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General Information
Symbol
Dmel\mysGD15002
Species
D. melanogaster
Name
FlyBase ID
FBal0210155
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-mys-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of mysGD15002 under the control of Scer\GAL4hth-GETDB in larval antennal discs does not lead to any obvious defects in the formation of the A1 fold or segregation of cells with respect to the field boundary at the A1 fold, but mutant cells exhibit some morphological defects, such as swelling, enlargement, or delamination, as compared to controls.

Scer\GAL4nSyb.PS-driven, presynaptic expression of mysGD15002 blocks activity-induced (upon high K[+] stimulation) enlargement of neuromuscular junction boutons in third instar larvae whereas Scer\GAL4how-24B-controlled (combined with tub-Gal80[ts] to limit the expression to larval stage and avoid lethality), postsynaptic expression does not.

Expressing mysGD15002 under the control of Scer\GAL4repo (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in decreased glial cell numbers in the eye discs of second and third instar larvae. Photoreceptor axon targeting to the optic stalk is unaffected.

Expression of mysGD15002 RNAi under the control of Scer\GAL4pnr-MD237 (but not Scer\GAL4A58) results in large syncitia being formed both at boundaries and intrasegmentally (reflecting the gradual spatial restriction of the driver expression pattern) in third instar larval epidermis and also leads to detachment of muscle fibers from tendon cells.

Expression of mysGD15002 under the control of Scer\GAL4Hand.PA results in a severe defect in the adult heart muscle, with the cardiomyocytes showing a 'rounded-up' phenotype.

Expression of mysGD15002 under the control of Scer\GAL4NP5130, in combination with a Gal80[ts] transgene to restrict expression to the two weeks of adulthood prior to analysis, results in a reduction in Esg-positive progenitors, including a reduction in intestinal stem cell numbers, in the adult posterior midgut, with with a decrease in cell division (PH3 staining) and no increase in apoptosis (TUNEL assay).

When mysGD15002 is expressed in third instar larvae under the control of Scer\GAL421-7 (and co-expressed with Dcr-2Scer\UAS.cDa) 24.33% of the length of the ddaC dendrites at the dorsal midline are enclosed in the epidermis rather than attached to the ECM compared to only 5.69% in controls.

Adults expressing mysGD15002 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in embryonic lethality or late larval lethality.

Expression under the control of Scer\GAL4Mef2.PR results in rounded larval body wall muscles.

Expression under the control of Scer\GAL4Mef2.PR results in sarcomere defects in the larval body wall muscles.

Expression under the control of Scer\GAL4pnr-MD237 results in lethality (either before or at the pupal stage), depending on the insertion line used.

Hub cells often fail to localise to the apical tip of the testis in males expressing mysGD15002 under the control of Scer\GAL4C587.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
NOT suppressed by
Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
mysGD15002
β-IntegrinRNAi#1
β-integrinRNAi
Name Synonyms
Secondary FlyBase IDs
    References (18)