FlyBase Allele Report: Dmel\Drp1[GD10456]

General Information

Symbol

Dmel\Drp1^GD10456

Species

D. melanogaster

Name

FlyBase ID

FBal0206904

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

Carried in Construct

P{GD10456}

Also Known As

DRP1 RNAi, UAS-Drp1 RNAi, UAS-Drp1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10456}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10456

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Duboff et al., 2012)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased rate of movement | adult stage, with Scer\GAL4^sca.PU

(Dubal et al., 2022)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Drp1^GD10456 under the control of Scer\GAL4^dome-PG14 leads to the blood progenitors in the third instar larva lymph gland to show mitochondria with longer branches; there is a decrease in the number of crystal cells.

(Ray et al., 2021)

Loss of Drp1, through expression of Drp1^GD10456 under the control of Scer\GAL4^fog results in defects in the ventral furrow, with a quarter of embryos exhibiting an irregular shallow line in place of the ventral furrow. twi-positive cells are seen at the surface in the region of the ventral furrow, indicating asynchronous and/or delayed invagination of the blastoderm. More commonly, the T-bar fails to forma 'ring' as it does in wild-type embryos.

(Ratnaparkhi, 2013)

Expression of Drp1^GD10456 under the control of Scer\GAL4^elav.PU has no effect on mitochondrial length in adult neurons. The amount of cell death and cell cycle activation are similar to controls, as are levels of superoxide production.

(Duboff et al., 2012)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10456

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Drp1^GD10456, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Drp1^GD10456, Scer\GAL4^elav.PU is an enhancer of abnormal neuroanatomy phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^GD10456, Scer\GAL4^elav.PU is an enhancer of abnormal cell death phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^GD10456, Scer\GAL4^elav.PU is an enhancer of abnormal cell cycle phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Suppressor of

Statement

Reference

Drp1^GD10456, Scer\GAL4^NP5130 is a suppressor of increased cell death phenotype of Hsc70-5^KK100233, Scer\GAL4^NP5130

(Banerjee and Chinthapalli, 2014)

Drp1^GD10456, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

Drp1^GD10456, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Drp1^GD10456, Scer\GAL4^elav.PU is an enhancer of adult brain phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Drp1^GD10456, Scer\GAL4^elav.PU is an enhancer of mitochondrion phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav.PU

(Duboff et al., 2012)

Suppressor of

Statement

Reference

Drp1^GD10456, Scer\GAL4^Tub.PU is a suppressor of mitochondrion phenotype of Hsc70-5^KK100233, Scer\GAL4^Tub.PU

(Banerjee and Chinthapalli, 2014)

Drp1^GD10456, Scer\GAL4^NP5130 is a suppressor of adult midgut progenitor cell phenotype of Hsc70-5^KK100233, Scer\GAL4^NP5130

(Banerjee and Chinthapalli, 2014)

Drp1^GD10456, Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Drp1^GD10456, Scer\GAL4^Bx-MS1096 is a suppressor | partially of wing | male limited phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Drp1^GD10456, Scer\GAL4^Mhc.PU is a suppressor of mitochondrion phenotype of Scer\GAL4^Mhc.PU, fog^UAS.cRa

(Ratnaparkhi, 2013)

Drp1^GD10456, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pink1^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Poole et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Drp1^GD10456 results in a 77-83% suppression of the wing phenotype found in male flies expressing fog^Scer\UAS.cRa under the control of Scer\GAL4^Bx-MS1096, suggesting that loss of mitochondrial fission inhibits fog signalling.

Expression of Drp1^GD10456 suppresses the mitochondrial defects found upon expression of fog^Scer\UAS.cRa under the control of Scer\GAL4^Mhc.PU.

(Ratnaparkhi, 2013)

The rough eye phenotype of flies expressing Pink1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of Drp1^GD10456.

(Poole et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of Drp1^GD10456 enhances the increase in mitochondria length seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU. Increased levels of neurodegeneration and cell cycle activation are also seen.

Expression of Drp1^GD10456 enhances the increase in superoxide production seen when Hsap\MAPT^{R406W.Scer\UAS} is expressed under the control of Scer\GAL4^elav.PU.

(Duboff et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v44155

w¹¹¹⁸; P{GD10456}v44155/TM3

v44156

w¹¹¹⁸; P{GD10456}v44156/TM3