FlyBase Allele Report: Dmel\grim[UAS.cUa]

General Information

Symbol

Dmel\grim^UAS.cUa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Unknown

FlyBase ID

FBal0249395

Feature type

allele

Associated gene

Dmel\grim

Associated Insertion(s)

Carried in Construct

P{UAS-grim.U}

Also Known As

UAS-grim

Key Links

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-grim.U}

Cytology

Description

UAS regulatory sequences drive expression of grim.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

grim

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by dlg1^A40.2

(Parisi et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage, with Scer\GAL4^34B

(Li et al., 2022)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

decreased body size | adult stage, with Scer\GAL4^34B

(Li et al., 2022)

decreased body size | pupal stage | nutrition conditional, with Scer\GAL4^34B

(Li et al., 2022)

decreased body weight | adult stage, with Scer\GAL4^34B

(Li et al., 2022)

decreased cell number | larval stage, with Scer\GAL4^eg-Mz360

(Pérez et al., 2022)

decreased size | adult stage, with Scer\GAL4^34B

(Li et al., 2022)

decreased size | larval stage, with Scer\GAL4^34B

(Li et al., 2022)

increased cell death | somatic clone | third instar larval stage, with Scer\GAL4^btl.PU

(Burguete et al., 2019)

lethal, with Scer\GAL4^c42

(Shukla and Tapadia, 2011)

lethal, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

lethal - all die before end of P-stage, with Scer\GAL4^GMR.PF

(Dwivedi et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

some die during pupal stage, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

visible, with Scer\GAL4^sev.EP

(Dwivedi et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Phenotype Manifest In

embryonic/larval salivary gland | larval stage, with Scer\GAL4^34B

(Li et al., 2022)

eye, with Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

eye, with Scer\GAL4^sev.EP

(Dwivedi et al., 2015)

larval brain | larval stage, with Scer\GAL4^34B

(Li et al., 2022)

larval fat cell, with Scer\GAL4^34B

(Li et al., 2022)

larval ventral nerve cord, with Scer\GAL4^eg-Mz360

(Pérez et al., 2022)

terminal tracheal cell | somatic clone | third instar larval stage, with Scer\GAL4^btl.PU

(Burguete et al., 2019)

wing, with Scer\GAL4^34B

(Li et al., 2022)

wing disc, with Scer\GAL4^34B

(Li et al., 2022)

Detailed Description

Statement

Reference

Embryos expressing grim^UAS.cUa under the control of Scer\GAL4^tup.AME exhibit gonads with similar number of niche aggregates as controls.

(Anllo et al., 2019)

Inducing cell death in somatic clones of terminal tracheal cells by expressing grim^UAS.cUa under the control of Scer\GAL4^btl.PU results in blebbing of the cytoplasm, loss of gass-filling and branch loss in third instar larvae.

(Burguete et al., 2019)

Expression of grim^Scer\UAS.cUa under the control of Scer\GAL4^sev.EP results in a rough eye phenotype with disruption of ommatidial arrangement.

(Dwivedi et al., 2015)

When grim^Scer\UAS.cUa is expressed under the control of Scer\GAL4^GMR.PF at 18[o]C only 0.01% of pupae eclose. Eye size is reduced compared to controls.

(Pechkovsky et al., 2013)

Expression of grim^Scer\UAS.cUa under the control Scer\GAL4^c42 results in lethality during embryonic and first instar larval stages.

(Shukla and Tapadia, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, grim^UAS.cUa has decreased cell number | larval stage phenotype, suppressible | partially by Scer\GAL4^eg-Mz360/hb^UAS.cWa

(Pérez et al., 2022)

Scer\GAL4^GMR.PF, grim^UAS.cUa has lethal phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, grim^UAS.cUa has lethal - all die before end of pupal stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, grim^UAS.cUa has some die during pupal stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, grim^UAS.cUa has visible | adult stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Scer\GAL4^GMR.PF, grim^UAS.cUa has abnormal size | adult stage phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^Hml.Δ/grim^UAS.cUa is an enhancer of neoplasia | larval stage phenotype of dlg1^A40.2

(Parisi et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ/grim^UAS.cUa is a suppressor | partially of increased cell number | larval stage phenotype of dlg1^A40.2

(Parisi et al., 2014)

Other

Statement

Reference

Scer\GAL4^Hml.Δ, dlg1^A40.2, grim^UAS.cUa has decreased cell death | larval stage phenotype

(Parisi et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, grim^UAS.cUa has larval ventral nerve cord phenotype, suppressible | partially by Scer\GAL4^eg-Mz360/hb^UAS.cWa

(Pérez et al., 2022)

Scer\GAL4^GMR.PF, grim^UAS.cUa has eye phenotype, suppressible | partially by HAdV-C\E4orf4^UAS.cPa, Scer\GAL4^GMR.PF

(Pechkovsky et al., 2013)

Enhancer of

Statement

Reference

Scer\GAL4^Hml.Δ/grim^UAS.cUa is an enhancer of wing disc phenotype of dlg1^A40.2

(Parisi et al., 2014)

Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ/grim^UAS.cUa is a suppressor | partially of embryonic/larval hemocyte | larval stage phenotype of dlg1^A40.2

(Parisi et al., 2014)

Other

Statement

Reference

Scer\GAL4^Hml.Δ, dlg1^A40.2, grim^UAS.cUa has wing disc phenotype

(Parisi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The apoptosis observed in dlg1^A40.2 mutant wing disc tumors is significantly decreased by the expression of grim^UAS.cUa under the control of Scer\GAL4^Hml.Δ.

(Parisi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Expression of Zzzz\E4orf4^Scer\UAS.cPa suppresses the semi-lethality seen when grim^Scer\UAS.cUa is expressed under the control of Scer\GAL4^GMR.PF at 18[o]C. 78% of pupae eclose (compared to 0.01%). The reduction in eye size is also partially suppressed.

(Pechkovsky et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

grim^Scer\UAS.cUa

grim^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Unknown

Secondary FlyBase IDs

References (14)

Report Sections

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