FlyBase Allele Report: Dmel\ben[UAS.cUa]

General Information

Symbol

Dmel\ben^UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0294792

Feature type

allele

Associated gene

Dmel\ben

Associated Insertion(s)

Carried in Construct

P{UAS-ben.U}

Also Known As

UAS-Ben

Key Links

Transgenic product class

wild_type

(Uthaman et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Uthaman et al., 2008)

Mutagen

in vitro construct

(Uthaman et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-ben.U}

Cytology

Description

UAS regulatory sequences drive expression of ben coding sequences.

(Uthaman et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

ben

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

No obvious phenotypes are seen when ben^Scer\UAS.cUa is expressed under the control of Scer\GAL4^ptc-559.1.

(Ma et al., 2013)

Expression of ben^Scer\UAS.cUa both pre- and post-synaptically under the control of Scer\GAL4^OK307 does not affect synapse physiology.

(Uthaman et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/Traf6^RNAi.UAS

(Ma et al., 2013)

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/Tak1^NIG.1388R

(Ma et al., 2013)

Enhancer of

Statement

Reference

ben^UAS.cUa, Uev1A^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

NOT Enhancer of

Statement

Reference

ben^UAS.cUa/Scer\GAL4^GMR.PF is a non-enhancer of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has visible phenotype

(Ma et al., 2013)

Scer\GAL4^ptc-559.1, Traf6^RNAi.UAS, Uev1A^UAS.cMa, ben^UAS.cUa has visible phenotype

(Ma et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1/Traf6^RNAi.UAS

(Ma et al., 2013)

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1/Tak1^NIG.1388R

(Ma et al., 2013)

Enhancer of

Statement

Reference

ben^UAS.cUa, Uev1A^UAS.cMa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

NOT Enhancer of

Statement

Reference

ben^UAS.cUa/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Other

Statement

Reference

Scer\GAL4^ptc-559.1, Uev1A^UAS.cMa, ben^UAS.cUa has anterior crossvein phenotype

(Ma et al., 2013)

Scer\GAL4^ptc-559.1, Traf6^RNAi.UAS, Uev1A^UAS.cMa, ben^UAS.cUa has eye phenotype

(Ma et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa under the control of Scer\GAL4^ptc-559.1 results in loss of the anterior cross vein.

Expression of Traf6^{dsRNA.Scer\UAS} suppresses the anterior crossvein loss seen when Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa are expressed under the control of Scer\GAL4^ptc-559.1.

Expression of Tak1^NIG.1388R suppresses the anterior crossvein loss seen when Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa are expressed under the control of Scer\GAL4^ptc-559.1.

Co-expression of Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa in the eye under the control of Scer\GAL4^GMR.PF does not results in any significant phenotype.

Co-expression of Uev1A^Scer\UAS.cMa, ben^Scer\UAS.cUa and Traf6^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in rough eye with fused ommatidia.

(Ma et al., 2013)

Co-expression of ben^Scer\UAS.cUa does not enhance the small, rough eye phenotype found upon expression of nopo^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

Co-expression of Uev1A^Scer\UAS.cMa and ben^Scer\UAS.cUa significantly enhances the Scer\GAL4^GMR.PF->nopo^Scer\UAS.cUa small rough eye phenotype.

(Ma et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ben^UAS.cUa/Scer\GAL4^shot-OK307 rescues ben¹

(Uthaman et al., 2008)

Scer\GAL4^c17/ben^UAS.cUa rescues ben¹

(Uthaman et al., 2008)

Fails to rescue

ben^UAS.cUa/Scer\GAL4^c42.2 fails to rescue ben¹

(Uthaman et al., 2008)

Scer\GAL4^{shakB.lethal.4.1}/ben^UAS.cUa fails to rescue ben¹

(Uthaman et al., 2008)

Comments

Expression of ben^Scer\UAS.cUa under the control of Scer\GAL4^OK307 rescues the ben¹ physiological and anatomical phenotypes.

Expression of ben^Scer\UAS.cUa under the control of Scer\GAL4^c17 rescues the ben¹ physiological and anatomical phenotypes on 83% of flies. This increases to 91% when two ben^Scer\UAS.cUa transgenes are used.

Pre-synaptic expression of ben^Scer\UAS.cUa under the control of Scer\GAL4^c42.2, which turns on expression in the later half of pupal development when the giant fiber would be undergoing additional growth after having made its synapse in wild-type specimens, fails to rescue the ben¹ physiological and anatomical phenotypes.

Expression of ben^Scer\UAS.cUa under the control of the postsynaptic Scer\GAL4^{shakB.lethal.4.1} driver, fails to rescue the ben¹ mutant phenotype.

(Uthaman et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ben^Scer\UAS.cUa

ben^UAS.cUa

Name Synonyms

Saccharomyces cerevisiae UAS construct of Uthaman

(Uthaman et al., 2008)

Secondary FlyBase IDs

References (4)

Report Sections

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