FlyBase Allele Report: Dmel\tra[JF03132]

General Information

Symbol

Dmel\tra^JF03132

Species

D. melanogaster

Name

FlyBase ID

FBal0239631

Feature type

allele

Associated gene

Dmel\tra

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03132}

Also Known As

UAS-tra^RNAi, UAS-traRNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03132}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03132

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sex-determination, with Scer\GAL4^dome-PG14

(Yan and Perrimon, 2015)

abnormal sex-determination | adult stage, with Scer\GAL4^Act5C.PI

(Han et al., 2022)

abnormal size | adult stage | female limited, with Scer\GAL4^NP7397

(Hudry et al., 2016)

abnormal size | adult stage | somatic clone, with Scer\GAL4^NP7397

(Hudry et al., 2016)

decreased cell number | adult stage | female limited, with Scer\GAL4^NP7397

(Hudry et al., 2016)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Hudry et al., 2016)

decreased occurrence of cell division | adult stage | female | conditional, with Scer\GAL4^NP7397

(Hudry et al., 2016)

decreased size | adult stage | female, with Scer\GAL4^GMR11F02

(Hérault et al., 2024)

visible | adult stage, with Scer\GAL4^Act5C.PI

(Han et al., 2022)

visible | adult stage | female, with Scer\GAL4^GMR11F02

(Hérault et al., 2024)

Phenotype Manifest In

Detailed Description

Statement

Reference

Under control conditions, the adulthood-only expression of tra^JF03132 under the control of Scer\GAL4^esg-NP7397 (together with Gal80[ts], for the temporal regulation of expression, and Dicer-2, for efficient RNAi) leads to a significant shortening of the adult midgut in females only, but does not significantly affect the overall cell number, the progenitor cell number (intestinal stem cells/enteroblasts) or the mitotic index in the female or male adult posterior midgut, as compared to controls. This expression also leads to a significant decrease in the number of enteroblasts, but not of intestinal stem cells, and to a decrease in the mitotic index in the adult midgut of mated females, but not of virgin females, as compared to controls.

Under control conditions, the clonal expression of tra^JF03132 under the control of Scer\GAL4^tub.PU leads to a significant decrease in average clone cell number, while and the clonal and adulthood-only expression under the control of Scer\GAL4^esg-NP7397 (together with Gal80[ts], for the temporal regulation of expression) leads to a significant decrease in clonal area, as compared to controls.

(Hudry et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

tra^JF03132, Scer\GAL4^NP7397 is a suppressor of increased occurrence of cell division | female limited | adult stage phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

tra^JF03132/Scer\GAL4^mir-124.4.7 is a suppressor of abnormal mating behavior phenotype of mir-124^Δ4/mir-124^Δ177

(Weng et al., 2013)

tra^JF03132/Scer\GAL4^elav.PU is a suppressor of abnormal mating behavior phenotype of mir-124^Δ4/mir-124^Δ177

(Weng et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

tra^JF03132, Scer\GAL4^NP7397 is a suppressor of adult posterior midgut epithelium | female limited phenotype of N^KK102890, Scer\GAL4^NP7397

(Hudry et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Under control conditions, the adulthood-only co-expression of tra^JF03132 and dsx^UAS.female under the control of Scer\GAL4^esg-NP7397 (together with Gal80[ts], for the temporal regulation of expression, and Dicer-2, for efficient RNAi) does not significantly affect the number of progenitor cells (intestinal stem cells/enteroblasts) or the mitotic index in the female adult posterior midgut, as compared to controls.

The co-expression of tra^JF03132 suppresses the increased mitotic index in the female adult posterior midgut induced by the adulthood-only expression of N^KK102890 under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression).

(Hudry et al., 2016)

Expression of tra^JF03132 under the control of Scer\GAL4^mir-124.4.7 significantly increases male-female mating success, reduces male-male courtship, improves production of cis-vaccenyl acetate and lowers pentacosene levels in mir-124^Δ4/mir-124^Δ177 males.

Expression of tra^JF03132 under the control of Scer\GAL4^elav.PU suppresses male-male courtship in mir-124^Δ4/mir-124^Δ177 males.

(Weng et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28512

y¹ v¹; P{TRiP.JF03132}attP2

Notes on Origin

Discoverer

Comments

Supplemental Dataset_S01.

(Yan and Perrimon, 2015)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tra^JF03132

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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