Expression of Sox14GD4416 under the control of Scer\GAL4ppk.1.9 results in dendrite severing defects in the ddaC dorsal dendritic arborising neurons at 18 hours after puparium formation.
Adults expressing Sox14GD4416 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
A Sox14GD4416 mutant background (under the control of Scer\GAL4ppk.1.9) does not affect the overall morphology of dendritic arbors. Notably however, at 18 hours APF, approximately nine primary and secondary dendrites are still attached to the soma, whereas no dendrites are observed in wild-type ddaC neurons.
Knockdown of Sox14 by expression of Sox14GD4416 using the dendritic arborization neuron driver Scer\GAL4109(2)80 inhibits neuronal death in the majority of ddaF, ddaA and ddaB neurons at 18h APF.