FlyBase Allele Report: Dmel\fru[F]

General Information

Symbol

Dmel\fru^F

Species

D. melanogaster

Name

FlyBase ID

FBal0191136

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

TI{TI}fru^F

Carried in Construct

Key Links

Allele class

Mutagen

FLPase

Nature of the Allele

Allele class

Mutagen

FLPase

(Demir and Dickson, 2005)

Progenitor genotype

fru^F-5'half

(Demir and Dickson, 2005)

fru^X-3'half

(Demir and Dickson, 2005)

Associated Insertion(s)

TI{TI}fru^F

Cytology

Description

FLP-mediated recombination between fru^F-5'half and fru^X-3'half has produced a fru gene in which the male specific splice donor site of the S-exon has been mutated (GTAAGC -> CTGACC) to prevent male specific splicing of fru transcripts. Coding potential of the S exon is unaffected. No marker genes or vector sequence remain apart from an FRT site about 0.6 Kb 3' of the mutated S-exon.

(Demir and Dickson, 2005)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage

(Zhang et al., 2020)

abnormal chemosensory behavior | adult stage (with fru^ΔB)

(Zhang et al., 2020)

abnormal chemosensory behavior | adult stage (with fru^ΔC)

(Zhang et al., 2020)

abnormal courtship behavior | male (with fru^4-40)

(Demir and Dickson, 2005)

abnormal mating behavior (with fru^B1)

(von Philipsborn et al., 2014)

abnormal mating behavior (with fru^B2)

(von Philipsborn et al., 2014)

abnormal mating behavior (with fru^C1)

(von Philipsborn et al., 2014)

abnormal mating behavior (with fru^C2)

(von Philipsborn et al., 2014)

abnormal mating behavior (with fru^C3)

(von Philipsborn et al., 2014)

abnormal mating behavior (with fru^ΔAmyc)

(von Philipsborn et al., 2014)

abnormal neuroanatomy (with fru^4-40)

(Castellanos et al., 2013)

abnormal neuroanatomy | adult stage | male

(von Philipsborn et al., 2014)

abnormal neuroanatomy | adult stage | male (with fru^B2)

(von Philipsborn et al., 2014)

abnormal neuroanatomy | adult stage | male (with fru^C1)

(von Philipsborn et al., 2014)

abnormal neurophysiology | adult stage

(Zhang et al., 2020)

abnormal neurophysiology | adult stage (with fru^ΔB)

(Zhang et al., 2020)

abnormal neurophysiology | adult stage (with fru^ΔC)

(Zhang et al., 2020)

abnormal neurophysiology | adult stage | conditional

(Sethi et al., 2019)

abnormal neurophysiology | adult stage | semidominant | conditional

(Sethi et al., 2019)

abnormal song (with fru^B2)

(von Philipsborn et al., 2014)

abnormal song (with fru^C1)

(von Philipsborn et al., 2014)

abnormal song (with fru^ΔAmyc)

(von Philipsborn et al., 2014)

female fertile (with fru^4-40)

(Demir and Dickson, 2005)

viable (with fru^B2)

(von Philipsborn et al., 2014)

viable (with fru^C1)

(von Philipsborn et al., 2014)

viable (with fru^ΔAmyc)

(von Philipsborn et al., 2014)

Phenotype Manifest In

adult fruitless aDT-b (female) neuron | ectopic | male

(von Philipsborn et al., 2014)

adult fruitless aDT-b (male) neuron

(von Philipsborn et al., 2014)

adult fruitless aDT-b (male) neuron (with fru^B2)

(von Philipsborn et al., 2014)

adult fruitless aDT-b (male) neuron (with fru^C1)

(von Philipsborn et al., 2014)

adult fruitless aSP4 (male) neuron | male

(von Philipsborn et al., 2014)

adult fruitless aSP4 (male) neuron | male (with fru^C1)

(von Philipsborn et al., 2014)

adult fruitless aSP-a (male) neuron

(von Philipsborn et al., 2014)

adult fruitless aSP-b (male) neuron

(von Philipsborn et al., 2014)

adult fruitless dPR1 neuron

(von Philipsborn et al., 2014)

adult fruitless pIP10 (male) neuron

(von Philipsborn et al., 2014)

adult fruitless vAB3 (female) neuron | ectopic | male

(von Philipsborn et al., 2014)

adult fruitless vAB3 (male) neuron

(von Philipsborn et al., 2014)

adult fruitless vAB3 (male) neuron (with fru^C1)

(von Philipsborn et al., 2014)

adult fruitless vPR1 neuron

(von Philipsborn et al., 2014)

adult fruitless vPR6 (male) neuron

(von Philipsborn et al., 2014)

adult fruitless vPR6 (male) neuron (with fru^C1)

(von Philipsborn et al., 2014)

adult olfactory receptor neuron Ir84a

(Zhang et al., 2020)

adult olfactory receptor neuron Or47b

(Zhang et al., 2020)

adult olfactory receptor neuron Or47b (with fru^ΔB)

(Zhang et al., 2020)

adult olfactory receptor neuron Or47b (with fru^ΔC)

(Zhang et al., 2020)

adult olfactory receptor neuron Or47b | adult stage | conditional

(Sethi et al., 2019)

muscle cell of muscle of Lawrence (with fru^4-40)

(Demir and Dickson, 2005)

serotonergic abdominal giant (male) neuron (with fru^4-40)

(Castellanos et al., 2013)

Detailed Description

Statement

Reference

Under control conditions, fru^F heterozygotes and fru^F/fru^4-40 transheterozygotes do not show significant changes in mitotic index in the female and male adult posterior midgut, as compared to controls.

(Hudry et al., 2016)

fru^{ΔA.T:Hsap\MYC}/fru^F, fru^B1/fru^F, fru^B2/fru^F, fru^C1/fru^F, fru^C2/fru^F and fru^C3/fru^F males show significantly reduced copulation success compared to controls in single-pair courtship assays.

fru^B2/fru^F males show courtship song defects: the interpulse interval of the pulse song is longer and more varied than normal. Sine song and the carrier frequency of pulse song are normal.

fru^C1/fru^F males show courtship song defects: sine song is almost completely absent, the interpulse interval of the pulse song is longer and more varied than normal, the carrier frequency of pulse song is highly variable and in the dark, pulse song is dramatically reduced.

fru^{ΔA.T:Hsap\MYC}/fru^F males show courtship song defects: the interpulse interval of the pulse song is longer and more varied than normal, the carrier frequency of sine song is increased and the carrier frequency of pulse song is reduced.

fru^F males show a significant decrease in the number of pIP10, dPR1, vPR1, vPR6, aDT2/mAL, aSP1 and aSP2 neuronal cells per hemisphere compared to fru[+] males. The aDT2, aSP4 and vAB3 neurons show an apparently complete transformation to female-like morphology in these males.

fru^{ΔA.T:Hsap\MYC}/fru^F males show no significant change in the number of pMP4/P1, pIP10, dPR1, vPR1, vPR6, aDT2/mAL, aSP1 and aSP2 neuronal cells per hemisphere compared to fru[+] males.

fru^B2/fru^F males show a significant decrease in the number of aDT2/mAL neuronal cells per hemisphere compared to fru[+] males, but no significant change in the number of pMP4/P1, pIP10, dPR1, vPR1, vPR6, aSP1 or aSP2 neuronal cells per hemisphere.

fru^C1/fru^F males show a significant decrease in the number of vPR6 and aDT2/mAL neuronal cells per hemisphere compared to fru[+] males, but no significant change in the number of pMP4/P1, pIP10, dPR1, vPR1, aSP1 or aSP2 neuronal cells per hemisphere. The aDT2, aSP4 and vAB3 neurons show female-like arborisations in these males.

(von Philipsborn et al., 2014)

In fru^F/fru^4-40 males the post-embryonic Ilp7 neurons in the posterior adult ventral nerve cord are feminised; the normally female-specific ventral subset of 3-4 post-embryonic Ilp7 neurons is present adjacent to the usual embryonic Ilp7 neurons. The dorsal cluster of post-embryonic Ilp7 neurons seen in both males and females is retained but, as in females, no longer expresses serotonin.

(Castellanos et al., 2013)

The male specific 'muscle of lawrence' is absent in fru^F/fru^4-40 males. fru^F/fru^4-40 males fail to court females. This does not change in the presence of wild-type males, whom they fail to compete with in competition for copulation with a wild-type virgin female. fru³/fru^4-40 males show a small but significant increase in courtship of other males compared to wild-type. Unlike wild-type males these mutant males form male mating chains.

(Demir and Dickson, 2005)

The sexual dimorphism of the volumes of the DA1, VA1v and VL2a antennal glomeruli is eliminated in fru^F mutant animals.

(Stockinger et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

fru[+]/fru^F is a non-enhancer of abnormal courtship behavior | male phenotype of Scer\GAL4^elav.PLu, tra^UAS.cFa

(Demir and Dickson, 2005)

NOT Suppressor of

Statement

Reference

fru[+]/fru^F is a non-suppressor of abnormal courtship behavior | male phenotype of Scer\GAL4^elav.PLu, tra^UAS.cFa

(Demir and Dickson, 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduction in courtship behaviour seen in tra^Scer\UAS.cFa; Scer\GAL4^elav.PLu males is unaffected by fru^F/+.

(Demir and Dickson, 2005)

Xenogenetic Interactions

Statement

Reference

Photoactivation of fru-expressing neurons in headless males expressing Rnor\P2rx2^Scer\UAS.cLa under the control of Scer\GAL4^fru-GAL4 in a fru^F/fru^GAL4 background produces courtship songs with sine and pulse songs, but the acoustic characteristics of these songs show broad bimodal distributions, with peaks near the typical male and female modes, suggesting that these animals represent a mixed population.

(Clyne and Miesenbock, 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66873

TI{TI}fru^F/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fru^F

(Bonheur et al., 2023, Ishii et al., 2020, Wohl et al., 2020, Zhang et al., 2020, Sethi et al., 2019, Garner et al., 2018, Jang et al., 2017, Hudry et al., 2016, von Philipsborn et al., 2014, Kohl et al., 2013, Rideout et al., 2010, Dankert et al., 2009, Clyne and Miesenbock, 2008)

fruF

(Castellanos et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (17)

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