FlyBase Allele Report: Dmel\Rab9[Q71L.UASp.YFP]

General Information

Symbol

Dmel\Rab9^{Q71L.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215406

Feature type

allele

Associated gene

Dmel\Rab9

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab9.Q71L}

Key Links

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab9.Q71L}

Cytology

Description

Amino acid replacement: Q71L.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted constitutively active form of Rab9 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab9

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates epilepsy

modeled by para^bss1

(Kroll et al., 2015)

ameliorates brain cancer

modeled by Vps35^E42

(Li et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mesonotum, with Scer\GAL4^pnr-MD237

(Thomas et al., 2009)

Detailed Description

Statement

Reference

Expression via Scer\GAL4^pnr-MD237 results in a naked notum.

(Thomas et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rab9^{Q71L.UASp.YFP} is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{Q71L.UASp.YFP} is a suppressor of increased cell number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a suppressor of abnormal neuroanatomy | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^GMR57C10 is a suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP} is a suppressor | partially of abnormal cell death phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Lrrk^unspecified

(Dodson et al., 2014)

NOT Suppressor of

Statement

Reference

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^GMR57C10 is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/Rab9^{Q71L.UASp.YFP} is a suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a suppressor of larval neuroblast | increased number | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^pnt-14-94 is a suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^insc-Mz1407/Rab9^{Q71L.UASp.YFP} is a suppressor of larval brain | larval stage phenotype of Vps35^E42

(Li et al., 2018)

Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP} is a suppressor of follicle cell phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6 is a suppressor | partially of embryonic/larval neuromuscular junction phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Rab9^{Q71L.UASp.YFP}/Scer\GAL4^RapGAP1-OK6 is a suppressor | partially of NMJ bouton | increased number phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Scer\GAL4^CY2/Rab9^{Q71L.UASp.YFP} is a suppressor | partially of lysosome phenotype of Lrrk^unspecified

(Dodson et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 partially rescues the bang sensitivity of para^bss1/+ flies, but does not rescue the bang sensitivity of hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

Expression of Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^CY2 partially suppresses the lysosome enlargement and premature follicle cell death phenotypes observed in Lrrk mutants.

Expression of Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^RapGAP1-OK6 partially rescues the neuromuscular junction synapse overgrowth phenotype of Lrrk mutants.

(Dodson et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9785

w^*; P{UASp-YFP.Rab9.Q71L}20

23273

y¹ w^*; P{UASp-YFP.Rab9.Q71L}10

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab9^{Q71L.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab9^{Q71L.UASp.YFP}

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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