FlyBase Allele Report: Dmel\tum[AR2]

General Information

Symbol

Dmel\tum^AR2

Species

D. melanogaster

Name

FlyBase ID

FBal0176033

Feature type

allele

Associated gene

Dmel\tum

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Jones and Bejsovec, 2005)

Mutagen

ethyl methanesulfonate

(Jones and Bejsovec, 2005)

Progenitor genotype

Cytology

Description

Amino acid replacement: ??term.

(Bejsovec, 2005.1)

Amino acid replacement: W470term.

(Jones and Bejsovec, 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,856,945..13,856,945 [-]

Nucleotide change:

G13856945A

Amino acid change:

W470term | tum-PA

Reported amino acid change:

W470term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Jones and Bejsovec, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage (with tum^DH15)

(Jones and Bejsovec, 2005, Zavortink et al., 2005)

lethal - all die during embryonic stage | recessive

(Jones and Bejsovec, 2005, Zavortink et al., 2005)

some die during embryonic stage (with tum^DH15)

(Jones and Bejsovec, 2005, Zavortink et al., 2005)

some die during embryonic stage | recessive

(Jones and Bejsovec, 2005, Zavortink et al., 2005)

Phenotype Manifest In

abdominal ventral denticle belt | embryonic stage

(Jones et al., 2010)

denticle

(Jones and Bejsovec, 2005)

denticle row

(Jones and Bejsovec, 2005)

embryonic/first instar larval cuticle

(Jones and Bejsovec, 2005)

embryonic epidermis & nucleus

(Jones and Bejsovec, 2005)

epithelium | embryonic stage

(Zavortink et al., 2005)

epithelium | embryonic stage (with Df(2R)Exel7128)

(Zavortink et al., 2005)

nucleus

(Zavortink et al., 2005)

nucleus (with Df(2R)Exel7128)

(Zavortink et al., 2005)

Detailed Description

Statement

Reference

tum^AR2 mutants show excess specification of naked cuticle when compared with the wild-type epidermal pattern.

(Jones et al., 2010)

Homozygous embryos have an excess of naked cuticle at the expense of denticles. The mutant embryos secrete an average of only 3.95 +/- 0.64 rows of denticles/belt in abdominal segments 3-6 (compared to the wild-type value of 5.95 +/- 0.15 rows of denticles/belt). Most denticles in the mutant belts are similar in size to the smaller denticles normally found in rows 1-4 of the wild-type belt pattern. Homozygous embryos accumulate large binucleate cells in the epidermis during stage 11.

(Jones and Bejsovec, 2005)

Binucleate cells are found in the embryonic epithelium of homozygous tum^AR2 and trans-heterozygous tum^AR2/Df(2R)Exel7128 embryos.

(Zavortink et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

tum^AR2 has lethal | recessive | embryonic stage phenotype, non-suppressible by Scer\GAL4^e22c/nkd^UAS.cZa

(Jones and Bejsovec, 2005)

Suppressor of

Statement

Reference

tum[+]/tum^AR2 is a suppressor of lethal | recessive | embryonic stage phenotype of arm⁸

(Jones et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

tum^AR2 has denticle phenotype, suppressible by Scer\GAL4^e22c/nkd^UAS.cZa

(Jones and Bejsovec, 2005)

tum^AR2 has embryonic/first instar larval cuticle phenotype, suppressible by Scer\GAL4^e22c/nkd^UAS.cZa

(Jones and Bejsovec, 2005)

NOT suppressed by

Statement

Reference

tum^AR2 has embryonic epidermis & nucleus phenotype, non-suppressible by Scer\GAL4^e22c/nkd^UAS.cZa

(Jones and Bejsovec, 2005)

Enhancer of

Statement

Reference

tum^AR2 is an enhancer of ventral denticle belt phenotype of nkd²

(Jones and Bejsovec, 2005)

tum^AR2 is an enhancer of embryonic/first instar larval cuticle phenotype of nkd²

(Jones and Bejsovec, 2005)

NOT Enhancer of

Statement

Reference

tum^AR2 is a non-enhancer of abdominal ventral denticle belt | embryonic stage phenotype of pav^B200

(Jones et al., 2010)

tum[+]/tum^AR2 is a non-enhancer of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, pan^ΔN.UAS

(Jones et al., 2010)

tum[+]/tum^AR2 is a non-enhancer of abdominal ventral denticle belt | embryonic stage phenotype of arm⁴

(Jones et al., 2010)

Suppressor of

Statement

Reference

tum[+]/tum^AR2 is a suppressor of abdominal ventral denticle belt | embryonic stage phenotype of arm⁸

(Jones et al., 2010)

tum^AR2 is a suppressor of embryonic/first instar larval cuticle phenotype of wg^PE2

(Jones and Bejsovec, 2005)

tum^AR2 is a suppressor of denticle phenotype of wg^l-12

(Jones and Bejsovec, 2005)

tum^AR2 is a suppressor of embryonic/first instar larval cuticle phenotype of wg^l-12

(Jones and Bejsovec, 2005)

tum^AR2 is a suppressor | partially of embryonic/first instar larval cuticle phenotype of Axn^UAS.GFP, Scer\GAL4^e22c

(Jones and Bejsovec, 2005)

tum^AR2 is a suppressor | partially of denticle | ectopic phenotype of Axn^UAS.GFP, Scer\GAL4^e22c

(Jones and Bejsovec, 2005)

NOT Suppressor of

Statement

Reference

tum^AR2 is a non-suppressor of abdominal ventral denticle belt | embryonic stage phenotype of pav^B200

(Jones et al., 2010)

tum[+]/tum^AR2 is a non-suppressor of abdominal ventral denticle belt | embryonic stage phenotype of Scer\GAL4^e22c, pan^ΔN.UAS

(Jones et al., 2010)

tum[+]/tum^AR2 is a non-suppressor of abdominal ventral denticle belt | embryonic stage phenotype of arm⁴

(Jones et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The phenotype of tum^AR2; pav^B200 double mutant embryos does not show significant differences from the tum^AR2 and pav^B200 single mutant phenotypes.

The elimination of naked cuticle observed in Scer\GAL4^e22c > pan^ΔN.Scer\UAS embryos is unchanged in a tum^AR2/+ mutant background.

The elimination of naked cuticle observed in arm⁴ mutant embryos is unchanged in a tum^AR2/+ mutant background.

Heterozygosity for tum^AR2 restores areas of naked cuticle between the belts to almost wide-type levels, and many more arm⁸ mutants are able to hatch into the first instar stage.

(Jones et al., 2010)

RacGAP50C^AR2 reduces the severity of the mutant phenotype seen in wg^l-12 embryos at the restrictive temperature; the double mutant embryos are larger than single mutant wg^l-12 embryos, and there is an increase in spacing between denticles in the double mutant embryos. Some naked cuticle is restored in wg^PE2 RacGAP50C^AR2 double mutant embryos. The excess naked cuticle phenotype seen in nkd² embryos is enhanced by RacGAP50C^AR2; the double mutant embryos have uniform naked cuticle with no denticle belts at all. The ability of wg^l-17/+ to suppress the excess naked cuticle phenotype of nkd² embryos is suppressed by RacGAP50C^AR2; the triple mutant embryos secrete uniform cuticle with no denticle belts. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c in a wg^l-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50C^AR2; the triple mutant embryos have a wild-type cuticle pattern. The severity of the cuticle defects seen in embryos expressing Axn^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^e22c is partially suppressed if they are also homozygous for RacGAP50C^AR2; some aspects of the segmental pattern are rescued, with more naked cuticle separating the denticle belts. Expression of nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c significantly rescues the cuticle pattern defects seen in RacGAP50C^AR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50C^AR2 embryos is unaffected if the embryos are also expressing nkd^Scer\UAS.cZa under the control of Scer\GAL4^e22c.