dysc^s168/dysc^s168 and dysc^s168/dysc^c03838 third instar larvae show a significant reduction in bouton number (both type 1b and type 1s boutons) and a significant increase in the size of type 1s boutons at the neuromuscular junction.

Homozygous larvae show a significant increase in the rate of body wall peristalsis compared to controls.

dysc^s168 homozygotes display arrhythmic locomotor patterns in constant dark conditions, with a minority showing weak rhythmicity. In contrast, the eclosion rhythm of these flies closely mirrors wild type controls.

dysc^s168/Df(3L)BSC614 animals display arrhythmic locomotor patterns in constant dark conditions.

In contrast to wild type flies, dysc^s168 homozygotes do not exhibit anticipation of lights-on in 12 h light:dark conditions, while anticipation of lights-off is maintained. Overall daytime and nighttime activity is greater in these mutants compared to wild type in these conditions.

dysc^s168/dysc^c03838 and dysc^s168/dysc^c05107 animals display arrhythmic locomotor patterns in constant dark conditions.

Compared to wild type, dysc^s168 mutant Ilp2-positive neurons in adult brains show a much greater reduction in the outward current when the membrane potential is held at -30 mV, indicating a loss of non-activating currents.

In contrast to wild type, the non-activating currents in dysc^s168 mutant Ilp2-positive neurons exhibit only a slight increase upon addition of CaCl[[2]].

Whereas application of 1 mM tetrathylammonium (a blocker of slo channels) reduces the total outward current by 63% in wild type neurons, it produces only a 17% reduction in dysc^s168 neurons.