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General Information
Symbol
Dmel\tkvGD2549
Species
D. melanogaster
Name
FlyBase ID
FBal0210763
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tkvRNAi, UAS-tkv RNAi
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Adulthood-specific expression of tkvGD2549 under the combined control of Scer\GAL4esg.PU and Scer\GAL80Su(H).GBE (together with Gal80[ts] for the temporal regulation of expression) prevents re-establishment of quiescence (phospho-H3)in the adult gut upon Ecc15 bacterial infection, leading to dysplasia 2 days after infection; upon Pseudomonas entomophila infection, there is an increase in gut barrier dysfunction (in Smurf assays) and increased mortality, as compared to controls.

Adulthood-only expression of tkvGD2549 under the combined control of Scer\GAL4esg.PU and Gal80[ts] (induced by temperature shift from 18[o]C to 29[o]C) leads to the adult posterior midgut showing increased numbers of intestinal stem cells and enteroblasts (i.e. esg-, Dl- and GBE+Su(H)-positive cells), but no changes in the numbers of enteroendocrine cells, and showing increased mitotic index (pH3-positive cells), as compared to controls; the size of the polyploid cells expressing tkvGD2549 is smaller than fully differentiated enterocytes, indicating a defect in maturation.

In wild-type flies, the pH of the anterior and posterior midgut are over 4.6, while in the copper cell region the pH is less than 3.0. In flies expressing tkvGD2549 under the control of both Scer\GAL80ts.αTub84B and Scer\GAL4esg-NP5130, the pH is uniformly above 4.6 throughout the midgut, demonstrating that functional copper cells are not made.

Expression of tkvGD2549 under the control of Scer\GAL4GMR24H06 for 8 days leads to a dramatic increase in mitotic cell numbers in the posterior midgut as compared with sibling co

Expression of tkvGD2549 under the control of Scer\GAL4Myo31DF-NP0001 has no effect on mitotic cell numbers in the posterior midgut as compared to sibling controls.

rols.

Adults expressing tkvGD2549 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 100% of the Scer\GAL4pnr-MD237-expressing area of the notum.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (13)