FlyBase Allele Report: Dmel\Liprin-α[F3ex15]

General Information

Symbol

Dmel\Liprin-α^F3ex15

Species

D. melanogaster

Name

FlyBase ID

FBal0137468

Feature type

allele

Associated gene

Dmel\Liprin-α

Associated Insertion(s)

P{?lacW}Liprin-α^F3ex15

Carried in Construct

Also Known As

dliprin-α^F3ex15

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kaufmann et al., 2002)

Mutagen

P-element activity

(Kaufmann et al., 2002)

Progenitor genotype

P{lacW}Liprin-α^F3

(Kaufmann et al., 2002)

Associated Insertion(s)

P{?lacW}Liprin-α^F3ex15

Cytology

Description

Imprecise excision of the P{lacW} element, removing the Liprin-α coding region. Some of the Liprin-α 5' UTR and some P-element sequences are still present.

(Kaufmann et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Liprin-α^R60)

(Kaufmann et al., 2002)

abnormal neuroanatomy | third instar larval stage (with Liprin-α^R60)

(Owald et al., 2010)

abnormal neurophysiology (with Liprin-α^1J-0)

(Miller et al., 2005)

abnormal neurophysiology (with Liprin-α^R117)

(Kaufmann et al., 2002)

majority die during embryonic stage (with Df(2L)Exel7027)

(Paul et al., 2024)

partially lethal - majority die (with Df(2L)Exel7027)

(Paul et al., 2024)

short lived (with Df(2L)Exel7027)

(Paul et al., 2024)

Phenotype Manifest In

bouton (with Liprin-α^R60)

(Kaufmann et al., 2002)

embryonic/larval neuromuscular junction | third instar larval stage (with Liprin-α^R60)

(Owald et al., 2010)

neuromuscular junction (with Liprin-α^R60)

(Kaufmann et al., 2002)

neuromuscular junction (with Liprin-α^R117)

(Kaufmann et al., 2002)

presynaptic active zone | third instar larval stage (with Liprin-α^R60)

(Böhme et al., 2016)

synapse

(Li et al., 2014)

synapse (with Liprin-α^R60)

(Li et al., 2014)

synaptic vesicle

(Li et al., 2014)

synaptic vesicle (with Liprin-α^1J-0)

(Miller et al., 2005)

synaptic vesicle (with Liprin-α^R60)

(Li et al., 2014)

Detailed Description

Statement

Reference

The third instar larval neuromuscular junction of Liprin-α^F3ex15/Liprin-α^R60 transheterozygotes exhibit a significant decrease in active zone density (identified by Bruchpilot puncta), as compared to controls.

(Böhme et al., 2016)

Liprin-α^F3ex15 mutant larvae exhibit a vesicle localization phenotype, with ectopic aggregates of VGlut present at distal axon regions just proximal to the synaptic nerve terminal. This ectopic accumulation near synaptic termini occurs early in synaptic development and is not attributable to a gradual accumulation of synaptic vesicles during synaptic terminal growth.

(Li et al., 2014)

Liprin-α^F3ex15/Liprin-α^R60 adults do not show defects in locomotion (assayed both by walking ability on a flat surface and in a negative geotaxis assay).

Evoked excitatory junctional currents (EJCs) are significantly reduced in amplitude in Liprin-α^F3ex15/Liprin-α^R60 third instar larvae compared to controls, while the amplitude of spontaneous miniature EJCs is normal.

The overall size of the neuromuscular junction is reduced in Liprin-α^F3ex15/Liprin-α^R60 third instar larvae compared to controls.

(Owald et al., 2010)

Mutant neuromuscular junctions show reliable homeostatic compensation (increase in quantal content) after treatment with philanthotoxin-433 for 10 minutes.

(Dickman and Davis, 2009)

Liprin-α^F3ex15/Liprin-α^1J-0 mutant peripheral nerves have an accumulation of clear-core vesicles along the axon, compared to wild-type axons. Visualization of synaptic vesicle precursor transport along motor axons in intact third instar larva shows that the flux of cargo in the anterograde direction is significantly decreased in the mutants, while the flux is increased in the retrograde direction. However, the total flux is unchanged. There are no ectopic presynaptic specializations, breakdowns in the plasma membrane, or obvious changes in microtubule density or morphology along motor axons of Liprin-α^F3ex15/Liprin-α^1J-0 mutants.

(Miller et al., 2005)

Liprin-α^F3ex15/Df(2L)Liprin-α-77ex63 embryos do not show defects in motor axon pathfinding. Liprin-α^F3ex15/Liprin-α^R60 larval neuromuscular junctions frequently lack small nascent boutons ("buds") surrounding a large end bouton (which are seen in wild-type animals). Overall neuromuscular junction ultrastructure looks relatively normal in Liprin-α^F3ex15/Liprin-α^R60 larvae, but active zones are always abnormal in either total size or shape, with the mean maximum dimension being nearly double that of wild type. Excitatory junctional potentials (EJPs) are reduced 36% in Liprin-α^F3ex15/Liprin-α^R117 larvae compared to controls. The mean amplitude and the frequency of unitary mRJPs is normal. The resting potential of the muscle is normal. The quantal content of evoked release is reduced by over 50%.

(Kaufmann et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(2L)Exel7027/Liprin-α^F3ex15 has increased mortality during development phenotype, suppressible | partially by Hsap\PPFIA3^UAS.Tag:HA/Scer\GAL4^da.PU

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15 has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\PPFIA3^{W546C.UAS.Tag:HA}

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15 has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^da.PU/Hsap\PPFIA3^{R784W.UAS.Tag:HA}

(Paul et al., 2024)

NOT Suppressor of

Statement

Reference

Liprin-α^R60/Liprin-α^F3ex15 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Nlg1^{Δcyto.UAS.EGFP}, Scer\GAL4^Mef2.PR

(Owald et al., 2012)

Other

Statement

Reference

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R39C.UAS.Tag:HA}, Scer\GAL4^da.PU has lethal phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R784W.UAS.Tag:HA}, Scer\GAL4^da.PU has abnormal neuroanatomy | larval stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R784W.UAS.Tag:HA}, Scer\GAL4^da.PU has short lived phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R784W.UAS.Tag:HA}, Scer\GAL4^da.PU has partially lethal phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^UAS.Tag:HA, Scer\GAL4^da.PU has abnormal neuroanatomy | larval stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^UAS.Tag:HA, Scer\GAL4^da.PU has partially lethal phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{W546C.UAS.Tag:HA}, Scer\GAL4^da.PU has abnormal neuroanatomy | larval stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{W546C.UAS.Tag:HA}, Scer\GAL4^da.PU has partially lethal phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R39C.UAS.Tag:HA}, Scer\GAL4^da.PU has abnormal neuroanatomy | larval stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R39C.UAS.Tag:HA}, Scer\GAL4^da.PU has majority die during embryonic stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R415W.UAS.Tag:HA}, Scer\GAL4^da.PU has partially lethal - majority die phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R415W.UAS.Tag:HA}, Scer\GAL4^da.PU has abnormal neuroanatomy | larval stage phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R415W.UAS.Tag:HA}, Scer\GAL4^da.PU has short lived phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R415W.UAS.Tag:HA}, Scer\GAL4^da.PU has majority die during embryonic stage phenotype

(Paul et al., 2024)

Df(3R)RhoGAP100F^ex1.2/Df(3R)RhoGAP100F^ex3.4, Liprin-α^R60/Liprin-α^F3ex15 has lethal | embryonic stage phenotype

(Owald et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Liprin-α^R60/Liprin-α^F3ex15 has synaptic vesicle phenotype, suppressible by Scer\GAL4^BG380/sgg^A81T.UAS

(Li et al., 2014)

Liprin-α^R60/Liprin-α^F3ex15 has synapse phenotype, suppressible by Scer\GAL4^BG380/sgg^A81T.UAS

(Li et al., 2014)

Liprin-α^F3ex15 has synaptic vesicle phenotype, suppressible by wrd^UAS.EGFP/Scer\GAL4^Toll-6-D42

(Li et al., 2014)

Liprin-α^F3ex15 has synapse phenotype, suppressible by wrd^UAS.EGFP/Scer\GAL4^Toll-6-D42

(Li et al., 2014)

NOT Suppressor of

Statement

Reference

Liprin-α^R60/Liprin-α^F3ex15 is a non-suppressor of NMJ bouton | third instar larval stage phenotype of Nlg1^{Δcyto.UAS.EGFP}, Scer\GAL4^Mef2.PR

(Owald et al., 2012)

Other

Statement

Reference

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R784W.UAS.Tag:HA}, Scer\GAL4^da.PU has NMJ bouton | larval stage | decreased number phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^UAS.Tag:HA, Scer\GAL4^da.PU has NMJ bouton | larval stage | decreased number phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{W546C.UAS.Tag:HA}, Scer\GAL4^da.PU has NMJ bouton | larval stage | decreased number phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R39C.UAS.Tag:HA}, Scer\GAL4^da.PU has NMJ bouton | larval stage | decreased number phenotype

(Paul et al., 2024)

Df(2L)Exel7027/Liprin-α^F3ex15, Hsap\PPFIA3^{R415W.UAS.Tag:HA}, Scer\GAL4^da.PU has NMJ bouton | larval stage | decreased number phenotype

(Paul et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

Neuronal expression of PP2A-B'^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^D42 substantially clears VGlut accumulation at the distal axons of Liprin-α^F3ex15 mutants.

Expression of dominant negative sgg^{A81T.Scer\UAS} under the control of Scer\GAL4^BG380 suppresses the Liprin-α^R60/Liprin-α^F3ex15 mutant distal axon phenotype.

(Li et al., 2014)

The reduction in bouton number per muscle area seen in third instar larvae expressing Nlg1^{Δcyto.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Mef2.PR is not suppressed by Liprin-α^R60/Liprin-α^F3ex15.

(Owald et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Liprin-α^F3ex15 is rescued by Scer\GAL4^elav.PU/Liprin-α^UAS.GFP

(Li et al., 2014)

Comments

Neuronal expression of Liprin-α^{Scer\UAS.T:Zzzz\TAP} under the control of Scer\GAL4^elav.PU completely rescues the active zone morphological defects found in Liprin-α^F3ex15 mutants.

(Li et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8563

Liprin-α^F3ex15/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

DLiprin-α^F3ex15

(Owald et al., 2010)

Liprin-α^F3ex15

(Li et al., 2017, Böhme et al., 2016, Owald et al., 2012)

dliprin-α^F3ex15

(Fouquet et al., 2009)

lip-α^F3ex5

(Johnson et al., 2009)

liprin-α^F3ex15

(Li et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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