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General Information
Symbol
Dmel\Gk1dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0268039
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dGyk-IR
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of Gk1 sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Gk1dsRNA.Scer\UAS driven by Scer\GAL4αTub84B.PL results in larval lethality ('Gk2dsRNA.Scer\UAS-let') or survival to adulthood ('Gk1dsRNA.Scer\UAS-sur'), depending on the insertion line. Expression of Gk1dsRNA.Scer\UAS-let (but not Gk1dsRNA.Scer\UAS-sur) lines driven by Scer\GAL4c564 or Scer\GAL4how-24B also results in larval and pupal lethality. Scer\GAL4c564>Gk1dsRNA.Scer\UAS-let third instar larvae often show melanotic masses before lethality mainly at the pharate adult stage (escapers have a curled or crumpled wing phenotype). Expression of Gk1dsRNA.Scer\UAS driven by Scer\GAL4GMR.PS or Scer\GAL4elav-C155 does not result in any obvious external or behavioral phenotypes.

Flies expressing GykdsRNA.Scer\UAS under the control of Scer\GAL4c564 have a lifespan similar to that of wild-type controls when raised on a sucrose only food source. However, when raised on a glycerol only food source, the mutant flies have a reduced lifespan compared to wild-type flies raised under these conditions, with the lifespan being similar to that under starvation conditions. The mutant flies show an intermediate lifespan when raised on a food source containing glycerol and sucrose.

Adult males expressing GykdsRNA.Scer\UAS under the control of Scer\GAL4c564 have a small but significant decrease in survivorship on a high salt diet (3.5% and 4.0%) compared to control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Gpdhn1-4 enhances the curled or crumpled wing phenotype seen in Scer\GAL4c564>Gk1dsRNA.Scer\UAS-let escapers.

Co-expression of GkScer\UAS.Ex rescues the reduced survival on a glycerol only food source of flies expressing GykdsRNA.Scer\UAS under the control of Scer\GAL4c564.

The glycerol hypersensitivity of flies expressing GykdsRNA.Scer\UAS under the control of Scer\GAL4c564 (seen as a reduction in survival on a glycerol only food source) is enhanced if the flies are also heterozygous for any one of bw1, g1, ry1, v1 or y1, is suppressed if the flies are heterozygous for bw19 and is not significantly affected if the flies are heterozygous for bw16.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Co-expression of Gk1Scer\UAS.Ex significantly reduces the pupal lethality seen with expression of Gk1dsRNA.Scer\UAS-let driven by Scer\GAL4c564.

Co-expression of GykScer\UAS.Ex rescues the reduced survival on a glycerol only food source of flies expressing GykdsRNA.Scer\UAS under the control of Scer\GAL4c564.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Gk1dsRNA.Scer\UAS
Gk1dsRNA.UAS
GykdsRNA.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)