W470term | tum-PA
G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.
embryonic epidermis & nucleus
RacGAP50CAR2 reduces the severity of the mutant phenotype seen in wgl-12 embryos at the restrictive temperature; the double mutant embryos are larger than single mutant wgl-12 embryos, and there is an increase in spacing between denticles in the double mutant embryos. Some naked cuticle is restored in wgPE2 RacGAP50CAR2 double mutant embryos. The excess naked cuticle phenotype seen in nkd2 embryos is enhanced by RacGAP50CAR2; the double mutant embryos have uniform naked cuticle with no denticle belts at all. The ability of wgl-17/+ to suppress the excess naked cuticle phenotype of nkd2 embryos is suppressed by RacGAP50CAR2; the triple mutant embryos secrete uniform cuticle with no denticle belts. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c in a wgl-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50CAR2; the triple mutant embryos have a wild-type cuticle pattern. The severity of the cuticle defects seen in embryos expressing AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4e22c is partially suppressed if they are also homozygous for RacGAP50CAR2; some aspects of the segmental pattern are rescued, with more naked cuticle separating the denticle belts. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c significantly rescues the cuticle pattern defects seen in RacGAP50CAR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50CAR2 embryos is unaffected if the embryos are also expressing nkdScer\UAS.cZa under the control of Scer\GAL4e22c.