FlyBase Allele Report: Dmel\Liprin-α[R60]

General Information

Symbol

Dmel\Liprin-α^R60

Species

D. melanogaster

Name

FlyBase ID

FBal0137466

Feature type

allele

Associated gene

Dmel\Liprin-α

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Kaufmann et al., 2002)

Progenitor genotype

P{EP}homer^EP2141

(Kaufmann et al., 2002)

Caused by aberration

Df(2L)Liprin-α^R60

Cytology

Description

Imprecise excision of P{EP}EP2141, removing most of the upstream region of Liprin-α.

(Kaufmann et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Liprin-α^F3ex15)

(Kaufmann et al., 2002)

abnormal neuroanatomy | adult stage | somatic clone

(Holbrook et al., 2012)

abnormal neuroanatomy | somatic clone

(Choe et al., 2006)

abnormal neuroanatomy | third instar larval stage (with Liprin-α^F3ex15)

(Owald et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The third instar larval neuromuscular junction of Liprin-α^F3ex15/Liprin-α^R60 transheterozygotes exhibit a significant decrease in active zone density (identified by Bruchpilot puncta), as compared to controls.

(Böhme et al., 2016)

Liprin-α^R60 mutant larvae exhibit a vesicle localization phenotype, with ectopic aggregates of VGlut present at distal axon regions just proximal to the synaptic nerve terminal. This ectopic accumulation near synaptic termini occurs early in synaptic development and is not attributable to a gradual accumulation of synaptic vesicles during synaptic terminal growth.

(Li et al., 2014)

Axon terminals of homozygous R7 photoreceptor cell clones often fail to contact the M6 layer of the medulla.

(Holbrook et al., 2012)

Liprin-α^F3ex15/Liprin-α^R60 adults do not show defects in locomotion (assayed both by walking ability on a flat surface and in a negative geotaxis assay).

Evoked excitatory junctional currents (EJCs) are significantly reduced in amplitude in Liprin-α^F3ex15/Liprin-α^R60 third instar larvae compared to controls, while the amplitude of spontaneous miniature EJCs is normal.

The overall size of the neuromuscular junction is reduced in Liprin-α^F3ex15/Liprin-α^R60 third instar larvae compared to controls.

(Owald et al., 2010)

Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes.

(Dickman and Davis, 2009)

Liprin-α^R60 mutants exhibit a specific pattern of disruption in the structure of the cartridge, the synaptic unit in the lamina. Some cartridges have either >6 or <6 R cells axons, and some adjacent cartridges fuse.

R-cells proliferate normally during the third instar larval stage in Liprin-α^R60 eye-specific mosaics and display normal morphological differentiation during pupal and adult stages. Liprin-α^R60 mutant R cell axons select appropriate ganglion-specific targets in the lamina and the medulla and induce appropriate neuronal differentiation in the lamina target field. These axons also elaborate topographically appropriate maps in each region, with glial cell differentiation in these areas also appearing largely normal.

The behaviour of Liprin-α^R60 mutant axons (generated through MARCM) is indistinguishable from wild-type along their trajectories into the lamina plexus, with each axon remaining tightly associated with the axon bundle of its wild-type neighbours from the same ommatidium. However, once within the lamina plexus, unlike wild-type R cells, Liprin-α^R60 mutant R cells typically display specific defects in axon extension toward their targets. Two types of defect are observed. Approximately 64% of Liprin-α^R60 mutant axons completely fail to extend away from the ommatidial bundle, whereas 21% make weak, morphologically abnormal extensions; the remaining axons extend normally. All R cell subtypes are equally affected.

(Choe et al., 2006)

Liprin-α^F3ex15/Liprin-α^R60 larval neuromuscular junctions frequently lack small nascent boutons ("buds") surrounding a large end bouton (which are seen in wild-type animals).

(Kaufmann et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Liprin-α^R60/Liprin-α^F3ex15 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Nlg1^{Δcyto.UAS.EGFP}, Scer\GAL4^Mef2.PR

(Owald et al., 2012)

Other

Statement

Reference

Liprin-α^R60, RhoGAP100F^w46 has abnormal neuroanatomy | somatic clone | adult stage phenotype

(Holbrook et al., 2012)

Df(3R)RhoGAP100F^ex1.2/Df(3R)RhoGAP100F^ex3.4, Liprin-α^R60/Liprin-α^F3ex15 has lethal | embryonic stage phenotype

(Owald et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Liprin-α^R60 has synaptic vesicle phenotype, suppressible by wrd^UAS.EGFP/Scer\GAL4^Toll-6-D42

(Li et al., 2014)

Liprin-α^R60 has synapse phenotype, suppressible by wrd^UAS.EGFP/Scer\GAL4^Toll-6-D42

(Li et al., 2014)

Liprin-α^R60/Liprin-α^F3ex15 has synaptic vesicle phenotype, suppressible by Scer\GAL4^BG380/sgg^A81T.UAS

(Li et al., 2014)

Liprin-α^R60/Liprin-α^F3ex15 has synapse phenotype, suppressible by Scer\GAL4^BG380/sgg^A81T.UAS

(Li et al., 2014)

NOT Suppressor of

Statement

Reference

Liprin-α^R60/Liprin-α^F3ex15 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Nlg1^{Δcyto.UAS.EGFP}, Scer\GAL4^Mef2.PR

(Owald et al., 2012)

Other

Statement

Reference

Liprin-α^R60, RhoGAP100F^w46 has retina | somatic clone phenotype

(Holbrook et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Neuronal expression of PP2A-B'^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^D42 substantially clears VGlut accumulation at the distal axons of Liprin-α^R60 mutants.

Expression of dominant negative sgg^{A81T.Scer\UAS} under the control of Scer\GAL4^BG380 suppresses the Liprin-α^R60/Liprin-α^F3ex15 mutant distal axon phenotype.

(Li et al., 2014)

Retinas double mutant for Liprin-α^R60 and RhoGAP100F^w46 are severely reduced in size, and the remaining photoreceptor neuron axons in the medulla are extremely disorganised.

(Holbrook et al., 2012)

The reduction in bouton number per muscle area seen in third instar larvae expressing Nlg1^{Δcyto.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mef2.PR is not suppressed by Liprin-α^R60/Liprin-α^F3ex15.

(Owald et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Liprin-α^R60 is rescued by Scer\GAL4^elav.PU/Liprin-α^UAS.GFP

(Li et al., 2014)

Comments

Neuronal expression of Liprin-α^{Scer\UAS.T:Zzzz\TAP} under the control of Scer\GAL4^elav.PU completely rescues the active zone morphological defects found in Liprin-α^R60 mutants.

(Li et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8561

w^*; Df(2L)Liprin-α^R60, Liprin-α^R60/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DLiprin-α^EPexR60

(Owald et al., 2010)

Liprin-α^EPexR60

(Owald et al., 2012)

Liprin-α^R60

(Li et al., 2017, Böhme et al., 2016, Holbrook et al., 2012)

dliprin-α^R60

(Fouquet et al., 2009)

lip-α^R60

(Johnson et al., 2009)

liprin-α^EPexR60

(Li et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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