FlyBase Allele Report: Dmel\brm[HM04019]

General Information

Symbol

Dmel\brm^HM04019

Species

D. melanogaster

Name

FlyBase ID

FBal0245321

Feature type

allele

Associated gene

Dmel\brm

Associated Insertion(s)

Carried in Construct

P{TRiP.HM04019}

Also Known As

UAS-brm-RNAi, Brm RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

(Ni et al., 2008)

Progenitor genotype

Carried in construct

P{TRiP.HM04019}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Ni et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HM04019

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

abnormal size | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

decreased cell number | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2013)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019)

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Jin et al., 2013)

lethal, with Scer\GAL4^Act5C.PI

(Dietz et al., 2015)

partially lethal - majority die, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

adult midgut epithelium | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019)

eye, with Scer\GAL4^ey.PH

(Dietz et al., 2015)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Jin et al., 2013)

wing, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

wing, with Scer\GAL4^en.PU

(Dietz et al., 2015)

wing blade, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Detailed Description

Statement

Reference

Somatic clones expressing brm^HM04019 under the control of Scer\GAL4^Tub.PU contain a smaller overall number of cells and intestinal stem cells in adult flies compared to controls.

(Gervais et al., 2019)

Expression of brm^HM04019 under the control of Scer\GAL4^C587 does not cause any obvious defects in germaria morphology and development.

(Li et al., 2016)

Expression of brm^HM04019 results in: severely reduced eye size (with Scer\GAL4^ey.PH), small and deformed or blistered wings (with Scer\GAL4^nub.PK) and near complete pupal lethality - the few escapers display wing phenotype (with Scer\GAL4^en.PU). Scer\GAL4^Act5C.PI-driven expression is lethal; no overt adult phenotype is observed with Scer\GAL4^GMR.PF.

(Dietz et al., 2015)

Expression of brm^HM04019 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} does not result in melanisation of the fat body.

(Zang et al., 2015)

Fewer intestinal stem cells (ISCs) are seen in the posterior midguts of adult flies expressing brm^HM04019 under the control of Scer\GAL4^esg-NP5130. The number of mitotic cells is also reduced. The cells exhibit an spherical shape compared with the angular shaped control cells.

(Jin et al., 2013)

Expression of brm^HM04019 under the control of Scer\GAL4^Bx-MS1096 results in a reduction in wing size, slight curling of the wing and an abnormal wing shape.

(Friedman et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PK, brm^HM04019 has visible | adult stage phenotype, enhanceable by Scer\GAL4^nub.PK/htt^GD14943

(Dietz et al., 2015)

Scer\GAL4^nub.PK, brm^HM04019 has visible | adult stage phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Suppressor of

Statement

Reference

brm^HM04019, Scer\GAL4^C587 is a suppressor | partially of increased cell number | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

brm^HM04019, Scer\GAL4^C587 is a suppressor | partially of neoplasia | female | adult stage phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PK, brm^HM04019 has wing blade phenotype, enhanceable by Scer\GAL4^nub.PK/htt^GD14943

(Dietz et al., 2015)

Scer\GAL4^nub.PK, brm^HM04019 has wing blade phenotype, enhanceable by htt^int

(Dietz et al., 2015)

Suppressor of

Statement

Reference

brm^HM04019, Scer\GAL4^C587 is a suppressor | partially of germarium phenotype of Psc^GD4481, Scer\GAL4^C587, Su(z)2^GD16388

(Li et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous expression of both Psc^GD4481 and Su(z)2^GD16388 under the control of Scer\GAL4^C587 (together with tub-Gal80[ts] to limit the time of expression to adulthood) results in tumorigenic accumulation of spectrosome containing germline stem cell-like (GSC-like) cells (which increase in number with the time of expression) in female germaria; this accumulation of GSC-like cells is significantly suppressed by co-expression of brm^HM04019.

(Li et al., 2016)

The adult wing phenotype induced by expression of brm^HM04019 under the control of Scer\GAL4^nub.PK is enhanced by co-expression of htt^GD14943 or by combination with a single copy of htt^int.

(Dietz et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31712

y¹ v¹; P{TRiP.HM04019}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

brm^HM04019

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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