FlyBase Allele Report: Dmel\ptc[JF03223]

General Information

Symbol

Dmel\ptc^JF03223

Species

D. melanogaster

Name

FlyBase ID

FBal0239721

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

Carried in Construct

P{TRiP.JF03223}

Also Known As

ptc-RNAi, ptc^RNAi, ptc RNAi, UAS-ptc RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF03223}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF03223

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority live, with Scer\GAL4^4XHand

(Zhu et al., 2017)

visible, with Scer\GAL4^ptc.PU

(Kim et al., 2014)

Phenotype Manifest In

anterior-posterior compartment boundary of the wing blade, with Scer\GAL4^ptc.PU

(Kim et al., 2014)

basal stalk, with Scer\GAL4^109-30

(Ulmschneider et al., 2016)

wing blade, with Scer\GAL4^ptc.PU

(Kim et al., 2014)

wing disc | somatic clone, with Scer\GAL4^unspecified

(Kagey et al., 2012)

Detailed Description

Statement

Reference

The expression of ptc^JF03223 under the control of Scer\GAL4^109-30 leads to ovarioles frequently exhibiting multilayered stalks, as compared to controls.

(Ulmschneider et al., 2016)

Expression of ptc^JF03223 driven by Scer\GAL4^ptc.PU causes overgrowth of the A-P boundary, increasing distance between the L3 and L4 wing veins.

(Kim et al., 2014)

Expression of ptc^JF03223 in clones in the wing disc results in overgrowth of the wing disc.

(Kagey et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, ptc^JF03223 has visible phenotype, suppressible by mir-14^{UAS.cLa.DsRed(Unk)}/Scer\GAL4^ptc.PU

(Kim et al., 2014)

Other

Statement

Reference

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^109-30, ptc^JF03223 has basal stalk phenotype, suppressible | partially by Nhe2^UAS.cGa, Scer\GAL4^109-30

(Ulmschneider et al., 2016)

Scer\GAL4^ptc.PU, ptc^JF03223 has wing blade phenotype, suppressible by mir-14^{UAS.cLa.DsRed(Unk)}/Scer\GAL4^ptc.PU

(Kim et al., 2014)

Scer\GAL4^ptc.PU, ptc^JF03223 has anterior-posterior compartment boundary of the wing blade phenotype, suppressible by mir-14^{UAS.cLa.DsRed(Unk)}/Scer\GAL4^ptc.PU

(Kim et al., 2014)

Scer\GAL4^unspecified, ptc^JF03223 has wing disc | somatic clone phenotype, suppressible by yki[+]/yki^B5

(Kagey et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^109-30, ptc^JF03223 has basal stalk phenotype, non-suppressible by Nhe2^{UAS.E358I.Tag:HA}, Scer\GAL4^109-30

(Ulmschneider et al., 2016)

Scer\GAL4^109-30, ptc^JF03223 has basal stalk phenotype, non-suppressible by Nhe2^HMC03243, Scer\GAL4^109-30

(Ulmschneider et al., 2016)

Other

Statement

Reference

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, ptc^JF03223 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The basal stalk multilayering observed upon the expression of ptc^JF03223 under the control of Scer\GAL4^109-30 is partially suppressed by the co-expression of Nhe2^UAS.cGa, but not of Nhe2^{UAS.E358I.Tag:HA} or Nhe2^HMC03243.

(Ulmschneider et al., 2016)

Expression of ptc^JF03223 under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background suppresses the recovery of spectrosome-containing cells at 48 h after heat shock seen in flies expressing bam^hs.PO alone in a bam^Δ86/bam^Δ86 background.

(Liu et al., 2015)

Co-expression of mir-14^{Scer\UAS.cLa.T:Disc\RFP} suppresses the increased distance between the L3 and L4 wing veins seen in Scer\GAL4^ptc.PU>ptc^JF03223 flies; these flies even have a partial reduction in the distance between L3 and L4 (towards Scer\GAL4^ptc.PU>mir-14^{Scer\UAS.cLa.T:Disc\RFP} phenotypes, with a slight curving of the L4 vein towards the anterior portion of the wing).

(Kim et al., 2014)

yki^B5/+ suppresses the wing disc overgrowth caused by expression of ptc^JF03223 in clones in the wing disc.

(Kagey et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28795

y¹ v¹; P{TRiP.JF03223}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ptc^BL28795

(Liu et al., 2015)

ptc^JF03223

ptc^RNAi

(Chai et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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