FlyBase Allele Report: Dmel\trio[GD9531]

General Information

Symbol

Dmel\trio^GD9531

Species

D. melanogaster

Name

FlyBase ID

FBal0210795

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

P{GD9531}

Also Known As

UAS-trio-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9531}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9531

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

abnormal neuroanatomy, with Scer\GAL4^sev.EP

(Astigarraga et al., 2010)

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

decreased size | larval stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

axon | embryonic stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

chaeta, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

chaeta | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

commissure | embryonic stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

connective | embryonic stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

medulla, with Scer\GAL4^sev.EP

(Astigarraga et al., 2010)

NMJ bouton | decreased number | larval stage, with Scer\GAL4^elav.PU

(Wang et al., 2026)

photoreceptor cell R7, with Scer\GAL4^sev.EP

(Astigarraga et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of trio^GD9531 using Scer\GAL4^sev.EP, and in the presence of Dcr-2^Scer\UAS.cDa, results in growth of R7 axons beyond the M6 layer of the medulla.

(Astigarraga et al., 2010)

Adults expressing trio^GD9531 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in a weak flyer phenotype or adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 90-100% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9531

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, trio^GD9531 has abnormal neuroanatomy | larval stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has decreased size | larval stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has abnormal neuroanatomy | embryonic stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has abnormal neuroanatomy | larval stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has decreased size | larval stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of abnormal neuroanatomy | larval stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of abnormal neuroanatomy | larval stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of increased size | larval stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of increased size | larval stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

NOT Suppressor of

Statement

Reference

trio^GD9531/Scer\GAL4^GMR.PFa is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, trio^GD9531 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has NMJ bouton | larval stage | decreased number phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has axon | embryonic stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has connective | embryonic stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has commissure | embryonic stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has axon | embryonic stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has connective | embryonic stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has commissure | embryonic stage phenotype, suppressible by mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has embryonic/larval neuromuscular junction | larval stage phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU, trio^GD9531 has NMJ bouton | larval stage | decreased number phenotype, suppressible by E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of axon | embryonic stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of connective | embryonic stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of commissure | embryonic stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of NMJ bouton | increased number | larval stage phenotype of mir-33^KO

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of commissure | embryonic stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of connective | embryonic stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of axon | embryonic stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of NMJ bouton | increased number | larval stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

Scer\GAL4^elav.PU/trio^GD9531 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of E2f1ⁱ²/E2f1⁰⁷¹⁷²

(Wang et al., 2026)

NOT Suppressor of

Statement

Reference

trio^GD9531/Scer\GAL4^GMR.PFa is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PFa, egr^UAS.cMa

(Ruan et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dot-like small eye phenotype characteristic for flies expressing egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PFa cannot be suppressed by co-expression of trio^GD9531 RNAi.

(Ruan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v40138