement

Reference

Expression of rl^Sem.C.UAS under the control of Scer\GAL4^Su(H).GBE (in combination with tub-Gal80[ts] to restrict expression to the adult stage) leads to an increase in the cell size of enteroblasts in adult guts when compared to controls.

(Rojas Villa et al., 2019)

Neuronal expression of rl^{Sem.C.Scer\UAS} (under the control of Scer\GAL4^elav-C155) reduces ethanol sensitivity.

Neuronal expression of rl^{Sem.C.Scer\UAS} (under the control of Scer\GAL4^elav-C155) throughout development, until the adult stage (where Scer\GAL80^ts.αTub84B is induced) does not reduce ethanol sensitivity. Neuronal expression only after eclosion of the adult fly also fails to reduce ethanol sensitivity.

(Eddison et al., 2011)

Expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^elav-C155 results in flies with increased resistance to ethanol-induced sedation compared to control flies.

Expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Corl et al., 2009)

Expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^GMR.PU produces a rough eye phenotype.

Expression of rl^{Sem.C.Scer\UAS} has no effect on lifespan when expressed under the control of Scer\GAL4^elav-C155 or Scer\GAL4^repo.PU. A reduction in lifespan is seen when rl^{Sem.C.Scer\UAS} is expressed under the control of Scer\GAL4^Eaat1.PR, but not until 24 days post-eclosion.

(Liévens et al., 2008)

20.9% of flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 have blistered wings.

(Lee et al., 2003)

Expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^32B results in ectopic vein material in the wing. The flies do not have a held out wing phenotype.

(Gritzan et al., 2002)

When driven by Scer\GAL4^32B, causes an extra vein phenotype which is marked at 25^oC and milder at 18^oC.

(Ciapponi et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, rl^Sem.C.UAS has visible phenotype, non-enhanceable by by²/by[+]

(Lee et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, rl^Sem.C.UAS has chemical resistant phenotype, suppressible by aru^8.128

(Eddison et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rl^Sem.C.UAS has visible phenotype, non-suppressible by hppy^RB.UAS/Scer\GAL4^GMR.PF

(Corl et al., 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/rl^Sem.C.UAS is a non-suppressor of chemical sensitive phenotype of aru^8.128

(Eddison et al., 2011)

rl^Sem.C.UAS, Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color | progressive phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^repo.PU is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^repo.PU

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^Eaat1.PR is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^elav-C155 is a non-suppressor of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Liévens et al., 2008)

Other

Statement

Reference

Scer\GAL4^32B, eg^18B, rl^Sem.C.UAS has visible phenotype

(Gritzan et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, enhanceable by eg^18B

(Gritzan et al., 2002)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, enhanceable by svp¹

(Gritzan et al., 2002)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, enhanceable by tll¹

(Gritzan et al., 2002)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, enhanceable by Scer\GAL4^32B/kay^UAS.cEa

(Ciapponi et al., 2001)

NOT Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, rl^Sem.C.UAS has wing phenotype, non-enhanceable by by²/by[+]

(Lee et al., 2003)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, non-enhanceable by kni²

(Gritzan et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, suppressible by kay²

(Gritzan et al., 2002)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, suppressible by Scer\GAL4^32B/kay^Fbz.UAS

(Ciapponi et al., 2001)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rl^Sem.C.UAS has eye phenotype, non-suppressible by hppy^RB.UAS/Scer\GAL4^GMR.PF

(Corl et al., 2009)

Scer\GAL4^32B, rl^Sem.C.UAS has wing vein | ectopic phenotype, non-suppressible by kni²

(Gritzan et al., 2002)

NOT Suppressor of

Statement

Reference

rl^Sem.C.UAS, Scer\GAL4^elav-C155 is a non-suppressor of adult mushroom body gamma-lobe phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^elav-C155

(Liévens et al., 2008)

rl^Sem.C.UAS, Scer\GAL4^GMR.PU is a non-suppressor of eye | adult stage phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^GMR.PU

(Liévens et al., 2008)

Other

Statement

Reference

Scer\GAL4^32B, eg^18B, rl^Sem.C.UAS has wing phenotype

(Gritzan et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Flies over-expressing rl^{Sem.C.Scer\UAS} in neurons with Scer\GAL4^elav-C155 in a aru^8.128 background shows increased sensitivity to ethanol sedation that is not significantly different from that of aru^8.128 flies, indicating that a aru^8.128 background suppresses the decreased ethanol sensitivity caused by overexpression of rl^{Sem.C.Scer\UAS}.

(Eddison et al., 2011)

Co-expression of hppy^RB.Scer\UAS has no effect on the rough eye phenotype caused by expression of rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^GMR.PF.

(Corl et al., 2009)

The wing blister phenotype of flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 is not significantly enhanced by by²/+.

(Lee et al., 2003)

eg^18B flies expressing rl^{Sem.C.Scer\UAS} under the control of Scer\GAL4^32B have a held out wing phenotype.

(Gritzan et al., 2002)

Xenogenetic Interactions

Statement

Reference

Expression of rl^{Sem.C.Scer\UAS} does not suppress the pigment cell degeneration seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of rl^{Sem.C.Scer\UAS} does not suppress the γ-lobe kenyon cell degeneration seen in the mushroom bodies when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of Scer\GAL4^elav-C155.

Expression of rl^{Sem.C.Scer\UAS} does not suppress the reduction in lifespan seen when Hsap\HTT^{Q93.ex1p.Scer\UAS} is expressed under the control of any of Scer\GAL4^elav-C155, Scer\GAL4^repo.PU or Scer\GAL4^Eaat1.PR.

(Liévens et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Erk^act

(Lievens et al., 2005)

rl^{Sem.C.Scer\UAS}

rl^Sem.C.UAS

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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