FlyBase Allele Report: Scer\GAL4[VT005534]

General Information

Symbol

Scer\GAL4^VT005534

Species

S. cerevisiae

Name

FlyBase ID

FBal0256978

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VT005534-GAL4}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Meadows, 2015.3.13)

Progenitor genotype

Carried in construct

P{VT005534-GAL4}

Cytology

Description

A 2075 base pair fragment from the flanking non-coding or intronic region of Nos is fused upstream of a Drosophila synthetic core promoter (DSCP) followed by sequence encoding a GAL4 driver.

(Meadows, 2015.3.13)

Allele components

Component

Use(s)

Regulatory region(s)

VT005534

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

adult fruitless vPR6 (male) neuron | subset

(von Philipsborn et al., 2011)

Additional Information

Statement

Reference

ScerGAL4^VT005534 drives expression in 2-5 dsx-positive adult fruitless vPR6 (male) neurons per hemisphere.

(von Philipsborn et al., 2011)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal song, with Ctet\tetX^{TNT-LC.FRT.stop.UAS}, Ecol\lexA^GMR24B02, Scer\FLP1^L.8xlexAop

(Shirangi et al., 2016)

abnormal song | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.20xUAS.FRT.Venus}, Ecol\lexA^GMR24B02, Scer\FLP1^L.8xlexAop

(Shirangi et al., 2016)

abnormal song | heat sensitive, with Ctet\tetX^{TNT-LC.UAS.cSa}

(von Philipsborn et al., 2011)

abnormal song | heat sensitive, with TrpA1^{-FRT.UAS.cvPa}

(von Philipsborn et al., 2011)

Phenotype Manifest In

Detailed Description

Statement

Reference

Inhibition (using Ctet\TeTxLC^FRT.stop.UAS) of Scer\GAL4^VT005534 neurons expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^GMR24B02 in males results in a slight reduction in sine song during courtship than controls and a significant increase in pulse carrier frequency, but no difference in inter-pulse interval, pulse amplitude, sine amplitude or sine carrier frequency, compared to controls.

Acute optogenetic activation (using Cnoc\ChR1^{Cs.20xUAS.FRT.Venus} with 660 nm (red) light activation) of Scer\GAL4^VT005534 neurons expressing Scer\FLP1^L.8xlexAop under the control of Ecol\lexA^GMR24B02 in males, in the absence of females, induces pulse song, but not sine song.

(Shirangi et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Korea

12315

P{VT005534-GAL4}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^VT005534

Scer\GAL4^VT5534

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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