FlyBase Allele Report: Dmel\tsl[Δ]

General Information

Symbol

Dmel\tsl^Δ

Species

D. melanogaster

Name

FlyBase ID

FBal0290718

Feature type

allele

Associated gene

Dmel\tsl

Associated Insertion(s)

TI{TI}tsl^Δ

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Johnson et al., 2013)

Mutagen

ends-out gene targeting

(Johnson et al., 2013)

Progenitor genotype

Associated Insertion(s)

TI{TI}tsl^Δ

Cytology

Description

The entire tsl coding region has been replaced by a w^+mW.hs marker.

(Johnson et al., 2013)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:21,778,203..21,781,061 [-]

Comment:

Ends-out homologous recombination has resulted in the entire tsl coding region being replaced by a w marker. Deletion boundaries determined from primer sequences.

(Johnson et al., 2013)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Johnson et al., 2013)

abnormal developmental rate (with tsl⁵)

(Johnson et al., 2013)

abnormal size | adult stage

(Johnson et al., 2013)

abnormal size | third instar larval stage

(Johnson et al., 2013)

decreased cell size | adult stage | recessive

(Johnson et al., 2013)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect

(Johnson et al., 2017)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect (with tsl²)

(Johnson et al., 2017)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect (with tsl³)

(Johnson et al., 2017)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect (with tsl⁴)

(Johnson et al., 2017)

embryonic/larval segmentation phenotype | embryonic stage | maternal effect (with tsl⁵)

(Johnson et al., 2017)

female sterile

(Johnson et al., 2013)

viable

(Johnson et al., 2013)

visible | adult stage | recessive

(Johnson et al., 2013)

Phenotype Manifest In

abdominal segment 8 | embryonic stage | maternal effect

(Johnson et al., 2017)

abdominal segment 8 | embryonic stage | maternal effect (with tsl²)

(Johnson et al., 2017)

abdominal segment 8 | embryonic stage | maternal effect (with tsl³)

(Johnson et al., 2017)

abdominal segment 8 | embryonic stage | maternal effect (with tsl⁴)

(Johnson et al., 2017)

abdominal segment 8 | embryonic stage | maternal effect (with tsl⁵)

(Johnson et al., 2017)

amnioproctodeal invagination | maternal effect

(Johnson et al., 2017)

embryo

(Johnson et al., 2013)

embryonic/larval cuticle | embryonic stage | maternal effect (with tsl⁴)

(Johnson et al., 2017)

embryonic/larval cuticle | embryonic stage | maternal effect (with tsl⁵)

(Johnson et al., 2017)

embryonic/larval cuticle | embryonic stage | maternal effect | ventral

(Johnson et al., 2017)

embryonic head | maternal effect

(Johnson et al., 2017)

embryonic head | maternal effect (with tsl²)

(Johnson et al., 2017)

embryonic head | maternal effect (with tsl³)

(Johnson et al., 2017)

embryonic head | maternal effect (with tsl⁴)

(Johnson et al., 2017)

embryonic head | maternal effect (with tsl⁵)

(Johnson et al., 2017)

epithelial cell | embryonic stage | maternal effect | ventral

(Johnson et al., 2017)

filzkorper | embryonic stage | maternal effect

(Johnson et al., 2017)

filzkorper | embryonic stage | maternal effect (with tsl²)

(Johnson et al., 2017)

filzkorper | embryonic stage | maternal effect (with tsl³)

(Johnson et al., 2017)

filzkorper | embryonic stage | maternal effect (with tsl⁴)

(Johnson et al., 2017)

filzkorper | embryonic stage | maternal effect (with tsl⁵)

(Johnson et al., 2017)

mesoderm | embryonic stage | maternal effect | ventral

(Johnson et al., 2017)

primordial germ cell | embryonic stage | maternal effect

(Johnson et al., 2017)

telson | embryonic stage | maternal effect

(Johnson et al., 2017)

telson | embryonic stage | maternal effect (with tsl²)

(Johnson et al., 2017)

telson | embryonic stage | maternal effect (with tsl³)

(Johnson et al., 2017)

telson | embryonic stage | maternal effect (with tsl⁴)

(Johnson et al., 2017)

telson | embryonic stage | maternal effect (with tsl⁵)

(Johnson et al., 2017)

ventral furrow | maternal effect

(Johnson et al., 2017)

wing hair

(Johnson et al., 2013)

Detailed Description

Statement

Reference

Late embryos from tsl^Δ/tsl² tsl^Δ/tsl³, tsl^Δ/tsl⁴, tsl^Δ/tsl⁵ transheterozygous and tsl^Δ homozygous mothers exhibit a fully penetrant "terminal class mutant" phenotype, in which some terminal structures are missing (including head structures, abdominal segment 8, telson, and filzkorper), as compared to controls; late embryos from tsl^Δ/tsl⁴, tsl^Δ/tsl⁵ transheterozygous and tsl^Δ homozygous mothers also exhibit an increasingly highly penetrant posterior-ventral cuticle hole phenotype, as compared to controls.

Gastrulating embryos from tsl^Δ homozygous mothers present an elongated, irregular and often incomplete ventral furrow, associated with delayed and patchy apical constriction, misaligned apical edges and apical mis-positioning of the nucleus in ventral domain cells, as compared to controls; these defects are more pronounced at the posterior region of the embryo, which frequently shows a complete failure in apical constriction. These gastrulating embryos also present failed posterior midgut invagination, leaving the pole cells and a large field of Twist-positive mesodermal tissue exposed at the dorsal surface, as compared to controls.

(Johnson et al., 2017)

tsl^Δ mutant females are sterile, laying embryos that fail to specify terminal cell fate.

tsl^Δ mutant homozygotes show a significant delay of 26 hours to reach pupariation compared with heterozygous controls. The L2-L3 transition is delayed by ~7 hours. tsl^Δ mutant adult flies are 28% smaller than heterozygous controls and larvae are also noticeably smaller both at the wandering stage and throughout the third instar larval stage.

Wing cell density is significantly higher in tsl^Δ mutant flies compared with controls, indicating that cell size is reduced. Adult wings are also smaller than controls.

tsl^Δ/tsl⁵ mutant transheterozygotes are significantly delayed in reaching pupariation compared with controls.

(Johnson et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

tsl^Δ has abnormal developmental rate phenotype, enhanceable by tor^rv66

(Johnson et al., 2013)

NOT suppressed by

Statement

Reference

tsl^Δ has abnormal size phenotype, non-suppressible by tor^rv66

(Johnson et al., 2013)

Enhancer of

Statement

Reference

tsl^Δ is an enhancer of abnormal developmental rate phenotype of tor^rv66

(Johnson et al., 2013)

tsl^Δ is an enhancer of abnormal size phenotype of Ptth^UASp.Tag:HA, Scer\GAL4^phtm.PO

(Johnson et al., 2013)

Suppressor of

Statement

Reference

tsl^Δ is a suppressor of abnormal size phenotype of tor^rv66

(Johnson et al., 2013)

NOT Suppressor of

Statement

Reference

tsl^Δ is a non-suppressor of abnormal developmental rate phenotype of Ptth^UASp.Tag:HA, Scer\GAL4^phtm.PO

(Johnson et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

tsl^Δ has embryonic/larval cuticle | embryonic stage | ventral | maternal effect phenotype, enhanceable by RhoGEF2[+]/RhoGEF2^4.4

(Johnson et al., 2017)

NOT Enhanced by

Statement

Reference

tsl^Δ has embryonic/larval cuticle | embryonic stage | ventral | maternal effect phenotype, non-enhanceable by fog^UASp.cJa/Scer\GAL4^{VP16.nanos.UTR}

(Johnson et al., 2017)

Suppressor of

Statement

Reference

tsl^Δ/tsl^Δ is a suppressor | partially of embryonic/larval cuticle | maternal effect | embryonic stage phenotype of Scer\GAL4^{VP16.nanos.UTR}, fog^UASp.cJa

(Johnson et al., 2017)

NOT Suppressor of

Statement

Reference

tsl^Δ is a non-suppressor of embryo phenotype of Ptth^UASp.Tag:HA, Scer\GAL4^{VP16.nanos.UTR}

(Johnson et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The ventral cuticle defects displayed by late embryos from tsl^Δ homozygous mothers are strongly enhanced by heterozygosity for RhoGEF2^4.4, leading to fully penetrant absence of ventral cuticle.

Late embryos from mothers both homozygous for tsl^Δ and expressing fog^{Scer\UAS.P\T.cJa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} exhibit cuticle defects more similar to those of embryos from mothers only homozygous for tsl^Δ (i.e. some missing terminal structures and ventral-posterior cuticle hole) than those of embryos from mothers only expressing fog^{Scer\UAS.P\T.cJa} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} (i.e. only small amounts of recognizable cuticle remaining).

(Johnson et al., 2017)

tsl^Δ tor^rv66 double mutants show a dramatically extended larval period compared to either mutant alone, with flies pupating 83 hours after controls. Double mutant adult flies are similar in size to tsl^Δ mutants alone.

A tsl^Δ mutant background does not suppress the advanced pupariation phenotype seen in flies expressing Ptth^{Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^phm.PO. The decrease in male body size is further enhanced. The embryonic patterning defects seen when Ptth^{Scer\UAS.P\T.T:Ivir\HA1} is expressed under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} are also not suppressed.

(Johnson et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tsl^Δ is rescued by tsl^UAS.cJa/Scer\GAL4^da-BG00643

(Johnson et al., 2013)

Partially rescued by

tsl^Δ is partially rescued by tsl^Tag:HA

(Johnson et al., 2017)

tsl^Δ is partially rescued by tsl^{UAS.Tag:MYC,EGFP}/Scer\GAL4^slbo.2.6

(Johnson et al., 2017)

tsl^Δ is partially rescued by tsl^Tag:HA

(Johnson et al., 2013)

Not rescued by

tsl^Δ is not rescued by tsl^UAS.cJa/Scer\GAL4^phtm.PO

(Johnson et al., 2013)

Comments

The following defects exhibited by embryos from tsl^Δ mothers are partially rescued by one copy of tsl^T:Ivir\HA1 : the ventral cuticle hole, but not the "terminal class mutant" phenotype, in late embryos; both the irregularities/gaps in the ventral furrow and the exposure of mesodermal tissue (Twist-positive) at the dorsal surface, but not the elongated ventral furrow or the exposure of pole cells at the dorsal surface, in gastrulating embryos.

The expression of tsl^{Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP} under the control of Scer\GAL4^slbo.2.6 fully rescues the ventral cuticle hole and head defects, and partially rescues the patterning and cuticle defects exhibited by embryos from tsl^Δ mothers.

(Johnson et al., 2017)

Expression of tsl^Scer\UAS.cJa under the control of Scer\GAL4^da-BG00643 rescues the delay in pupariation seen in tsl^Δ mutants. Expression of tsl^Scer\UAS.cJa under the control of Scer\GAL4^phm.PO is unable to rescue the phenotype.

Expression of tsl^T:Ivir\HA1 completely rescues the developmental delay and reduction in size seen in tsl^Δ mutant flies. The defect in terminal embryonic patterning is not rescued.

(Johnson et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

tsl^Δ

(Taylor et al., 2019, Henstridge et al., 2018, Johnson et al., 2017, Johnson et al., 2015, Henstridge et al., 2014, Johnson et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (6)

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