FlyBase Allele Report: Scer\GAL4[VT044966]

General Information

Symbol

Scer\GAL4^VT044966

Species

S. cerevisiae

Name

FlyBase ID

FBal0307887

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{VT044966-GAL4}

Also Known As

VT44966-Gal4, VT44966

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Meadows, 2015.3.13)

Progenitor genotype

Carried in construct

P{VT044966-GAL4}

Cytology

Description

A 2334 base pair fragment from the flanking non-coding or intronic region of CG31191 is fused upstream of a Drosophila synthetic core promoter (DSCP) followed by sequence encoding a GAL4 driver.

(Meadows, 2015.3.13)

Allele components

Component

Use(s)

Regulatory region(s)

VT044966

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | :conditional | heat sensitive, with TrpA1^UAS.(B).cKa

(Zhao et al., 2018)

abnormal courtship behavior | conditional, with Ctet\tetX^TNT-LC.UAS

(Zhao et al., 2018)

abnormal memory | adult stage | heat sensitive, with shi^1.UAS

(Yang et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adult males with inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^VT044966 cells do not have significant suppression of courtship behavior following training with mated females, unlike controls.

Adult males with thermogenetic activation (using TrpA1^UAS.(B).cKa at 32[o]C) of Scer\GAL4^VT044966 cells have a significant suppression of courtship behavior following training with a virgin female, unlike controls; there is no suppression of courtship behavior due to activation in the absence of training, similar to controls.

(Zhao et al., 2018)

Inhibition (using shi^1.UAS at 31[o]C) of Scer\GAL4^VT044966 cells in adults during testing only significantly reduces 3-hour aversive olfactory memory, but not anaesthesia-resistant memory, compared to controls, with no effect on odor or shock avoidance.

(Yang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Dop1R1^f02676/Scer\GAL4^VT044966 fails to rescue Dop1R1^f02676

(Shyu et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v203571

P{VT044966-GAL4}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^VT044966

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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