Adulthood-only expression of Sox100B^GD4588 under the control of Scer\GAL4^NP5130 (and GAL80[ts] for the temporal control of expression) induces a decrease in mitotic index in the midgut, even in the setting of regenerative proliferation upon injury by DSS treatment. DSS treatment induces fewer ISC pairs than controls.

Adulthood-generated midgut clones expressing Sox100B^GD4588 under the control of Scer\GAL4^Act.PU are smaller than control clones, mostly composed of enteroblasts, with few ISCs and enterocytes.

Expression of Sox100B^GD4588 under the control of Scer\GAL4^Su(H).GBE results in adult midgut EBs failing to differentiate into ECs.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, Sox100B^GD4588 has visible phenotype

Dcr-2^UAS.cDa, Scer\GAL4^en-e16E, Sox100B^GD4588 has abnormal size phenotype

Scer\GAL4^Su(H).GBE, Sox100B^GD4588 has enteroblast | increased number | adult stage phenotype, suppressible by Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa

Scer\GAL4^Su(H).GBE, Sox100B^GD4588 has adult midgut enterocyte | decreased number phenotype, suppressible by Scer\GAL4^Su(H).GBE/Sox21a^UAS.cCa