FlyBase Allele Report: Scer\GAL4[Ir76b.1.5]

General Information

Symbol

Scer\GAL4^Ir76b.1.5

Species

S. cerevisiae

Name

FlyBase ID

FBal0285659

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{Ir76b-GAL4.1.5}

Also Known As

Ir76b-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhang et al., 2013)

Progenitor genotype

Carried in construct

P{Ir76b-GAL4.1.5}

Cytology

Description

An approximately 1.5kb genomic fragment extending from upstream of the predicted Ir76b transcription start site is fused upstream of sequence encoding a GAL4 driver.

(Zhang et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

Ir76b

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

abdominal 2 ventral multidendritic neuron v'td1

(Qian et al., 2018)

abdominal 3 ventral multidendritic neuron v'td1

(Qian et al., 2018)

abdominal 4 ventral multidendritic neuron v'td1

(Qian et al., 2018)

abdominal 5 ventral multidendritic neuron v'td1

(Qian et al., 2018)

abdominal 6 ventral multidendritic neuron v'td1

(Qian et al., 2018)

adult stage

gustatory receptor neuron | subset of labellum

(Zhang et al., 2013, Zhang et al., 2013)

large labellar taste bristle

(Zhang et al., 2013)

adult gnathal ganglion | restricted

(Zhang et al., 2013, Zhang et al., 2013)

gustatory receptor neuron | subset of prothoracic tarsal segment

(Zhang et al., 2013)

gustatory receptor neuron | subset of wing margin

(Zhang et al., 2013)

adult antennal lobe | restricted

(Zhang et al., 2013)

adult ventral nerve cord | restricted

(Zhang et al., 2013)

pharyngeal sense organ

(Ganguly et al., 2017)

gustatory receptor neuron | subset of leg taste bristle

(Ganguly et al., 2017)

gustatory receptor neuron | subset of labellar taste peg

(Ganguly et al., 2017)

gustatory receptor neuron | subset of labellar taste bristle

(Ganguly et al., 2017)

low salt-sensing neuron of labellar taste bristle of labellar taste bristle

(Ganguly et al., 2017)

adult subesophageal zone | restricted

(Ganguly et al., 2017)

prothoracic leg taste bristle chemosensory neuron

(Ahn et al., 2017)

bitter-sensing neuron of the leg

(Ahn et al., 2017)

gustatory receptor neuron of the labellum

(Ahn et al., 2017)

short labellar taste bristle S-c

(Asefa et al., 2024)

bitter-sensing neuron of labellar taste bristle

(Pradhan et al., 2024)

gustatory receptor neuron

(Asefa et al., 2024)

Additional Information

Statement

Reference

Scer\GAL4^Ir76b.1.5 drives expression in gustatory neurons that do not detect sugar or express Gr64f in short labellar taste bristles S-c

(Asefa et al., 2024)

Scer\GAL4^Ir76b.1.5 drives expression in almost all labellar taste neurons.

(Asefa et al., 2024)

ScerGAL4^Ir76b.1.5 labels multiple cells of the labellum as well as axonal projections in the adult subesophageal zone. ScerGAL4^Ir76b.1.5 labelled cells have near complete overlap with expression driven by ScerGAL4^Ir76b.916.

(Ganguly et al., 2017)

Expression is observed in multiple gustatory receptor neurons per sensillum. In the tarsal sensilla, expression largely overlaps and includes sweet and bitter gustatory receptor neurons. Expression also occurs in multiple neurons per sensillum in the labial palps.

(Ahn et al., 2017)

Drives expression in almost all adult gustatory receptor neurons, and in a subset of multidendritic larval tracheal innervating neurons.

(FlyBase Curators, 2017)

Marker for

Reflects expression of

Ir76b

(Asefa et al., 2024, Ahn et al., 2017, Ganguly et al., 2017, Zhang et al., 2013, Zhang et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

gustatory receptor neuron | adult stage, with Ir20a^UAS.cGa

(Ganguly et al., 2017)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Ir76b.1.5 cells in adult females significantly reduces preference for oviposition on acid-containing medium, compared to controls; this phenotype is also seen when inhibition is restricted to a smaller subset of Scer\GAL4^Ir76b.1.5 cells (using Ecol\lexA^Gr66a.PU, Ecol\lexA^VP16.Gr64f and Scer\GAL80^{ts.lexAop.cTa}).

(Chen and Amrein, 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions