FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Cul2GD8913
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General Information
Symbol
Dmel\Cul2GD8913
Species
D. melanogaster
Name
FlyBase ID
FBal0209308
Feature type
allele
Associated gene
Dmel\Cul2
Associated Insertion(s)
Carried in Construct
P{GD8913}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD8913}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD8913
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU driver display defects in egg chamber development with extra nurse cells compared to controls.

      Majority of germaria from adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU display increased number of spectrosome-containing cells and cells recognized by activated Casp3 antibody compared to controls.

      Young females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU are partially sterile, while older females almost always lack ovaries altogether.

      Adult females expressing Cul2GD8913 RNAi under the control of either the Scer\GAL4dpp.PU or the Scer\GAL4en.PU driver display weak germaria phenotype (increase in the number of spectrosome containing cells compared to controls) with low penetrance. Stronger phenotype with higher penetrance is seen with the Scer\GAL4bab1-Gal4.

      Adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4tj.PU driver have no ovaries.

      Adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4C587 driver show no abnormalities either in germaria development or in the total number of somatic cells they contain.

      The number of cap cells in germaria from adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU does not change compared to controls.

      Flies expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU die if reared at 29oC.

      (Ayyub et al., 2015)

      Larvae expressing Cul-2GD8913 under the control of Scer\GAL4elav.PU show a significant increase in bouton number (counted from muscles 6 and 7 of abdominal segment A2), compared to controls.

      (Ayyub, 2011)

      Expression under the control of Scer\GAL41407 results in an increase in the average size of neuroblasts in the larval brain compared to controls.

      Expression under the control of Scer\GAL41407 results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

      Expression under the control of Scer\GAL41407 results in mild defects in the shape of neuroblasts in the larval brain.

      (Neumüller et al., 2011)

      Adults expressing Cul2GD8913 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      Expression under the control of Scer\GAL4Mef2.PR results in early pupal lethality.

      (Schnorrer et al., 2010)

      Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.

      Expression under the control of Scer\GAL4pnr-MD237 results in loss of bristles on the notum in 30-40% of the Scer\GAL4pnr-MD237 expression domain.

      Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 60-70% of the Scer\GAL4pnr-MD237-expressing area of the notum.

      (Mummery-Widmer et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      Cul2GD8913, Scer\GAL4elav.PU is a suppressor of abnormal neuroanatomy phenotype of Cul5EY00051, Scer\GAL4elav.PU

      (Ayyub, 2011)
      NOT Suppressor of
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Cul2GD8913, Scer\GAL4GMR.PU is a non-suppressor of eye | progressive phenotype of Hsap\ATXN3tr.Q78.UAS.Tag:HA, Scer\GAL4GMR.PU

      (Chen et al., 2019)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The increased number of spectrosome-containing cells in germaria characteristic for adult females expressing Cul2GD8913 RNAi under the control of the Scer\GAL4ptc.PU can be partially rescued by combination with either dpphr92, dpphr56 or tkv7 in a heterozygous state or by knocking-down dpp by co-expression of either dppJF01090 or dppJF01091 RNAi.

      (Ayyub et al., 2015)

      Co-expression of Cul-2GD8913 decreases the number of boutons seen by Scer\GAL4elav.PU-mediated expression of Cul-5EY00051 to levels seen by expression of Cul-2GD8913 alone.

      (Ayyub, 2011)
      Xenogenetic Interactions
      Statement
      Reference

      Adults co-expressing Hsap\ATXN3tr.Q27.UAS.Tag:HA and Cul2GD8913 under the control of Scer\GAL4GMR.PU do not present any obvious eye defects.

      (Chen et al., 2019)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Neuroblasts Screen Database (Knoblich Lab) - A database for RNAi phenotypes in larval neural stem cells
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cul-2GD8913
      Cul2GD8913
      cul-2GD8913
      Name Synonyms
      Secondary FlyBase IDs
        References (13)