FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\KhcGD12278
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General Information
Symbol
Dmel\KhcGD12278
Species
D. melanogaster
Name
FlyBase ID
FBal0207442
Feature type
allele
Associated gene
Dmel\Khc
Associated Insertion(s)
Carried in Construct
P{GD12278}
Also Known As
KHC RNAi
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD12278}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD12278
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  amyotrophic lateral sclerosis type 10
      modeled by Hsap\TARDBPUAS.cIb
      (Ueda et al., 2024)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal body color, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      abnormal locomotor behavior | adult stage, with Scer\GAL4Gli-J29-Gal4

      (Schmidt et al., 2012)

      abnormal locomotor behavior | adult stage, with Scer\GAL4moody.SPG

      (Schmidt et al., 2012)

      abnormal locomotor behavior | adult stage, with Scer\GAL4Ntan1-Mz97

      (Schmidt et al., 2012)

      abnormal locomotor behavior | adult stage, with Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      abnormal locomotor behavior | larval stage, with Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      abnormal neuroanatomy, with Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4moody.SPG

      (Schmidt et al., 2012)

      abnormal neuroanatomy | third instar larval stage, with Scer\GAL4nrv2.PS

      (Schmidt et al., 2012)

      abnormal neurophysiology | third instar larval stage, with Scer\GAL4Gli-J29-Gal4

      (Schmidt et al., 2012)

      abnormal neurophysiology | third instar larval stage, with Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      lethal, with Scer\GAL4elav-C155, Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      lethal - all die during P-stage, with Scer\GAL4Mef2.PR

      (Schnorrer et al., 2010)

      majority die during pupal stage, with Scer\GAL4VGlut1-OK371

      (Zhu et al., 2013)

      majority die during third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Zhu et al., 2013)

      partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu

      (Neely et al., 2010)

      partially lethal - majority die, with Khc8, Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      partially lethal - majority die, with Scer\GAL4elav-C155

      (Schmidt et al., 2012)

      partially lethal - majority die, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      partially lethal - majority die, with Scer\GAL4repo.PL, Scer\GAL4repo

      (Schmidt et al., 2012)

      some die during pupal stage, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)

      viable, with Scer\GAL4tin.CΔ4

      (Neely et al., 2010)

      visible

      (Talmat-Amar et al., 2011)

      visible, with Scer\GAL4pnr-MD237

      (Mummery-Widmer et al., 2009)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Animals expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show reduced viability, the severity of which is increased if the flies are also heterozygous for Khc8.

      Animals expressing KhcGD12278 under the control of Scer\GAL4elav-C155 show reduced viability.

      Animals expressing KhcGD12278 under the simultaneous control of Scer\GAL4elav-C155, Scer\GAL4repo.PL and Scer\GAL4repo show complete lethality.

      Adults expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show a highly penetrant spastic locomotion defect.

      Larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show an abnormal pattern of locomotion.

      The peripheral nerves often appear swollen in animals expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo. The nerves contain bulges, with the number of bulges in general correlating with the length of the nerve. Mitochondria are missing over large stretches of the glia of the peripheral nervous system and accumulate in the perinuclear domain. In addition, the formation of the mitochondrial mesh that normally forms throughout the entire glial cell is completely disrupted.

      Adults expressing KhcGD12278 under the control of either Scer\GAL4moody.SPG, Scer\GAL4Gli-J29-Gal4 or Scer\GAL4Mz97 show locomotion defects, but do not show a spastic phenotype.

      Wrapping glial cells do not engulf the entire length of the axonal fascicle in the peripheral nerves of third instar larvae expressing KhcGD12278 under the control of Scer\GAL4nrv2.PS, but no nerve bulges are seen.

      Peripheral nerves of third instar larvae expressing KhcGD12278 under the control of Scer\GAL4moody.SPG show bulges.

      The amplitude of the evoked excitatory junction potentials (EJP) (measured after activating the A6 abdominal nerve) is dramatically reduced compared to wild type in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4Gli-J29-Gal4. In addition, the response of the muscle is delayed. The amplitude of spontaneous miniature EJPs is normal in these animals.

      The amplitude of the evoked excitatory junction potentials (EJP) (measured after activating the A6 abdominal nerve) is dramatically reduced compared to wild type in third instar larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo. In addition, the response of the muscle is delayed. The amplitude of spontaneous miniature EJPs is normal in these animals.

      The amplitudes of spontaneous and evoked EJPs (measured after activating the A6 abdominal nerve) are not significantly different to wild-type in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4nrv2.PS.

      (Schmidt et al., 2012)

      Expression of KhcGD12278 in adult motoneurons of flies under the control of Scer\GAL4RapGAP1-OK6 lead to the development of unexpanded wings.

      (Talmat-Amar et al., 2011)

      Adults expressing KhcGD12278 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.

      Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 80-90% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

      Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of KhcGD12278 enhances the multiple wing hair phenotype caused by expression of kermitScer\UAS.cLa under the control of Scer\GAL4Bx-MS1096.

      (Lin and Katanaev, 2013)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of Dana\KhcRNAi-res.Scer\UAS rescues the locomotor defects seen in adults and larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo.

      Co-expression of Dana\KhcRNAi-res.Scer\UAS rescues the reduced excitatory junction potential amplitude seen in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4Gli-J29-Gal4.

      (Schmidt et al., 2012)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      KhcGD12278
      khcdsRNA
      (Schmidt et al., 2012)
      Name Synonyms
      Secondary FlyBase IDs
        References (17)