FlyBase Allele Report: Dmel\Sox14[GD4416]

General Information

Symbol

Dmel\Sox14^GD4416

Species

D. melanogaster

Name

FlyBase ID

FBal0208581

Feature type

allele

Associated gene

Dmel\Sox14

Associated Insertion(s)

Carried in Construct

P{GD4416}

Also Known As

UAS-Sox14 RNAi, Sox14-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4416}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4416

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite, with Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012, Kirilly et al., 2009)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012, Kirilly et al., 2009)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Sox14^GD4416 under the control of Scer\GAL4^ppk.1.9 results in dendrite severing defects in the ddaC dorsal dendritic arborising neurons at 18 hours after puparium formation.

(Loncle and Williams, 2012)

Adults expressing Sox14^GD4416 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

A Sox14^GD4416 mutant background (under the control of Scer\GAL4^ppk.1.9) does not affect the overall morphology of dendritic arbors. Notably however, at 18 hours APF, approximately nine primary and secondary dendrites are still attached to the soma, whereas no dendrites are observed in wild-type ddaC neurons.

Knockdown of Sox14 by expression of Sox14^GD4416 using the dendritic arborization neuron driver Scer\GAL4^109(2)80 inhibits neuronal death in the majority of ddaF, ddaA and ddaB neurons at 18h APF.

(Kirilly et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in strong thorax closure defects.

Expression under the control of Scer\GAL4^pnr-MD237 results in an enlarged notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4416

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.1.9, Sox14^GD4416 has abnormal neuroanatomy phenotype, non-suppressible by Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Sox14^GD4416, Scer\GAL80^ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Sox14^GD4416, Scer\GAL80^ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.1.9, Sox14^GD4416 has larval dorsal multidendritic neuron ddaC phenotype, non-suppressible by Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Scer\GAL4^ppk.1.9, Sox14^GD4416 has dendrite phenotype, non-suppressible by Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hdc^Scer\UAS.cSa does not suppress the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing Sox14^GD4416 under the control of Scer\GAL4^ppk.1.9 at 18 hours after puparium formation.

(Loncle and Williams, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Sox14^GD4416 is rescued by Sox14^fl.UAS/Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Comments

Co-expression of Sox14^fl.Scer\UAS rescues the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing Sox14^GD4416 under the control of Scer\GAL4^ppk.1.9 at 18 hours after puparium formation.

(Loncle and Williams, 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v10856

w¹¹¹⁸; P{GD4416}v10856/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts