FlyBase Allele Report: Dmel\mir-9a[J22]

mir-9a^E39/mir-9a^J22 mutant flies have a reduced body length, wing length and pupal volume. Haemolymph glucose and trehalose levels are also reduced.

(Suh et al., 2015)

Homozygous and mir-9a^J22/Df(3L)ED228 males show a significant decrease in median lifespan compared to controls.

Homozygous adults show significant defects in the hemolymph-brain barrier (as determined using a dye injection assay).

(Chen et al., 2014)

Homozygotes and mir-9a^J22/mir-9a^E39 animals lack approximately 50% of the posterior wing margin in all individuals and approximately 10% of animals also show mild loss of anterior wing margin.

Abundant cell death is seen in the wing pouch region of the wing disc in mir-9a^J22/mir-9a^E39 third instar larvae, in contrast to wild-type controls. Cell death is not seen in the pronotum region of the disc in the mutant larvae.

Homozygous and mir-9a^J22/mir-9a^E39 adults show a mild increase in notum microchaete density compared to controls.

mir-9a^J22/mir-9a^E39 adults often have ectopic dorsocentral and anterior postalar bristles.

(Bejarano et al., 2010)

mir-9a^J22 homozygous flies lack anterior and posterior wing margins.

Heterozygotes show normal wing development.

(Biryukova et al., 2009)

Homozygous mir-9a^J22 mutants exhibit posterior wing margin defects. No defects are seen in heterozygous mir-9a^J22 flies.

A mir-9a^J22/+ background enhances the wing margin defects seen when mir-9a^{sponge.Scer\UAS.T:Avic\GFP-EGFP} is expressed under the control of Scer\GAL4^αTub84B.PL.

(Loya et al., 2009)

29% of mir-9a^J22 third instar larvae have an extra ddaE or ddaF neuron in one or two but not other hemisegments.

Mutant adults lack the posterior wing margin (100% penetrance), while the veins are normal in appearance. The anterior wing margin contains ectopic sensory bristles in about 40% of mutant flies.

14.3% of mutant flies have 3-4 scutellar bristles per heminotum (instead of the wild-type number of 2 per heminotum). These extra bristles have normal shaft and socket cells and are accompanied by a sensory neuron.

Mutant third instar wing discs have a high frequency of ectopic sensory organ precursors (SOPs) in the notum region.

(Li et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has decreased body size phenotype, suppressible | partially by sNPF-R[+]/sNPF-R^MI00427

(Suh et al., 2015)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/Diap1^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible | partially by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22 has visible phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22 has increased cell death phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22 has visible phenotype, suppressible by Bx^hdp-n60

(Biryukova et al., 2009)

mir-9a^J22 has visible phenotype, suppressible | partially by sens^E58/sens[+]

(Li et al., 2006)

NOT suppressed by

Statement

Reference

mir-9a^J22 has increased cell death phenotype, non-suppressible by sens^E2/sens[+]

(Bejarano et al., 2010)

Enhancer of

Statement

Reference

mir-9a^J22/mir-9a[+] is an enhancer of visible | adult stage phenotype of TBPH^Q367X

(Li et al., 2013)

mir-9a^J22/mir-9a[+] is an enhancer of visible phenotype of Bx^D124

(Biryukova et al., 2009)

mir-9a^J22/mir-9a[+] is an enhancer of visible phenotype of Bx^D128

(Biryukova et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has wing disc phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible | partially by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has dorsocentral bristle phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/Diap1^UAS.cHa

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible | partially by sens^E2/sens[+]

(Bejarano et al., 2010)

mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22 has wing margin phenotype, suppressible by Bx[+]/Bx^hdpR26

(Bejarano et al., 2010)

mir-9a^J22 has wing margin phenotype, suppressible by Bx^hdp-n60

(Biryukova et al., 2009)

mir-9a^J22 has wing margin phenotype, suppressible | partially by sens^E58/sens[+]

(Li et al., 2006)

NOT suppressed by

Statement

Reference

mir-9a^J22/mir-9a^E39 has wing pouch phenotype, non-suppressible by sens^E2/sens[+]

(Bejarano et al., 2010)

Enhancer of

Statement

Reference

mir-9a^J22/mir-9a[+] is an enhancer of scutellar bristle | increased number phenotype of TBPH^Q367X

(Li et al., 2013)

mir-9a^J22/mir-9a[+] is an enhancer of wing margin phenotype of Bx^D124

(Biryukova et al., 2009)

mir-9a^J22/mir-9a[+] is an enhancer of wing margin phenotype of Bx^D128

(Biryukova et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of sNPF-R^MI00427 slightly suppresses the reduction in body length seen in mir-9a^E39/mir-9a^J22 mutant flies.

(Suh et al., 2015)

mir-9a^J22/+ significantly enhances the supernumerary scutellar bristle phenotype of TBPH^Q367X/+ flies.

(Li et al., 2013)

Expression of th^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 completely rescues the wing notching phenotype of mir-9a^J22/mir-9a^E39 animals.

sens^E2/+ results in an approximately 35% reduction in the loss of wing margin caused by mir-9a^J22/mir-9a^E39. sens^E2/+ does not rescue the increased cell death seen in the wing discs of mir-9a^J22/mir-9a^E39 animals.

Bx^hdpR26/+ fully rescues the adult wing margin defects of mir-9a^J22 homozygotes and mir-9a^J22/mir-9a^E39 animals. Bx^hdpR26/+ strongly suppresses the ectopic apoptosis seen in the wing discs of mir-9a^J22/mir-9a^E39 animals.

The presence of Bx^hdpR26/+ partially suppresses the formation of ectopic anterior postalar bristles seen in mir-9a^J22/mir-9a^E39 adults, but has only mild effects on the formation of ectopic dorsocentral bristles seen in these flies.

sens^E2/+ partially suppresses the formation of ectopic dorsocentral and anterior postalar bristles seen in mir-9a^J22/mir-9a^E39 adults.

(Bejarano et al., 2010)

The loss of wing margin seen in mir-9a^J22 flies is completely suppressed by Bx^hdp-n60.

The wing margin phenotype seen in Bx^D124/Y and Bx^D128/Y flies is enhanced by mir-9a^J22/+.

(Biryukova et al., 2009)

One copy of sens^E58 substantially rescues the wing margin defects of mir-9a^J22 homozygotes; about 30% of the double mutant flies have wings that are indistinguishable from wild type, while others lack only a small part of the posterior margin. The bristles on the anterior wing margin resemble wild type in the double mutant flies.

(Li et al., 2006)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^ap-md544 completely rescues the wing notching phenotype of mir-9a^J22/mir-9a^E39 animals.

(Bejarano et al., 2010)

Complementation and Rescue Data

Rescued by

mir-9a^J22/mir-9a^E39 is rescued by mir-9a^{UAS.cSa.DsRed2}/Scer\GAL4^Ilp2.PR

(Suh et al., 2015)

mir-9a^J22/mir-9a^E39 is rescued by Scer\GAL4^ap-md544/mir-9a^UAS.cLa

(Bejarano et al., 2010)

Partially rescued by

mir-9a^J22 is partially rescued by mir-9a^UAS.cLa/Scer\GAL4^vg.PU

(Li et al., 2006)

Comments

Expression of mir-9a^{Scer\UAS.T:Disc\RFP-DsRed2.cSa} under the control of Scer\GAL4^Ilp2.PR rescues the reduction in body and wing length seen in mir-9a^E39/mir-9a^J22 mutant flies.

(Suh et al., 2015)

Expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 completely rescues the continuity of the wing margin in mir-9a^J22/mir-9a^E39 animals. In the developing wing disc, the increased apoptosis seen in the wing pouch in mir-9a^J22/mir-9a^E39 animals is rescued in the dorsal compartment by expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544.

(Bejarano et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

J22

(Li et al., 2006)

miR-9a^J22

(Cassidy et al., 2013, Li et al., 2013, Loya et al., 2009)

mir-9a^J22

(Cassidy et al., 2019, Bejarano et al., 2010, Biryukova et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (9)

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