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General Information
Symbol
Dmel\KhcGD12278
Species
D. melanogaster
Name
FlyBase ID
FBal0207442
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
KHC RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Animals expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show reduced viability, the severity of which is increased if the flies are also heterozygous for Khc8.

Animals expressing KhcGD12278 under the control of Scer\GAL4elav-C155 show reduced viability.

Animals expressing KhcGD12278 under the simultaneous control of Scer\GAL4elav-C155, Scer\GAL4repo.PL and Scer\GAL4repo show complete lethality.

Adults expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show a highly penetrant spastic locomotion defect.

Larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo show an abnormal pattern of locomotion.

The peripheral nerves often appear swollen in animals expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo. The nerves contain bulges, with the number of bulges in general correlating with the length of the nerve. Mitochondria are missing over large stretches of the glia of the peripheral nervous system and accumulate in the perinuclear domain. In addition, the formation of the mitochondrial mesh that normally forms throughout the entire glial cell is completely disrupted.

Adults expressing KhcGD12278 under the control of either Scer\GAL4moody.SPG, Scer\GAL4Gli-J29-Gal4 or Scer\GAL4Mz97 show locomotion defects, but do not show a spastic phenotype.

Wrapping glial cells do not engulf the entire length of the axonal fascicle in the peripheral nerves of third instar larvae expressing KhcGD12278 under the control of Scer\GAL4nrv2.PS, but no nerve bulges are seen.

Peripheral nerves of third instar larvae expressing KhcGD12278 under the control of Scer\GAL4moody.SPG show bulges.

The amplitude of the evoked excitatory junction potentials (EJP) (measured after activating the A6 abdominal nerve) is dramatically reduced compared to wild type in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4Gli-J29-Gal4. In addition, the response of the muscle is delayed. The amplitude of spontaneous miniature EJPs is normal in these animals.

The amplitude of the evoked excitatory junction potentials (EJP) (measured after activating the A6 abdominal nerve) is dramatically reduced compared to wild type in third instar larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo. In addition, the response of the muscle is delayed. The amplitude of spontaneous miniature EJPs is normal in these animals.

The amplitudes of spontaneous and evoked EJPs (measured after activating the A6 abdominal nerve) are not significantly different to wild-type in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4nrv2.PS.

Expression of KhcGD12278 in adult motoneurons of flies under the control of Scer\GAL4RapGAP1-OK6 lead to the development of unexpanded wings.

Adults expressing KhcGD12278 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 80-90% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 0% or 100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of KhcGD12278 enhances the multiple wing hair phenotype caused by expression of kermitScer\UAS.cLa under the control of Scer\GAL4Bx-MS1096.

Xenogenetic Interactions
Statement
Reference

Co-expression of Dana\KhcRNAi-res.Scer\UAS rescues the locomotor defects seen in adults and larvae expressing KhcGD12278 under the simultaneous control of both Scer\GAL4repo.PL and Scer\GAL4repo.

Co-expression of Dana\KhcRNAi-res.Scer\UAS rescues the reduced excitatory junction potential amplitude seen in third instar larvae expressing KhcGD12278 under the control of Scer\GAL4Gli-J29-Gal4.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
KhcGD12278
Name Synonyms
Secondary FlyBase IDs
    References (11)