FlyBase Allele Report: Scer\GAL4[ATP7.PB]

General Information

Symbol

Scer\GAL4^ATP7.PB

Species

S. cerevisiae

Name

FlyBase ID

FBal0281745

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{ATP7-GAL4.B}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Burke et al., 2008)

Progenitor genotype

Carried in construct

P{ATP7-GAL4.B}

Cytology

Description

GAL4 driver expression is driven by 608bp of ATP7 upstream sequence, from the translational start site of CG10347 (reverse orientation) to the translational start site of ATP7.

(Burke et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

ATP7

ATP7_5DAG4

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

larval brain

(Burke et al., 2008)

larval peripheral nervous system | segmentally repeated

(Burke et al., 2008)

mouthpart

(Burke et al., 2008)

third instar larval stage

interneuron | subset of larval abdominal neuromere

(Sellami et al., 2012)

peptidergic neuron | subset of larval abdominal neuromere

(Sellami et al., 2012)

peptidergic neuron | subset of embryonic/larval hypocerebral ganglion

(Sellami et al., 2012)

endocrine neuron | subset of retrocerebral complex

(Sellami et al., 2012)

larval insulin secreting cell of pars intercerebralis | subset

(Sellami et al., 2012)

endocrine neuron | subset of larval abdomen

(Sellami et al., 2012)

larval ventral nerve cord

(Burke et al., 2008)

embryonic/larval salivary gland

(Burke et al., 2008)

gonad

(Burke et al., 2008)

gastric caecum

(Burke et al., 2008)

embryonic/larval copper cell

(Burke et al., 2008)

embryonic/larval anterior midgut | restricted

(Burke et al., 2008)

embryonic/larval posterior midgut | restricted

(Burke et al., 2008)

embryonic/larval hindgut

(Burke et al., 2008)

embryonic/larval Malpighian tubule

(Burke et al., 2008)

larval Va Capability neuron

(Sellami et al., 2012)

adult stage

labellum

(Burke et al., 2008)

maxillary palp

(Burke et al., 2008)

third segment of antenna

(Burke et al., 2008)

leg sensillum

(Burke et al., 2008)

wing sensillum

(Burke et al., 2008)

adult gut

(Burke et al., 2008)

adult stage | male

testis

(Burke et al., 2008)

Additional Information

Statement

Reference

ScerGAL4^ATP7.PB colocalizes with Crz in interneurons in the larval abdominal neuromere, with sNPF in the hypocerebral ganglion, with Capa in the Va neurons, with Akh in endocrine neurons in the retrocerebral complex, with Ms in larval dorsomedial neurosecretory cells and endocrine neurons in the abdomen and with SIFa in larval dorsomedial neurosecretory cells.

(Sellami et al., 2012)

Reporter expression is observed in first instar larvae in the brain, in a segmentally repeated pattern in the PNS, in scattered enteric nervous system cells of the anterior midgut and in mouthparts. Expression is upregulated in the midgut at this stage in response to copper. In the third instar, expression is observed in the CNS (mainly in the ventral nerve cord but not in the optic lobe), in the salivary glands, and in the male gonads. Expression is also observed in the anterior and posterior midguts, hindgut and Malpighian tubules and is strongly upregulated upon exposure to copper. In adults, expression is observed in neurons of the labellum, maxillary palps, third antennal segment, leg, and wing margin as well as in the gut and testis.

(Burke et al., 2008)

Marker for

Reflects expression of

ATP7

(Sellami et al., 2012, Burke et al., 2008)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References