FlyBase Allele Report: Dmel\Rbf[GD4484]

General Information

Symbol

Dmel\Rbf^GD4484

Species

D. melanogaster

Name

FlyBase ID

FBal0208242

Feature type

allele

Associated gene

Dmel\Rbf

Associated Insertion(s)

Carried in Construct

P{GD4484}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4484}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4484

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates brain glioma

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2009)

exacerbates cancer

modeled by Myc^UAS.cZa, p53^{RNAi.UAS.RasG12D}

(Diaz et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^yolk

(Longworth et al., 2012)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

visible, with Scer\GAL4^GMR.PF

(Ahlander and Bosco, 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

interommatidial bristle, with Scer\GAL4^GMR.PF

(Ahlander and Bosco, 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Schertel et al., 2015)

Detailed Description

Statement

Reference

Expression of Rbf^GD4484 under the control of Scer\GAL4^Bx-MS1096 results in male flies with weak wing size and patterning defects.

Expression of Rbf^GD4484 under the control of Scer\GAL4^Bx-MS1096 results in female flies with weak wing size and patterning defects.

(Schertel et al., 2015)

Flies expressing Rbf^GD4484 under the control of Scer\GAL4^yolk show reduced survival compared to wild-type controls after infection with S. aureus. Survival rates after infection with P. aeruginosa is normal in the mutant flies.

(Longworth et al., 2012)

Adults expressing Rbf^GD4484 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression of Rbf^GD4484 under the control of Scer\GAL4^GMR.PF results in a modest loss of eye bristles.

(Ahlander and Bosco, 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4484

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^GD4484, Scer\GAL4^GMR.PF has visible phenotype, enhanceable by sqd^GD8593, Scer\GAL4^GMR.PF

(Ahlander and Bosco, 2009)

Enhancer of

Statement

Reference

Rbf^GD4484, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, sqd^GD8593

(Ahlander and Bosco, 2009)

Rbf^GD4484, Scer\GAL4^repo.PU is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Rbf^GD4484, Scer\GAL4^repo.PU is an enhancer of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Other

Statement

Reference

Dcr-2^UAS.cDa, Rbf^GD4484, Scer\GAL4^en-e16E has visible phenotype

(Liang et al., 2014)

Dcr-2^UAS.cDa, Rbf^GD4484, Scer\GAL4^en-e16E has abnormal size phenotype

(Liang et al., 2014)

Rbf^GD4484, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has abnormal neuroanatomy | third instar larval stage phenotype

(Read et al., 2009)

Rbf^GD4484, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has neoplasia | third instar larval stage phenotype

(Read et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rbf^GD4484, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by sqd^GD8593, Scer\GAL4^GMR.PF

(Ahlander and Bosco, 2009)

Enhancer of

Statement

Reference

Rbf^GD4484, Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Scer\GAL4^GMR.PF, sqd^GD8593

(Ahlander and Bosco, 2009)

Rbf^GD4484, Scer\GAL4^repo.PU is an enhancer of glial cell | third instar larval stage | increased number phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2009)

Other

Statement

Reference

Rbf^GD4484, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has glial cell | increased number | third instar larval stage phenotype

(Read et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of both Rbf^GD4484 and sqd^GD8593 under the control of Scer\GAL4^GMR.PF results in an extensive loss of eye bristles and the eyes have a flattened appearance.

(Ahlander and Bosco, 2009)

Co-expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Rbf^GD4484 induces excess glia in third instar larval brains.

Expression of Rbf^GD4484 enhances the glial neoplasia seen in third instar larvae when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU. These animals display exacerbated glial neoplasia, with a substantial increase in small anaplastic-like glia throughout the CNS.

(Read et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v10696

w¹¹¹⁸; P{GD4484}v10696

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4484

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Rbf^GD4484

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close