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General Information
Symbol
Dmel\tumAR2
Species
D. melanogaster
Name
FlyBase ID
FBal0176033
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G13856945A

Amino acid change:

W470term | tum-PA

Reported amino acid change:

W470term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: ??term.

Amino acid replacement: W470term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

tumAR2 mutants show excess specification of naked cuticle when compared with the wild-type epidermal pattern.

Homozygous embryos have an excess of naked cuticle at the expense of denticles. The mutant embryos secrete an average of only 3.95 +/- 0.64 rows of denticles/belt in abdominal segments 3-6 (compared to the wild-type value of 5.95 +/- 0.15 rows of denticles/belt). Most denticles in the mutant belts are similar in size to the smaller denticles normally found in rows 1-4 of the wild-type belt pattern. Homozygous embryos accumulate large binucleate cells in the epidermis during stage 11.

Binucleate cells are found in the embryonic epithelium of homozygous tumAR2 and trans-heterozygous tumAR2/Df(2R)Exel7128 embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
Suppressor of
Statement
Reference

tum[+]/tumAR2 is a suppressor | partially of lethal | recessive | embryonic stage phenotype of arm8

Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference

tumAR2 has embryonic epidermis & nucleus phenotype, non-suppressible by Scer\GAL4e22c/nkdUAS.cZa

Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The phenotype of tumAR2; pavB200 double mutant embryos does not show significant differences from the tumAR2 and pavB200 single mutant phenotypes.

The elimination of naked cuticle observed in Scer\GAL4e22c > panΔN.Scer\UAS embryos is unchanged in a tumAR2/+ mutant background.

The elimination of naked cuticle observed in arm4 mutant embryos is unchanged in a tumAR2/+ mutant background.

Heterozygosity for tumAR2 restores areas of naked cuticle between the belts to almost wide-type levels, and many more arm8 mutants are able to hatch into the first instar stage.

RacGAP50CAR2 reduces the severity of the mutant phenotype seen in wgl-12 embryos at the restrictive temperature; the double mutant embryos are larger than single mutant wgl-12 embryos, and there is an increase in spacing between denticles in the double mutant embryos. Some naked cuticle is restored in wgPE2 RacGAP50CAR2 double mutant embryos. The excess naked cuticle phenotype seen in nkd2 embryos is enhanced by RacGAP50CAR2; the double mutant embryos have uniform naked cuticle with no denticle belts at all. The ability of wgl-17/+ to suppress the excess naked cuticle phenotype of nkd2 embryos is suppressed by RacGAP50CAR2; the triple mutant embryos secrete uniform cuticle with no denticle belts. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c in a wgl-17/+ background results in fusions between denticle belts due to loss of intervening naked cuticle. This effect is suppressed if the embryos are also heterozygous for RacGAP50CAR2; the triple mutant embryos have a wild-type cuticle pattern. The severity of the cuticle defects seen in embryos expressing AxnScer\UAS.T:Avic\GFP under the control of Scer\GAL4e22c is partially suppressed if they are also homozygous for RacGAP50CAR2; some aspects of the segmental pattern are rescued, with more naked cuticle separating the denticle belts. Expression of nkdScer\UAS.cZa under the control of Scer\GAL4e22c significantly rescues the cuticle pattern defects seen in RacGAP50CAR2 embryos, although embryonic lethality is not rescued. However, the accumulation of binucleate cells in the epidermis that is seen during stage 11 in RacGAP50CAR2 embryos is unaffected if the embryos are also expressing nkdScer\UAS.cZa under the control of Scer\GAL4e22c.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments

Expression of tumScer\UAS.cSa driven by Scer\GAL4e22c rescues the epidermal pattern defects of tumAR2 mutants.

Expression of tumΔNLS.Scer\UAS driven by Scer\GAL4e22c fails to rescue the epidermal pattern defects of tumAR2 mutants.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Induced on: a wgl-12 chromosome. Selected as: a modifier of the wgl-12 mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)