FlyBase Allele Report: Scer\GAL4[GMR47A04]

General Information

Symbol

Scer\GAL4^GMR47A04

Species

S. cerevisiae

Name

FlyBase ID

FBal0252806

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR47A04-GAL4}

Also Known As

R47A04-Gal4, 47A04-Gal4, R47A04

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR47A04-GAL4}

Cytology

Description

A 819 base pair fragment from the flanking non-coding or intronic region of Oamb is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR47A04

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

adult fruitless aSP-a (male) neuron

(Watanabe et al., 2017)

adult stage | female & oogenesis

follicle cell

(Liao and Nässel, 2020)

adult stage | female & oogenesis stage S14

follicle cell

Comment: late stage S14 only

(Deady and Sun, 2015, Deady et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^GMR47A04 does not drive expression in adult fruitless aSP-a (female) neurons.

(Watanabe et al., 2017)

ScerGAL4^GMR47A04 drives expression specifically in the follicle cells of mature (stage S14) egg chambers.

(Deady et al., 2015)

ScerGAL4^GMR47A04 drives expression only in late stage S14 follicle cells.

(Deady and Sun, 2015)

Marker for

Reflects expression of

Oamb

(Watanabe et al., 2017, Deady and Sun, 2015, Deady et al., 2015)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oviposition, with peb^GD1507

(Deady et al., 2017)

abnormal oviposition, with peb^KK107575

(Deady et al., 2017)

decreased fecundity | female, with Mmp2^RNAi.UAS

(Deady et al., 2015)

decreased fecundity | female, with Timp^UAS.cPa

(Deady et al., 2015)

female semi-fertile, with dib^KK106954

(Deady et al., 2015)

female semi-fertile, with phtm^KK103215

(Deady et al., 2015)

female semi-fertile, with shd^GD8033

(Deady et al., 2015)

female semi-sterile, with Oamb^GD696

(Deady and Sun, 2015)

female semi-sterile, with Oamb^JF01673

(Deady and Sun, 2015)

female semi-sterile, with Oamb^JF01732

(Deady and Sun, 2015)

female semi-sterile, with Oamb^KK111456

(Deady and Sun, 2015)

Phenotype Manifest In

egg chamber, with Mmp1^f1.UAS

(Deady et al., 2015)

egg chamber, with Mmp2^UAS.cPa

(Deady et al., 2015)

egg chamber | oogenesis stage S14, with Oamb^UAS.K3

(Deady et al., 2017)

egg chamber | oogenesis stage S14, with peb^EP55

(Deady et al., 2017)

egg chamber | oogenesis stage S14, with peb^GD1507

(Deady et al., 2017)

egg chamber | oogenesis stage S14, with peb^KK107575

(Deady et al., 2017)

follicle cell, with Mmp1^f1.UAS

(Deady et al., 2015)

follicle cell, with Mmp2^UAS.cPa

(Deady et al., 2015)

oocyte associated follicular epithelium | oogenesis stage S14, with Oamb^UAS.K3

(Deady et al., 2017)

oocyte associated follicular epithelium | oogenesis stage S14, with peb^EP55

(Deady et al., 2017)

oocyte associated follicular epithelium | oogenesis stage S14, with peb^GD1507

(Deady et al., 2017)

oocyte associated follicular epithelium | oogenesis stage S14, with peb^KK107575

(Deady et al., 2017)

ovarian basement membrane | oogenesis stage S14, with Mmp2^RNAi.UAS

(Deady et al., 2017)

ovarian basement membrane | oogenesis stage S14, with peb^GD1507

(Deady et al., 2017)

ovarian basement membrane | oogenesis stage S14, with peb^KK107575

(Deady et al., 2017)

ovarian basement membrane | oogenesis stage S14, with Timp^UAS.cPa

(Deady et al., 2017)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR47A04, peb^GD1507 has egg chamber | oogenesis stage S14 phenotype, suppressible | partially by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Scer\GAL4^GMR47A04, peb^GD1507 has oocyte associated follicular epithelium | oogenesis stage S14 phenotype, suppressible | partially by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Scer\GAL4^GMR47A04, peb^KK107575 has egg chamber | oogenesis stage S14 phenotype, suppressible by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Scer\GAL4^GMR47A04, peb^KK107575 has oocyte associated follicular epithelium | oogenesis stage S14 phenotype, suppressible by Oamb^UAS.K3, Scer\GAL4^GMR47A04

(Deady et al., 2017)

Suppressor of

Statement

Reference

Oamb^UAS.K3, Scer\GAL4^GMR47A04 is a suppressor | partially of egg chamber | oogenesis stage S14 phenotype of Scer\GAL4^GMR47A04, peb^GD1507

(Deady et al., 2017)

Oamb^UAS.K3, Scer\GAL4^GMR47A04 is a suppressor | partially of oocyte associated follicular epithelium | oogenesis stage S14 phenotype of Scer\GAL4^GMR47A04, peb^GD1507

(Deady et al., 2017)

Oamb^UAS.K3, Scer\GAL4^GMR47A04 is a suppressor of egg chamber | oogenesis stage S14 phenotype of Scer\GAL4^GMR47A04, peb^KK107575

(Deady et al., 2017)

Oamb^UAS.K3, Scer\GAL4^GMR47A04 is a suppressor of oocyte associated follicular epithelium | oogenesis stage S14 phenotype of Scer\GAL4^GMR47A04, peb^KK107575

(Deady et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

peb^EP55/Scer\GAL4^GMR47A04 partially rescues peb^GD1507

(Deady et al., 2017)

Fails to rescue

peb^EP55/Scer\GAL4^GMR47A04 fails to rescue peb^KK107575

(Deady et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

50286

w¹¹¹⁸; P{GMR47A04-GAL4}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^GMR47A04

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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