FlyBase Allele Report: Dmel\mir-9a[E39]

General Information

Symbol

Dmel\mir-9a^E39

Species

D. melanogaster

Name

FlyBase ID

FBal0198147

Feature type

allele

Associated gene

Dmel\mir-9a

Associated Insertion(s)

TI{TI}mir-9a^E39

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Li et al., 2006)

I-SceI

(Li et al., 2006)

FLPase

(Li et al., 2006)

ends-out gene targeting

(Li et al., 2006)

Progenitor genotype

Associated Insertion(s)

TI{TI}mir-9a^E39

Cytology

Description

The 78bp mir-9a precursor DNA sequence has been replaced by a w[+] marker.

(Li et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size (with mir-9a^J22)

(Suh et al., 2015)

fertile

(Li et al., 2006)

increased cell death | somatic clone

(Bejarano et al., 2010)

increased cell death | third instar larval stage (with mir-9a^J22)

(Bejarano et al., 2010)

viable

(Li et al., 2006)

visible

(Bejarano et al., 2010)

visible (with mir-9a^J22)

(Suh et al., 2015, Bejarano et al., 2010)

visible | somatic clone

(Bejarano et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

mir-9a^E39/mir-9a^J22 mutant flies have a reduced body length, wing length and pupal volume. Haemolymph glucose and trehalose levels are also reduced.

(Suh et al., 2015)

Homozygotes and mir-9a^J22/mir-9a^E39 animals lack approximately 50% of the posterior wing margin in all individuals and approximately 10% of animals also show mild loss of anterior wing margin.

Abundant cell death is seen in the wing pouch region of the wing disc in mir-9a^J22/mir-9a^E39 third instar larvae, in contrast to wild-type controls. There is substantially more cell death in the dorsal compartment than in the ventral compartment. Cell death is not seen in the pronotum region of the disc in the mutant larvae.

mir-9a^J22/mir-9a^E39 but not homozygous adults show a mild increase in notum microchaete density compared to controls.

mir-9a^J22/mir-9a^E39 adults often have ectopic dorsocentral and anterior postalar bristles.

Wings carrying large homozygous clones show notching along the posterior wing margin.

Small homozygous clones in the wing disc contain apoptotic cells.

(Bejarano et al., 2010)

37% of mir-9a^J22 third instar larvae have an extra ddaE or ddaF neuron in one or two but not other hemisegments.

(Li et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has decreased body size phenotype, suppressible | partially by sNPF-R[+]/sNPF-R^MI00427

(Suh et al., 2015)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible | partially by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/Diap1^UAS.cHa

(Bejarano et al., 2010)

Enhancer of

Statement

Reference

mir-9a^E39/mir-9a[+] is an enhancer of visible | adult stage phenotype of TBPH^Q367X

(Li et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/Diap1^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing disc phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible | partially by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has dorsocentral bristle phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^UAS.cHa

(Bejarano et al., 2010)

NOT suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has wing pouch phenotype, non-suppressible by sens^E2/sens[+]

(Bejarano et al., 2010)

Enhancer of

Statement

Reference

mir-9a^E39/mir-9a[+] is an enhancer of scutellar bristle | increased number phenotype of TBPH^Q367X

(Li et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of sNPF-R^MI00427 slightly suppresses the reduction in body length seen in mir-9a^E39/mir-9a^J22 mutant flies.

(Suh et al., 2015)

mir-9a^E39/+ significantly enhances the supernumerary scutellar bristle phenotype of TBPH^Q367X/+ flies.

(Li et al., 2013)

Expression of th^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 completely rescues the wing notching phenotype of mir-9a^J22/mir-9a^E39 animals.

sens^E2/+ results in an approximately 35% reduction in the loss of wing margin caused by mir-9a^J22/mir-9a^E39. sens^E2/+ does not rescue the increased cell death seen in the wing discs of mir-9a^J22/mir-9a^E39 animals.

Bx^hdpR26/+ fully rescues the adult wing margin defects of mir-9a^J22 homozygotes and mir-9a^J22/mir-9a^E39 animals. Bx^hdpR26/+ strongly suppresses the ectopic apoptosis seen in the wing discs of mir-9a^J22/mir-9a^E39 animals.

The presence of Bx^hdpR26/+ partially suppresses the formation of ectopic anterior postalar bristles seen in mir-9a^J22/mir-9a^E39 adults, but has only mild effects on the formation of ectopic dorsocentral bristles seen in these flies.

sens^E2/+ partially suppresses the formation of ectopic dorsocentral and anterior postalar bristles seen in mir-9a^J22/mir-9a^E39 adults.

(Bejarano et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 completely rescues the wing notching phenotype of mir-9a^J22/mir-9a^E39 animals.

(Bejarano et al., 2010)

Complementation and Rescue Data

Rescued by

mir-9a^J22/mir-9a^E39 is rescued by mir-9a^{UAS.cSa.DsRed2}/Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

mir-9a^J22/mir-9a^E39 is rescued by Scer\GAL4^ap-md544/mir-9a^UAS.cLa

(Bejarano et al., 2010)

Comments

Expression of mir-9a^{Scer\UAS.T:Disc\RFP-DsRed2.cSa} under the control of Scer\GAL4^Ilp2.PR rescues the reduction in body and wing length seen in mir-9a^E39/mir-9a^J22 mutant flies.

(Suh et al., 2015)

Expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 completely rescues the continuity of the wing margin in mir-9a^J22/mir-9a^E39 animals. In the developing wing disc, the increased apoptosis seen in the wing pouch in mir-9a^J22/mir-9a^E39 animals is rescued in the dorsal compartment by expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544.

(Bejarano et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E39

(Li et al., 2006)

miR-9a^E39

(Suh et al., 2015, Cassidy et al., 2013, Li et al., 2013)

mir-9a^E39

(Cassidy et al., 2019, Bejarano et al., 2010, Loya et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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