FlyBase Allele Report: Dmel\Gcn2[KK103566]

General Information

Symbol

Dmel\Gcn2^KK103566

Species

D. melanogaster

Name

FlyBase ID

FBal0232393

Feature type

allele

Associated gene

Dmel\Gcn2

Associated Insertion(s)

Carried in Construct

P{KK103566}

Also Known As

GCN2 RNAi, UAS-Gcn2^RNAi, UAS-Gcn2-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK103566}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK103566

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates retinitis pigmentosa

modeled by ninaE^G69D.UAS

(Kang et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber | decreased number, with Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

oocyte, with Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

stage S14 oocyte | increased number, with Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

yolk granule | decreased number, with Scer\GAL4^Lsp2.3.1

(Grmai et al., 2024)

Detailed Description

Statement

Reference

Flies expressing Gcn2^KK103566 under the control of Scer\GAL4^Act.PU or Scer\GAL4^da.G32 during the adult stage and infected with P. entomophila show an increase in gut stem cell proliferation and in gut repair, in contrast to wild-type flies infected with P. entomophila.

Flies expressing Gcn2^KK103566 under the control of Scer\GAL4^da.G32 during the adult stage are more resistant to P. entomophila infection than control flies.

(Chakrabarti et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Gcn2^KK103566 is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Celardo et al., 2017)

Gcn2^KK103566, Scer\GAL4^109(2)80 is a non-suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Other

Statement

Reference

Gcn2^KK103566, PPP1R15^GD5953, Scer\GAL4^da.G32 has majority die during embryonic stage phenotype

(Malzer et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Gcn2^KK103566, Scer\GAL4^ninaE.PU, Scer\GAL4^GMR.PU is an enhancer of retina | adult stage | progressive phenotype of Scer\GAL4^GMR.PU, Scer\GAL4^ninaE.PU, ninaE^G69D.UAS

(Kang et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav.PU/Gcn2^KK103566 is a non-suppressor of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Celardo et al., 2017)

Gcn2^KK103566, Scer\GAL4^109(2)80 is a non-suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Gcn2^KK103566, Scer\GAL4^109(2)80 is a non-suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Gcn2^KK103566 RNAi driven by Scer\GAL4^elav.PU does not rescue the loss of dopaminergic ppl1 cluster neurons observed in either Pink1^B9 or park²⁵ mutant adults.

(Celardo et al., 2017)

The age-progressive retinal degeneration characteristic for adult flies expressing ninaE^{G69D.Scer\UAS} under the combined control of Scer\GAL4^ninaE.PU and Scer\GAL4^GMR.PU is further worsened by co-expression of Gcn2^KK103566 and unlike in the flies expressing only ninaE^{G69D.Scer\UAS}, the phenotype cannot be ameliorated by keeping the flies on a low-protein diet.

(Kang et al., 2017)

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 is not significantly affected by co-expression of Gcn2^KK103566.

(Tsuyama et al., 2017)

Embryos co-expressing both Gadd34^GD5953 and Gcn2^KK103566 under the control of Scer\GAL4^da.G32 show a strong reduction in the rate of hatching compared to both wild type and embryos expressing either Gadd34^GD5953 or Gcn2^KK103566 singly.

(Malzer et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v103976

P{KK103566}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Gcn2^KK103566

Gcn2[KK]

(Tsuyama et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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