FlyBase Allele Report: Dmel\Gk1[RNAi.UAS]

General Information

Symbol

Dmel\Gk1^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0268039

Feature type

allele

Associated gene

Dmel\Gk1

Associated Insertion(s)

Carried in Construct

P{UAS-Gk1.RNAi}

Also Known As

dGyk-IR

Key Links

Transgenic product class

RNAi_reagent

(Wightman et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wightman et al., 2012)

Mutagen

in vitro construct

(Wightman et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Gk1.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of Gk1 sequences.

(Wightman et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gk1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of glycerol kinase deficiency

CEA

(Wightman et al., 2012)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

melanotic mass | third instar larval stage, with Scer\GAL4^c564

(Wightman et al., 2013)

wing | pharate adult stage, with Scer\GAL4^c564

(Wightman et al., 2013)

Detailed Description

Statement

Reference

Expression of Gk1^{dsRNA.Scer\UAS} driven by Scer\GAL4^αTub84B.PL results in larval lethality ('Gk2^{dsRNA.Scer\UAS}-let') or survival to adulthood ('Gk1^{dsRNA.Scer\UAS}-sur'), depending on the insertion line. Expression of Gk1^{dsRNA.Scer\UAS}-let (but not Gk1^{dsRNA.Scer\UAS}-sur) lines driven by Scer\GAL4^c564 or Scer\GAL4^how-24B also results in larval and pupal lethality. Scer\GAL4^c564>Gk1^{dsRNA.Scer\UAS}-let third instar larvae often show melanotic masses before lethality mainly at the pharate adult stage (escapers have a curled or crumpled wing phenotype). Expression of Gk1^{dsRNA.Scer\UAS} driven by Scer\GAL4^GMR.PS or Scer\GAL4^elav-C155 does not result in any obvious external or behavioral phenotypes.

(Wightman et al., 2013)

Flies expressing Gyk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 have a lifespan similar to that of wild-type controls when raised on a sucrose only food source. However, when raised on a glycerol only food source, the mutant flies have a reduced lifespan compared to wild-type flies raised under these conditions, with the lifespan being similar to that under starvation conditions. The mutant flies show an intermediate lifespan when raised on a food source containing glycerol and sucrose.

Adult males expressing Gyk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 have a small but significant decrease in survivorship on a high salt diet (3.5% and 4.0%) compared to control flies.

(Wightman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gk1^RNAi.UAS, Scer\GAL4^c564 has visible | pharate adult stage phenotype, enhanceable by Gpdh1^n1-4

(Wightman et al., 2013)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, enhanceable by bw¹/bw[+]

(Wightman et al., 2012)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, enhanceable by g[+]/g¹

(Wightman et al., 2012)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, enhanceable by ry¹/ry[+]

(Wightman et al., 2012)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, enhanceable by v[+]/v¹

(Wightman et al., 2012)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, enhanceable by y[+]/y¹

(Wightman et al., 2012)

NOT Enhanced by

Statement

Reference

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, non-enhanceable by bw¹⁶/bw[+]

(Wightman et al., 2012)

Suppressed by

Statement

Reference

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, suppressible by Gk2^UAS.Ex, Scer\GAL4^c564

(Wightman et al., 2012)

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, suppressible by bw[+]/bw¹⁹

(Wightman et al., 2012)

NOT suppressed by

Statement

Reference

Gk1^RNAi.UAS, Scer\GAL4^c564 has chemical sensitive phenotype, non-suppressible by bw¹⁶/bw[+]

(Wightman et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gk1^RNAi.UAS, Scer\GAL4^c564 has wing | pharate adult stage phenotype, enhanceable by Gpdh1^n1-4

(Wightman et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Gpdh^n1-4 enhances the curled or crumpled wing phenotype seen in Scer\GAL4^c564>Gk1^{dsRNA.Scer\UAS}-let escapers.

(Wightman et al., 2013)

Co-expression of Gk^Scer\UAS.Ex rescues the reduced survival on a glycerol only food source of flies expressing Gyk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564.

The glycerol hypersensitivity of flies expressing Gyk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564 (seen as a reduction in survival on a glycerol only food source) is enhanced if the flies are also heterozygous for any one of bw¹, g¹, ry¹, v¹ or y¹, is suppressed if the flies are heterozygous for bw¹⁹ and is not significantly affected if the flies are heterozygous for bw¹⁶.

(Wightman et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Co-expression of Gk1^Scer\UAS.Ex significantly reduces the pupal lethality seen with expression of Gk1^{dsRNA.Scer\UAS}-let driven by Scer\GAL4^c564.

(Wightman et al., 2013)

Co-expression of Gyk^Scer\UAS.Ex rescues the reduced survival on a glycerol only food source of flies expressing Gyk^{dsRNA.Scer\UAS} under the control of Scer\GAL4^c564.

(Wightman et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gk1^RNAi.UAS

Gk1^{dsRNA.Scer\UAS}

Gk1^dsRNA.UAS

Gyk^{dsRNA.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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